Construction of a Panel of Canine–Rodent Hybrid Cell Lines for Use in Partitioning of the Canine Genome
We have constructed a collection of canine–rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescencein situhybridization and PCR analysis of canine microsa...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1997-12, Vol.46 (3), p.317-325 |
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| container_title | Genomics (San Diego, Calif.) |
| container_volume | 46 |
| creator | Langston, Amelia A. Mellersh, Cathryn S. Neal, Cassandra L. Ray, Kunal Acland, Gregory M. Gibbs, Mark Aguirre, Gustavo D. Fournier, R.E.K. Ostrander, Elaine A. |
| description | We have constructed a collection of canine–rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescencein situhybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome. |
| doi_str_mv | 10.1006/geno.1997.5009 |
| format | Article |
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Characterization of the hybrid cell lines using a combination of fluorescencein situhybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1997.5009</identifier><identifier>PMID: 9441734</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; CELL CULTURE ; Cell Line ; CHIEN ; CHROMOSOME ; CHROMOSOME ANALYSIS ; Chromosome Mapping ; CHROMOSOMES ; Classical genetics, quantitative genetics, hybrids ; Cricetinae ; Cricetulus ; CRICETULUS BARABENSIS ; CROMOSOMAS ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; DNA HYBRIDIZATION ; DOGS ; Dogs - genetics ; FIBROBLASTE ; FIBROBLASTOS ; FIBROBLASTS ; Fibroblasts - cytology ; FLUORESCENCE IN SITU HYBRIDIZATION ; Fundamental and applied biological sciences. Psychology ; GENE ; GENE LOCATION ; GENES ; GENETIC MARKERS ; Genetics of eukaryotes. Biological and molecular evolution ; Genome ; HIBRIDACION DE ADN ; HIBRIDOS ; Hybrid Cells ; HYBRIDATION D'ADN ; HYBRIDE ; HYBRIDS ; In Situ Hybridization, Fluorescence ; INDUCED CELL FUSION ; LOCALISATION DE GENE ; LOCALIZACION DE GENES ; Male ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; MICE ; MICROSATELITES ; MICROSATELLITE ; Microsatellite Repeats ; MICROSATELLITES ; PERRO ; Polymerase Chain Reaction ; RATON ; RODENTIA ; SOURIS ; Vertebrata</subject><ispartof>Genomics (San Diego, Calif.), 1997-12, Vol.46 (3), p.317-325</ispartof><rights>1997 Academic Press</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-f63a943a1832f3e643eaf004312d7b507d3d58a05e8bf603ce131d3395f703803</citedby><cites>FETCH-LOGICAL-c527t-f63a943a1832f3e643eaf004312d7b507d3d58a05e8bf603ce131d3395f703803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.1997.5009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2120817$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9441734$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Langston, Amelia A.</creatorcontrib><creatorcontrib>Mellersh, Cathryn S.</creatorcontrib><creatorcontrib>Neal, Cassandra L.</creatorcontrib><creatorcontrib>Ray, Kunal</creatorcontrib><creatorcontrib>Acland, Gregory M.</creatorcontrib><creatorcontrib>Gibbs, Mark</creatorcontrib><creatorcontrib>Aguirre, Gustavo D.</creatorcontrib><creatorcontrib>Fournier, R.E.K.</creatorcontrib><creatorcontrib>Ostrander, Elaine A.