Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 beta

Bovine rIL-1 beta (rbIL-1 beta) was instilled intrabronchially into the lungs of steers to elicit harvestable alveolar neutrophils for functional analysis. Before instillation, bronchoalveolar lavage samples from the steers consisted of 96.4 +/- 1.5% (mean +/- SEM) macrophages, with the remaining ce...

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Veröffentlicht in:The Journal of immunology (1950) 1990-02, Vol.144 (3), p.1037-1041
Hauptverfasser: Heidel, JR, Sassenfeld, HM, Maliszewdki, CR, Silflow, RM, Baker, PE, Taylor, SM, Leid, RW
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container_end_page 1041
container_issue 3
container_start_page 1037
container_title The Journal of immunology (1950)
container_volume 144
creator Heidel, JR
Sassenfeld, HM
Maliszewdki, CR
Silflow, RM
Baker, PE
Taylor, SM
Leid, RW
description Bovine rIL-1 beta (rbIL-1 beta) was instilled intrabronchially into the lungs of steers to elicit harvestable alveolar neutrophils for functional analysis. Before instillation, bronchoalveolar lavage samples from the steers consisted of 96.4 +/- 1.5% (mean +/- SEM) macrophages, with the remaining cells neutrophils and occasional lymphocytes. Four hours after instillation of 1.0 and 10.0 nmol of IL-1, the lavage samples consisted of 96.3 +/- 0.8% and 91.0 +/- 5.7% neutrophils, respectively. Alveolar neutrophils elicited with rbIL-1 beta and challenged with the calcium ionophore, A23187, released similar amounts of leukotriene B4 (LTB4) and its nonenzymatic isomer LTB I, and significantly greater amounts of 5-hydroxyeicosatetraenoic acid and the nonenzymatic isomer LTB II, when compared with circulating neutrophils. The rbIL-1 beta did not, by itself, stimulate release of arachidonate metabolites from circulating neutrophils in quantities that were detectable by HPLC. Circulating neutrophils, preincubated with rbIL-1 beta and stimulated with A23187, released significantly greater amounts of 5-hydroxyeicosatetraenoic acid and total 5-lipoxygenase metabolites when compared with control cells not incubated with rbIL-1 beta. Incubation of circulating neutrophils with rbIL-1 beta and A23187 concurrently resulted in a significantly increased release of all 5-lipoxygenase metabolites of arachidonate. However, both the release of superoxide anion and bacterial killing by rbIL-1 beta-elicited bovine alveolar neutrophils did not differ from the values obtained for circulating neutrophils.
doi_str_mv 10.4049/jimmunol.144.3.1037
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Circulating neutrophils, preincubated with rbIL-1 beta and stimulated with A23187, released significantly greater amounts of 5-hydroxyeicosatetraenoic acid and total 5-lipoxygenase metabolites when compared with control cells not incubated with rbIL-1 beta. Incubation of circulating neutrophils with rbIL-1 beta and A23187 concurrently resulted in a significantly increased release of all 5-lipoxygenase metabolites of arachidonate. However, both the release of superoxide anion and bacterial killing by rbIL-1 beta-elicited bovine alveolar neutrophils did not differ from the values obtained for circulating neutrophils.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.144.3.1037</identifier><identifier>PMID: 2153175</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Analysis of the immune response. 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Psychology ; Fundamental immunology ; Hydroxyeicosatetraenoic Acids - metabolism ; Immunobiology ; Interleukin-1 - pharmacology ; Leukotrienes - metabolism ; Neutrophils - physiology ; Organs and cells involved in the immune response ; Pulmonary Alveoli - cytology ; Recombinant Proteins ; Superoxides - metabolism</subject><ispartof>The Journal of immunology (1950), 1990-02, Vol.144 (3), p.1037-1041</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-aca2d8bde6e3a21abad6d957b3923179cbcf1c5bd6ba923091aa71102c30e2523</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=5347656$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2153175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heidel, JR</creatorcontrib><creatorcontrib>Sassenfeld, HM</creatorcontrib><creatorcontrib>Maliszewdki, CR</creatorcontrib><creatorcontrib>Silflow, RM</creatorcontrib><creatorcontrib>Baker, PE</creatorcontrib><creatorcontrib>Taylor, SM</creatorcontrib><creatorcontrib>Leid, RW</creatorcontrib><title>Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 beta</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Bovine rIL-1 beta (rbIL-1 beta) was instilled intrabronchially into the lungs of steers to elicit harvestable alveolar neutrophils for functional analysis. Before instillation, bronchoalveolar lavage samples from the steers consisted of 96.4 +/- 1.5% (mean +/- SEM) macrophages, with the remaining cells neutrophils and occasional lymphocytes. Four hours after instillation of 1.0 and 10.0 nmol of IL-1, the lavage samples consisted of 96.3 +/- 0.8% and 91.0 +/- 5.7% neutrophils, respectively. Alveolar neutrophils elicited with rbIL-1 beta and challenged with the calcium ionophore, A23187, released similar amounts of leukotriene B4 (LTB4) and its nonenzymatic isomer LTB I, and significantly greater amounts of 5-hydroxyeicosatetraenoic acid and the nonenzymatic isomer LTB II, when compared with circulating neutrophils. The rbIL-1 beta did not, by itself, stimulate release of arachidonate metabolites from circulating neutrophils in quantities that were detectable by HPLC. Circulating neutrophils, preincubated with rbIL-1 beta and stimulated with A23187, released significantly greater amounts of 5-hydroxyeicosatetraenoic acid and total 5-lipoxygenase metabolites when compared with control cells not incubated with rbIL-1 beta. Incubation of circulating neutrophils with rbIL-1 beta and A23187 concurrently resulted in a significantly increased release of all 5-lipoxygenase metabolites of arachidonate. However, both the release of superoxide anion and bacterial killing by rbIL-1 beta-elicited bovine alveolar neutrophils did not differ from the values obtained for circulating neutrophils.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Arachidonate 5-Lipoxygenase - metabolism</subject><subject>Arachidonic Acid</subject><subject>Arachidonic Acids - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blood Bactericidal Activity</subject><subject>Bronchoalveolar Lavage Fluid</subject><subject>Cattle</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hydroxyeicosatetraenoic Acids - metabolism</subject><subject>Immunobiology</subject><subject>Interleukin-1 - pharmacology</subject><subject>Leukotrienes - metabolism</subject><subject>Neutrophils - physiology</subject><subject>Organs and cells involved in the immune response</subject><subject>Pulmonary Alveoli - cytology</subject><subject>Recombinant Proteins</subject><subject>Superoxides - metabolism</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9rFDEchoModa1-AhFyED3Nmv-zc5TSamHBi96E8Esm46ZkkjXJdPDbm7Lb4s1TIO_zvgkPQm8p2Qoihk93fp6XmMKWCrHlW0p4_wxtqJSkU4qo52hDCGMd7VX_Er0q5Y4QoggTF-iCUclpLzfo580SbfUpQsClLqN3BacJm3Tvo8MQ7l0KkHF0S83pePChYBe89dWNePX1gLOzaTY-QqyPrdt9R7FxFV6jFxOE4t6cz0v04-b6-9XXbv_ty-3V531n-cBqBxbYuDOjU44Do2BgVOMge9PS9svBGjtRK82oDLQbMlCAnlLCLCeOScYv0YfT7jGn34srVc--WBcCRJeWovtBCkF3w39BKsWODIo3kJ9Am1Mp2U36mP0M-Y-mRD_I14_ydZOvuX6Q31rvzvOLmd341Dnbbvn7cw7FQpgyROvLEya56JVUDft4wg7-12H12ekyQwhtlOp1Xf958C_Lqp2y</recordid><startdate>19900201</startdate><enddate>19900201</enddate><creator>Heidel, JR</creator><creator>Sassenfeld, HM</creator><creator>Maliszewdki, CR</creator><creator>Silflow, RM</creator><creator>Baker, PE</creator><creator>Taylor, SM</creator><creator>Leid, RW</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19900201</creationdate><title>Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 beta</title><author>Heidel, JR ; Sassenfeld, HM ; Maliszewdki, CR ; Silflow, RM ; Baker, PE ; Taylor, SM ; Leid, RW</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-aca2d8bde6e3a21abad6d957b3923179cbcf1c5bd6ba923091aa71102c30e2523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Arachidonate 