Increased gelatinase A (MMP-2) and gelatinase B (MMP-9) activities in human brain after focal ischemia
Matrix metalloproteinases (MMPs) are involved in remodelling extracellular matrix. Gelatinase B (MMP-9) is an inducible 92 kDa MMP expressed by neutrophils, microglia, and endothelial cells. Gelatinase A (MMP-2) is a 72 kDa MMP, constitutively expressed in brain. Elevated MMP activity has been linke...
Gespeichert in:
| Veröffentlicht in: | Neuroscience letters 1997-11, Vol.238 (1), p.53-56 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 56 |
|---|---|
| container_issue | 1 |
| container_start_page | 53 |
| container_title | Neuroscience letters |
| container_volume | 238 |
| creator | Clark, Arthur W Krekoski, Craig A Bou, Shao-Sun Chapman, Kevin R Edwards, Dylan R |
| description | Matrix metalloproteinases (MMPs) are involved in remodelling extracellular matrix. Gelatinase B (MMP-9) is an inducible 92 kDa MMP expressed by neutrophils, microglia, and endothelial cells. Gelatinase A (MMP-2) is a 72 kDa MMP, constitutively expressed in brain. Elevated MMP activity has been linked to various pathologic conditions, and the therapeutic benefit of MMP inhibitors is under study in a few experimental models. Using gelatin zymography, we have compared activities of these MMPs in infarcted and matched non-infarcted cerebral tissue from eight subjects dying at intervals of less than 2 h to several years after a stroke. Gelatinase B activity was markedly elevated in the infarcted tissue at two days post-infarction, and remained elevated in cases dying months after the event. Increases in gelatinase A activity were subtle at 2–5 days; they were marked and significant in cases dying at 4 months and later. The findings indicate distinct temporal profiles of post-ischemic gelatinase activity in human brain, with earlier but equally persistent elevation in gelatinase B when compared to gelatinase A. |
| doi_str_mv | 10.1016/S0304-3940(97)00859-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79550431</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304394097008598</els_id><sourcerecordid>16405763</sourcerecordid><originalsourceid>FETCH-LOGICAL-c538t-ec5bdcb06c87eea0f0db89054f5192f94e14f7f2c5076fd2a9b73f53842073d23</originalsourceid><addsrcrecordid>eNqFkc1LHTEUxUNp0af2TxCyKEUXY2--JpNVsaJVUBTUdchkbjRlPmwyT-h_b57v8aArV7lwfie5OYeQQwYnDFj94x4EyEoYCUdGHwM0ylTNJ7JgjeaVNpp_Jostskv2cv4DAIopuUN2jKxlrcSChKvRJ3QZO_qEvZvjWGZ6So9ubu4qfkzd-J_way2YIvg5vsY5YqZxpM_LwY20Ta7MLsyYaJi862nM_hmH6A7Il-D6jF835z55vDh_OLusrm9_X52dXldeiWau0Ku28y3UvtGIDgJ0bWNAyaCY4cFIZDLowL0CXYeOO9NqEYpVctCi42KffF_f-5Kmv0vMsx3KCtj3bsRpma02SoEU7EOQ1RKUrkUB1Rr0aco5YbAvKQ4u_bMM7KoI-16EXaVsjbbvRdim-A43DyzbAbuta5N80b9tdJdLUiG50ce8xTiUBvXqQz_XGJbUXiMmm33E0WMXE_rZdlP8YJE3JImiUg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16405763</pqid></control><display><type>article</type><title>Increased gelatinase A (MMP-2) and gelatinase B (MMP-9) activities in human brain after focal ischemia</title><source>MEDLINE</source><source>Elsevier ScienceDirect</source><creator>Clark, Arthur W ; Krekoski, Craig A ; Bou, Shao-Sun ; Chapman, Kevin R ; Edwards, Dylan R</creator><creatorcontrib>Clark, Arthur W ; Krekoski, Craig A ; Bou, Shao-Sun ; Chapman, Kevin R ; Edwards, Dylan R</creatorcontrib><description>Matrix metalloproteinases (MMPs) are involved in remodelling extracellular matrix. Gelatinase B (MMP-9) is an inducible 92 kDa MMP expressed by neutrophils, microglia, and endothelial cells. Gelatinase A (MMP-2) is a 72 kDa MMP, constitutively expressed in brain. Elevated MMP activity has been linked to various pathologic conditions, and the therapeutic benefit of MMP inhibitors is under study in a few experimental models. Using gelatin zymography, we have compared activities of these MMPs in infarcted and matched non-infarcted cerebral tissue from eight subjects dying at intervals of less than 2 h to several years after a stroke. Gelatinase B activity was markedly elevated in the infarcted tissue at two days post-infarction, and remained elevated in cases dying months after the event. Increases in gelatinase A activity were subtle at 2–5 days; they were marked and significant in cases dying at 4 months and later. The findings indicate distinct temporal profiles of post-ischemic gelatinase activity in human brain, with earlier but equally persistent elevation in gelatinase B when compared to gelatinase A.