Capillary isoelectric focusing

Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on‐tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability. This...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Electrophoresis 1997, Vol.18 (12-13), p.2134-2144
Hauptverfasser:	Rodriguez-Diaz, Roberto, Wehr, Tim, Zhu, Mingde
Format:	Artikel
Sprache:	eng
Schlagworte:	Capillary isoelectric focusing Electrophoresis, Capillary - methods Forecasting Humans Isoelectric Focusing - methods Mass Spectrometry Proteins - analysis Reference Standards
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	2144
container_issue	12-13
container_start_page	2134
container_title	Electrophoresis
container_volume	18
creator	Rodriguez-Diaz, Roberto Wehr, Tim Zhu, Mingde
description	Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on‐tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability. This review summarizes improvements in sample preparation, capillary selection, and focusing and zone mobilization procedures which have helped CIEF become a more robust analytical method. New applications are reviewed, in particular the use of CIEF in analysis of biopharmaceutical products.
doi_str_mv	10.1002/elps.1150181204
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79550590</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79550590</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4484-a3750964d232e68f59e771e0fad0b7b43e052efa68e1f1d042064a9e501980b93</originalsourceid><addsrcrecordid>eNqFkE1Lw0AURQdRaq2uXSlduUv75iuZwZXWtgqhKioFN8MkeZFo2sRMg_bfm5JSceXqLd69h8sh5JTCgAKwIealG1AqgSrKQOyRLpWMecxXfJ90gQbcA8XlITly7h0AhBaiQzpaSB-Y6pLzkS2zPLfVup-5AnOMV1UW99Mirl22fDsmB6nNHZ5sb4-8TMbPo1svvJ_eja5CLxZCCc_yQIL2RcI4Q1-lUmMQUITUJhAFkeAIkmFqfYU0pQkIBr6wGpvZWkGkeY9ctNyyKj5rdCuzyFyMzbAlFrUzgZYSpIYmOGyDcVU4V2FqyipbNPMNBbMxYjZGzK-RpnG2RdfRApNdfqug-V-2_68sx_V_ODMOH57-0L22nbkVfu_atvowftBYMfPZ1NzMrh_hdRKaOf8Bhkh7Ag</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79550590</pqid></control><display><type>article</type><title>Capillary isoelectric focusing</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Rodriguez-Diaz, Roberto ; Wehr, Tim ; Zhu, Mingde</creator><creatorcontrib>Rodriguez-Diaz, Roberto ; Wehr, Tim ; Zhu, Mingde</creatorcontrib><description>Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on‐tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability. This review summarizes improvements in sample preparation, capillary selection, and focusing and zone mobilization procedures which have helped CIEF become a more robust analytical method. New applications are reviewed, in particular the use of CIEF in analysis of biopharmaceutical products.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.1150181204</identifier><identifier>PMID: 9456028</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Capillary isoelectric focusing ; Electrophoresis, Capillary - methods ; Forecasting ; Humans ; Isoelectric Focusing - methods ; Mass Spectrometry ; Proteins - analysis ; Reference Standards</subject><ispartof>Electrophoresis, 1997, Vol.18 (12-13), p.2134-2144</ispartof><rights>Copyright © 1997 VCH Verlagsgesellschaft mbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4484-a3750964d232e68f59e771e0fad0b7b43e052efa68e1f1d042064a9e501980b93</citedby><cites>FETCH-LOGICAL-c4484-a3750964d232e68f59e771e0fad0b7b43e052efa68e1f1d042064a9e501980b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felps.1150181204$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.1150181204$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9456028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodriguez-Diaz, Roberto</creatorcontrib><creatorcontrib>Wehr, Tim</creatorcontrib><creatorcontrib>Zhu, Mingde</creatorcontrib><title>Capillary isoelectric focusing</title><title>Electrophoresis</title><addtitle>ELECTROPHORESIS</addtitle><description>Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on‐tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability. This review summarizes improvements in sample preparation, capillary selection, and focusing and zone mobilization procedures which have helped CIEF become a more robust analytical method. New applications are reviewed, in particular the use of CIEF in analysis of biopharmaceutical products.