5-Lipoxygenase - metabolism</topic><topic>Arachidonic Acid</topic><topic>Arachidonic Acids - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blood Bactericidal Activity</topic><topic>Bronchoalveolar Lavage Fluid</topic><topic>Cattle</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hydroxyeicosatetraenoic Acids - metabolism</topic><topic>Immunobiology</topic><topic>Interleukin-1 - pharmacology</topic><topic>Leukotrienes - metabolism</topic><topic>Neutrophils - physiology</topic><topic>Organs and cells involved in the immune response</topic><topic>Pulmonary Alveoli - cytology</topic><topic>Recombinant Proteins</topic><topic>Superoxides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heidel, JR</creatorcontrib><creatorcontrib>Sassenfeld, HM</creatorcontrib><creatorcontrib>Maliszewdki, CR</creatorcontrib><creatorcontrib>Silflow, RM</creatorcontrib><creatorcontrib>Baker, PE</creatorcontrib><creatorcontrib>Taylor, SM</creatorcontrib><creatorcontrib>Leid, RW</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heidel, JR</au><au>Sassenfeld, HM</au><au>Maliszewdki, CR</au><au>Silflow, RM</au><au>Baker, PE</au><au>Taylor, SM</au><au>Leid, RW</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 beta</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1990-02-01</date><risdate>1990</risdate><volume>144</volume><issue>3</issue><spage>1037</spage><epage>1041</epage><pages>1037-1041</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>Bovine rIL-1 beta (rbIL-1 beta) was instilled intrabronchially into the lungs of steers to elicit harvestable alveolar neutrophils for functional analysis. Before instillation, bronchoalveolar lavage samples from the steers consisted of 96.4 +/- 1.5% (mean +/- SEM) macrophages, with the remaining cells neutrophils and occasional lymphocytes. Four hours after instillation of 1.0 and 10.0 nmol of IL-1, the lavage samples consisted of 96.3 +/- 0.8% and 91.0 +/- 5.7% neutrophils, respectively. Alveolar neutrophils elicited with rbIL-1 beta and challenged with the calcium ionophore, A23187, released similar amounts of leukotriene B4 (LTB4) and its nonenzymatic isomer LTB I, and significantly greater amounts of 5-hydroxyeicosatetraenoic acid and the nonenzymatic isomer LTB II, when compared with circulating neutrophils. The rbIL-1 beta did not, by itself, stimulate release of arachidonate metabolites from circulating neutrophils in quantities that were detectable by HPLC. Circulating neutrophils, preincubated with rbIL-1 beta and stimulated with A23187, released significantly greater amounts of 5-hydroxyeicosatetraenoic acid and total 5-lipoxygenase metabolites when compared with control cells not incubated with rbIL-1 beta. Incubation of circulating neutrophils with rbIL-1 beta and A23187 concurrently resulted in a significantly increased release of all 5-lipoxygenase metabolites of arachidonate. However, both the release of superoxide anion and bacterial killing by rbIL-1 beta-elicited bovine alveolar neutrophils did not differ from the values obtained for circulating neutrophils.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>2153175</pmid><doi>10.4049/jimmunol.144.3.1037</doi><tpages>5</tpages></addata></record>
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subjects Analysis of the immune response. Humoral and cellular immunity
Animals
Arachidonate 5-Lipoxygenase - metabolism
Arachidonic Acid
Arachidonic Acids - metabolism
Biological and medical sciences
Blood Bactericidal Activity
Bronchoalveolar Lavage Fluid
Cattle
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hydroxyeicosatetraenoic Acids - metabolism
Immunobiology
Interleukin-1 - pharmacology
Leukotrienes - metabolism
Neutrophils - physiology
Organs and cells involved in the immune response
Pulmonary Alveoli - cytology
Recombinant Proteins
Superoxides - metabolism
title Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 beta
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