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(97)00859-8</identifier><identifier>PMID: 9464653</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Aged ; Aged, 80 and over ; Biological and medical sciences ; Brain Ischemia - enzymology ; Cerebral ischemia ; Collagenases - metabolism ; Female ; Gelatinase A ; Gelatinase B ; Gelatinases - metabolism ; Humans ; Male ; Matrix metalloproteinase ; Matrix Metalloproteinase 2 ; Matrix Metalloproteinase 9 ; Medical sciences ; Metalloendopeptidases - metabolism ; Middle Aged ; MMP-2 ; MMP-9 ; Neurology ; Stroke ; Time Factors ; Vascular diseases and vascular malformations of the nervous system</subject><ispartof>Neuroscience letters, 1997-11, Vol.238 (1), p.53-56</ispartof><rights>1997 Elsevier Science Ireland Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-ec5bdcb06c87eea0f0db89054f5192f94e14f7f2c5076fd2a9b73f53842073d23</citedby><cites>FETCH-LOGICAL-c538t-ec5bdcb06c87eea0f0db89054f5192f94e14f7f2c5076fd2a9b73f53842073d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3940(97)00859-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2097272$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9464653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clark, Arthur W</creatorcontrib><creatorcontrib>Krekoski, Craig A</creatorcontrib><creatorcontrib>Bou, Shao-Sun</creatorcontrib><creatorcontrib>Chapman, Kevin R</creatorcontrib><creatorcontrib>Edwards, Dylan R</creatorcontrib><title>Increased gelatinase A (MMP-2) and gelatinase B (MMP-9) activities in human brain after focal ischemia</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Matrix metalloproteinases (MMPs) are involved in remodelling extracellular matrix. Gelatinase B (MMP-9) is an inducible 92 kDa MMP expressed by neutrophils, microglia, and endothelial cells. Gelatinase A (MMP-2) is a 72 kDa MMP, constitutively expressed in brain. Elevated MMP activity has been linked to various pathologic conditions, and the therapeutic benefit of MMP inhibitors is under study in a few experimental models. Using gelatin zymography, we have compared activities of these MMPs in infarcted and matched non-infarcted cerebral tissue from eight subjects dying at intervals of less than 2 h to several years after a stroke. Gelatinase B activity was markedly elevated in the infarcted tissue at two days post-infarction, and remained elevated in cases dying months after the event. Increases in gelatinase A activity were subtle at 2–5 days; they were marked and significant in cases dying at 4 months and later. The findings indicate distinct temporal profiles of post-ischemic gelatinase activity in human brain, with earlier but equally persistent elevation in gelatinase B when compared to gelatinase A.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Brain Ischemia - enzymology</subject><subject>Cerebral ischemia</subject><subject>Collagenases - metabolism</subject><subject>Female</subject><subject>Gelatinase A</subject><subject>Gelatinase B</subject><subject>Gelatinases - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 2</subject><subject>Matrix Metalloproteinase 9</subject><subject>Medical sciences</subject><subject>Metalloendopeptidases - metabolism</subject><subject>Middle Aged</subject><subject>MMP-2</subject><subject>MMP-9</subject><subject>Neurology</subject><subject>Stroke</subject><subject>Time Factors</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1LHTEUxUNp0af2TxCyKEUXY2--JpNVsaJVUBTUdchkbjRlPmwyT-h_b57v8aArV7lwfie5OYeQQwYnDFj94x4EyEoYCUdGHwM0ylTNJ7JgjeaVNpp_Jostskv2cv4DAIopuUN2jKxlrcSChKvRJ3QZO_qEvZvjWGZ6So9ubu4qfkzd-J_way2YIvg5vsY5YqZxpM_LwY20Ta7MLsyYaJi862nM_hmH6A7Il-D6jF835z55vDh_OLusrm9_X52dXldeiWau0Ku28y3UvtGIDgJ0bWNAyaCY4cFIZDLowL0CXYeOO9NqEYpVctCi42KffF_f-5Kmv0vMsx3KCtj3bsRpma02SoEU7EOQ1RKUrkUB1Rr0aco5YbAvKQ4u_bMM7KoI-16EXaVsjbbvRdim-A43DyzbAbuta5N80b9tdJdLUiG50ce8xTiUBvXqQz_XGJbUXiMmm33E0WMXE_rZdlP8YJE3JImiUg</recordid><startdate>19971128</startdate><enddate>19971128</enddate><creator>Clark, Arthur W</creator><creator>Krekoski, Craig A</creator><creator>Bou, Shao-Sun</creator><creator>Chapman, Kevin R</creator><creator>Edwards, Dylan R</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19971128</creationdate><title>Increased gelatinase A (MMP-2) and gelatinase