Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay

A sandwich ELISA for the detection of Echinococcus granulosus coproantigen in formalin and heat-treated faecal supernatants of dogs was developed. The assay used affinity-purified polyclonal antibodies obtained from rabbits hyperimmunised with E. granulosus excretory/secretory antigens and biotiayla...

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Veröffentlicht in:International journal for parasitology 1997-12, Vol.27 (12), p.1605-1612
Hauptverfasser: Malgor, R., Nonaka, N., Basmadjian, I., Sakai, H., Carámbula, B., Oku, Y., Carmona, C., Kamiya, M.
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container_end_page 1612
container_issue 12
container_start_page 1605
container_title International journal for parasitology
container_volume 27
creator Malgor, R.
Nonaka, N.
Basmadjian, I.
Sakai, H.
Carámbula, B.
Oku, Y.
Carmona, C.
Kamiya, M.
description A sandwich ELISA for the detection of Echinococcus granulosus coproantigen in formalin and heat-treated faecal supernatants of dogs was developed. The assay used affinity-purified polyclonal antibodies obtained from rabbits hyperimmunised with E. granulosus excretory/secretory antigens and biotiaylated monoclonal antibody EmA9 produced against adult E. multilocularis somatic extract. The test was sensitive to 7 ng and 2.3 ng of E. granulosus protein and carbohydrate/ml of faecal supernatant, respectively. Thirteen helminth-free dogs were infected with different amounts of E. granulosus protoscoleces and the presence of coproantigen was monitored during the prepatent period until day 35 post-infection, when they were necropsied. Faecal antigen levels started to rise above the normal range between days 10 and 20 post-infection, and typically peaked at the end of the experiment. All the dogs, bearing from 3 to 67 700 worms, showed positive values in the ELISA during the prepatent period. One dog experimentally infected with Taenia hydatigena metacestode and harbouring three worms, tested positive only after the prepatent period at day 52. The test was applied to 98 stray dogs. The ELISA detected all of four dogs naturally infected with E. granulosus, two dogs with patent infections of T. hydatigena and two dogs with no cestode infections, sbowing a sensitivity of 100% and a specificity of 96%.
doi_str_mv 10.1016/S0020-7519(97)00127-6
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One dog experimentally infected with Taenia hydatigena metacestode and harbouring three worms, tested positive only after the prepatent period at day 52. The test was applied to 98 stray dogs. 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The assay used affinity-purified polyclonal antibodies obtained from rabbits hyperimmunised with E. granulosus excretory/secretory antigens and biotiaylated monoclonal antibody EmA9 produced against adult E. multilocularis somatic extract. The test was sensitive to 7 ng and 2.3 ng of E. granulosus protein and carbohydrate/ml of faecal supernatant, respectively. Thirteen helminth-free dogs were infected with different amounts of E. granulosus protoscoleces and the presence of coproantigen was monitored during the prepatent period until day 35 post-infection, when they were necropsied. Faecal antigen levels started to rise above the normal range between days 10 and 20 post-infection, and typically peaked at the end of the experiment. All the dogs, bearing from 3 to 67 700 worms, showed positive values in the ELISA during the prepatent period. One dog experimentally infected with Taenia hydatigena metacestode and harbouring three worms, tested positive only after the prepatent period at day 52. The test was applied to 98 stray dogs. The ELISA detected all of four dogs naturally infected with E. granulosus, two dogs with patent infections of T. hydatigena and two dogs with no cestode infections, sbowing a sensitivity of 100% and a specificity of 96%.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9467748</pmid><doi>10.1016/S0020-7519(97)00127-6</doi><tpages>8</tpages></addata></record>
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subjects Animal helminthic diseases
Animals
Antibodies, Helminth - immunology
Antibodies, Monoclonal - immunology
Antigens, Helminth - analysis
Biological and medical sciences
coproantigen
diagnosis
Dog Diseases - diagnosis
Dogs
Echinococcosis - diagnosis
Echinococcosis - veterinary
Echinococcus - immunology
Echinococcus - isolation & purification
Echinococcus granulosus
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Feces - parasitology
Female
Helminthic diseases
Immunohistochemistry
Infectious diseases
Male
Medical sciences
Parasitic diseases
Sensitivity and Specificity
title Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay
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