</creatorcontrib><title>Construction of a Panel of Canine–Rodent Hybrid Cell Lines for Use in Partitioning of the Canine Genome</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>We have constructed a collection of canine–rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescencein situhybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CELL CULTURE</subject><subject>Cell Line</subject><subject>CHIEN</subject><subject>CHROMOSOME</subject><subject>CHROMOSOME ANALYSIS</subject><subject>Chromosome Mapping</subject><subject>CHROMOSOMES</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>CRICETULUS BARABENSIS</subject><subject>CROMOSOMAS</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>DNA HYBRIDIZATION</subject><subject>DOGS</subject><subject>Dogs - genetics</subject><subject>FIBROBLASTE</subject><subject>FIBROBLASTOS</subject><subject>FIBROBLASTS</subject><subject>Fibroblasts - cytology</subject><subject>FLUORESCENCE IN SITU HYBRIDIZATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENE LOCATION</subject><subject>GENES</subject><subject>GENETIC MARKERS</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genome</subject><subject>HIBRIDACION DE ADN</subject><subject>HIBRIDOS</subject><subject>Hybrid Cells</subject><subject>HYBRIDATION D'ADN</subject><subject>HYBRIDE</subject><subject>HYBRIDS</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>INDUCED CELL FUSION</subject><subject>LOCALISATION DE GENE</subject><subject>LOCALIZACION DE GENES</subject><subject>Male</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>MICE</subject><subject>MICROSATELITES</subject><subject>MICROSATELLITE</subject><subject>Microsatellite Repeats</subject><subject>MICROSATELLITES</subject><subject>PERRO</subject><subject>Polymerase Chain Reaction</subject><subject>RATON</subject><subject>RODENTIA</subject><subject>SOURIS</subject><subject>Vertebrata</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGOFCEURYlxMrajW3cmLIy7aqGAglqayjhj0olmZnpNaHi0mGoYodpkdv6Df-iXSKUrvTOuILnnXS7vIvSGkjUlpPuwh5jWtO_lWhDSP0MrSlTfqI53z9GKKKUaKTh7gV6W8p1Ugqn2El32nFPJ-AqFIcUy5aOdQoo4eWzwVxNhnK-DiSHCn1-_75KDOOHbp10ODg8wjnhTlYJ9ynhbAIdYp_IUZpMQ9_Pw9A0WA3xTIx7gFbrwZizwejmv0PbT9cNw22y-3HwePm4aK1o5Nb5jpufMUMVaz6DjDIwnhDPaOrkTRDrmhDJEgNr5jjALlFHHWC-8JEwRdoXen3wfc_pxhDLpQyi2Zq7fSseiZS9YJfl_QdoxIbkUFVyfQJtTKRm8fszhYPKTpkTPJei5BD2XoOcS6sDbxfm4O4A748vWq_5u0U2xZvTZRBvKGWtpS1QFzzbeJG32uSLb-_kVIgQXqurqpENd588AWRcbIFpwIYOdtEvhXwn_AmxRqus</recordid><startdate>19971215</startdate><enddate>19971215</enddate><creator>Langston, Amelia A.</creator><creator>Mellersh, Cathryn S.</creator><creator>Neal, Cassandra L.</creator><creator>Ray, Kunal</creator><creator>Acland, Gregory M.</creator><creator>Gibbs, Mark</creator><creator>Aguirre, Gustavo D.</creator><creator>Fournier, R.E.K.</creator><creator>Ostrander, Elaine A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19971215</creationdate><title>Construction of a Panel of Canine–Rodent Hybrid Cell Lines for Use in Partitioning of the Canine Genome</title><author>Langston, Amelia A. ; Mellersh, Cathryn S. ; Neal, Cassandra L. ; Ray, Kunal ; Acland, Gregory M. ; Gibbs, Mark ; Aguirre, Gustavo D. ; Fournier, R.E.K. ; Ostrander, Elaine A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c527t-f63a943a1832f3e643eaf004312d7b507d3d58a05e8bf603ce131d3395f703803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CELL CULTURE</topic><topic>Cell Line</topic><topic>CHIEN</topic><topic>CHROMOSOME</topic><topic>CHROMOSOME ANALYSIS</topic><topic>Chromosome Mapping</topic><topic>CHROMOSOMES</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>CRICETULUS BARABENSIS</topic><topic>CROMOSOMAS</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>DNA HYBRIDIZATION</topic><topic>DOGS</topic><topic>Dogs - genetics</topic><topic>FIBROBLASTE</topic><topic>FIBROBLASTOS</topic><topic>FIBROBLASTS</topic><topic>Fibroblasts - cytology</topic><topic>FLUORESCENCE IN SITU HYBRIDIZATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENE LOCATION</topic><topic>GENES</topic><topic>GENETIC MARKERS</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genome</topic><topic>HIBRIDACION DE ADN</topic><topic>HIBRIDOS</topic><topic>Hybrid Cells</topic><topic>HYBRIDATION D'ADN</topic><topic>HYBRIDE</topic><topic>HYBRIDS</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>INDUCED CELL FUSION</topic><topic>LOCALISATION DE GENE</topic><topic>LOCALIZACION DE GENES</topic><topic>Male</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>MICE</topic><topic>MICROSATELITES</topic><topic>MICROSATELLITE</topic><topic>Microsatellite Repeats</topic><topic>MICROSATELLITES</topic><topic>PERRO</topic><topic>Polymerase Chain Reaction</topic><topic>RATON</topic><topic>RODENTIA</topic><topic>SOURIS</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Langston, Amelia A.</creatorcontrib><creatorcontrib>Mellersh, Cathryn S.</creatorcontrib><creatorcontrib>Neal, Cassandra L.</creatorcontrib><creatorcontrib>Ray, Kunal</creatorcontrib><creatorcontrib>Acland, Gregory M.</creatorcontrib><creatorcontrib>Gibbs, Mark</creatorcontrib><creatorcontrib>Aguirre, Gustavo D.</creatorcontrib><creatorcontrib>Fournier, R.E.K.</creatorcontrib><creatorcontrib>Ostrander, Elaine A.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Langston, Amelia A.</au><au>Mellersh, Cathryn S.</au><au>Neal, Cassandra L.</au><au>Ray, Kunal</au><au>Acland, Gregory M.</au><au>Gibbs, Mark</au><au>Aguirre, Gustavo D.</au><au>Fournier, R.E.K.</au><au>Ostrander, Elaine A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a Panel of Canine–Rodent Hybrid Cell Lines for Use in Partitioning of the Canine Genome</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1997-12-15</date><risdate>1997</risdate><volume>46</volume><issue>3</issue><spage>317</spage><epage>325</epage><pages>317-325</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>We have constructed a collection of canine–rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescencein situhybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>9441734</pmid><doi>10.1006/geno.1997.5009</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics (San Diego, Calif.), 1997-12, Vol.46 (3), p.317-325 |
| issn | 0888-7543 1089-8646 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79537034 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences CELL CULTURE Cell Line CHIEN CHROMOSOME CHROMOSOME ANALYSIS Chromosome Mapping CHROMOSOMES Classical genetics, quantitative genetics, hybrids Cricetinae Cricetulus CRICETULUS BARABENSIS CROMOSOMAS CULTIVO DE CELULAS CULTURE DE CELLULE DNA HYBRIDIZATION DOGS Dogs - genetics FIBROBLASTE FIBROBLASTOS FIBROBLASTS Fibroblasts - cytology FLUORESCENCE IN SITU HYBRIDIZATION Fundamental and applied biological sciences. Psychology GENE GENE LOCATION GENES GENETIC MARKERS Genetics of eukaryotes. Biological and molecular evolution Genome HIBRIDACION DE ADN HIBRIDOS Hybrid Cells HYBRIDATION D'ADN HYBRIDE HYBRIDS In Situ Hybridization, Fluorescence INDUCED CELL FUSION LOCALISATION DE GENE LOCALIZACION DE GENES Male MARCADORES GENETICOS MARQUEUR GENETIQUE MICE MICROSATELITES MICROSATELLITE Microsatellite Repeats MICROSATELLITES PERRO Polymerase Chain Reaction RATON RODENTIA SOURIS Vertebrata |
| title | Construction of a Panel of Canine–Rodent Hybrid Cell Lines for Use in Partitioning of the Canine Genome |
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