B (MMP-9) activities in human brain after focal ischemia</title><author>Clark, Arthur W ; Krekoski, Craig A ; Bou, Shao-Sun ; Chapman, Kevin R ; Edwards, Dylan R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-ec5bdcb06c87eea0f0db89054f5192f94e14f7f2c5076fd2a9b73f53842073d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Brain Ischemia - enzymology</topic><topic>Cerebral ischemia</topic><topic>Collagenases - metabolism</topic><topic>Female</topic><topic>Gelatinase A</topic><topic>Gelatinase B</topic><topic>Gelatinases - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 2</topic><topic>Matrix Metalloproteinase 9</topic><topic>Medical sciences</topic><topic>Metalloendopeptidases - metabolism</topic><topic>Middle Aged</topic><topic>MMP-2</topic><topic>MMP-9</topic><topic>Neurology</topic><topic>Stroke</topic><topic>Time Factors</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clark, Arthur W</creatorcontrib><creatorcontrib>Krekoski, Craig A</creatorcontrib><creatorcontrib>Bou, Shao-Sun</creatorcontrib><creatorcontrib>Chapman, Kevin R</creatorcontrib><creatorcontrib>Edwards, Dylan R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clark, Arthur W</au><au>Krekoski, Craig A</au><au>Bou, Shao-Sun</au><au>Chapman, Kevin R</au><au>Edwards, Dylan R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased gelatinase A (MMP-2) and gelatinase B (MMP-9) activities in human brain after focal ischemia</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1997-11-28</date><risdate>1997</risdate><volume>238</volume><issue>1</issue><spage>53</spage><epage>56</epage><pages>53-56</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Matrix metalloproteinases (MMPs) are involved in remodelling extracellular matrix. Gelatinase B (MMP-9) is an inducible 92 kDa MMP expressed by neutrophils, microglia, and endothelial cells. Gelatinase A (MMP-2) is a 72 kDa MMP, constitutively expressed in brain. Elevated MMP activity has been linked to various pathologic conditions, and the therapeutic benefit of MMP inhibitors is under study in a few experimental models. Using gelatin zymography, we have compared activities of these MMPs in infarcted and matched non-infarcted cerebral tissue from eight subjects dying at intervals of less than 2 h to several years after a stroke. Gelatinase B activity was markedly elevated in the infarcted tissue at two days post-infarction, and remained elevated in cases dying months after the event. Increases in gelatinase A activity were subtle at 2–5 days; they were marked and significant in cases dying at 4 months and later. The findings indicate distinct temporal profiles of post-ischemic gelatinase activity in human brain, with earlier but equally persistent elevation in gelatinase B when compared to gelatinase A.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>9464653</pmid><doi>10.1016/S0304-3940(97)00859-8</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1997-11, Vol.238 (1), p.53-56 |
| issn | 0304-3940 1872-7972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79550431 |
| source | MEDLINE; Elsevier ScienceDirect |
| subjects | Aged Aged, 80 and over Biological and medical sciences Brain Ischemia - enzymology Cerebral ischemia Collagenases - metabolism Female Gelatinase A Gelatinase B Gelatinases - metabolism Humans Male Matrix metalloproteinase Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Medical sciences Metalloendopeptidases - metabolism Middle Aged MMP-2 MMP-9 Neurology Stroke Time Factors Vascular diseases and vascular malformations of the nervous system |
| title | Increased gelatinase A (MMP-2) and gelatinase B (MMP-9) activities in human brain after focal ischemia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-03T07%3A11%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20gelatinase%20A%20(MMP-2)%20and%20gelatinase%20B%20(MMP-9)%20activities%20in%20human%20brain%20after%20focal%20ischemia&rft.jtitle=Neuroscience%20letters&rft.au=Clark,%20Arthur%20W&rft.date=1997-11-28&rft.volume=238&rft.issue=1&rft.spage=53&rft.epage=56&rft.pages=53-56&rft.issn=0304-3940&rft.eissn=1872-7972&rft.coden=NELED5&rft_id=info:doi/10.1016/S0304-3940(97)00859-8&rft_dat=%3Cproquest_cross%3E16405763%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16405763&rft_id=info:pmid/9464653&rft_els_id=S0304394097008598&rfr_iscdi=true |