</description><subject>Capillary isoelectric focusing</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Forecasting</subject><subject>Humans</subject><subject>Isoelectric Focusing - methods</subject><subject>Mass Spectrometry</subject><subject>Proteins - analysis</subject><subject>Reference Standards</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1Lw0AURQdRaq2uXSlduUv75iuZwZXWtgqhKioFN8MkeZFo2sRMg_bfm5JSceXqLd69h8sh5JTCgAKwIealG1AqgSrKQOyRLpWMecxXfJ90gQbcA8XlITly7h0AhBaiQzpaSB-Y6pLzkS2zPLfVup-5AnOMV1UW99Mirl22fDsmB6nNHZ5sb4-8TMbPo1svvJ_eja5CLxZCCc_yQIL2RcI4Q1-lUmMQUITUJhAFkeAIkmFqfYU0pQkIBr6wGpvZWkGkeY9ctNyyKj5rdCuzyFyMzbAlFrUzgZYSpIYmOGyDcVU4V2FqyipbNPMNBbMxYjZGzK-RpnG2RdfRApNdfqug-V-2_68sx_V_ODMOH57-0L22nbkVfu_atvowftBYMfPZ1NzMrh_hdRKaOf8Bhkh7Ag</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Rodriguez-Diaz, Roberto</creator><creator>Wehr, Tim</creator><creator>Zhu, Mingde</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>Capillary isoelectric focusing</title><author>Rodriguez-Diaz, Roberto ; Wehr, Tim ; Zhu, Mingde</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4484-a3750964d232e68f59e771e0fad0b7b43e052efa68e1f1d042064a9e501980b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Capillary isoelectric focusing</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Forecasting</topic><topic>Humans</topic><topic>Isoelectric Focusing - methods</topic><topic>Mass Spectrometry</topic><topic>Proteins - analysis</topic><topic>Reference Standards</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodriguez-Diaz, Roberto</creatorcontrib><creatorcontrib>Wehr, Tim</creatorcontrib><creatorcontrib>Zhu, Mingde</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodriguez-Diaz, Roberto</au><au>Wehr, Tim</au><au>Zhu, Mingde</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary isoelectric focusing</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>1997</date><risdate>1997</risdate><volume>18</volume><issue>12-13</issue><spage>2134</spage><epage>2144</epage><pages>2134-2144</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Capillary isoelectric focusing (CIEF) provides excellent resolution of proteins with the advantage that separations are carried out in a capillary format with on‐tube detection and automated analysis. Recent advances in the technique provide improved resolution, reproducibility and reliability. This review summarizes improvements in sample preparation, capillary selection, and focusing and zone mobilization procedures which have helped CIEF become a more robust analytical method. New applications are reviewed, in particular the use of CIEF in analysis of biopharmaceutical products.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>9456028</pmid><doi>10.1002/elps.1150181204</doi><tpages>11</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0173-0835
ispartof	Electrophoresis, 1997, Vol.18 (12-13), p.2134-2144
issn	0173-0835 1522-2683
language	eng
recordid	cdi_proquest_miscellaneous_79550590
source	MEDLINE; Wiley Journals
subjects	Capillary isoelectric focusing Electrophoresis, Capillary - methods Forecasting Humans Isoelectric Focusing - methods Mass Spectrometry Proteins - analysis Reference Standards
title	Capillary isoelectric focusing
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T14%3A40%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Capillary%20isoelectric%20focusing&rft.jtitle=Electrophoresis&rft.au=Rodriguez-Diaz,%20Roberto&rft.date=1997&rft.volume=18&rft.issue=12-13&rft.spage=2134&rft.epage=2144&rft.pages=2134-2144&rft.issn=0173-0835&rft.eissn=1522-2683&rft_id=info:doi/10.1002/elps.1150181204&rft_dat=%3Cproquest_cross%3E79550590%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79550590&rft_id=info:pmid/9456028&rfr_iscdi=true