Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts
Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts were studied. In bulls, no immunoreaction was seen after treatment with antibodies to CA-I, -II and CA-III in the testis. Specific staining for CA-III, however, was evident in...
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| Veröffentlicht in: | Okajimas folia anatomica japonica 1997-12, Vol.74 (5), p.193-198 |
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| creator | Ichihara, N Sasaki, K Iseri, S Kimura, H Miura, K Nishita, T Asari, M |
| description | Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts were studied. In bulls, no immunoreaction was seen after treatment with antibodies to CA-I, -II and CA-III in the testis. Specific staining for CA-III, however, was evident in peritubular cells in interstitial tissue of the testis, epididymis. CA-II activity could be detected in epithelium of the epididymis, ductus deferentis and ampulla ductus deferentis. Especially, a strong reaction for CA-II was seen in apical in epithelium of the epididymis in the initial and middle segment. CA-I activity was only founded in ductus deferentis and ampulla ductus deferentis. No or a weak reaction for CA-I, CA-II and CA-III were seen in the three accessory reproductive glands. Specific immunostaining for CA-II and CA-I could be observed in the organ, suggesting the bicarbonate in bovine semen to derive primarily from the genital tract and not accessory reproductive organs. CA-III-positive peritubular cells in interstitial tissue were also stained for alpha smooth muscle actin, and were very similar to contractile myofibroblast cells (Wrobel et al., 1979). |
| doi_str_mv | 10.2535/ofaj1936.74.5_193 |
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In bulls, no immunoreaction was seen after treatment with antibodies to CA-I, -II and CA-III in the testis. Specific staining for CA-III, however, was evident in peritubular cells in interstitial tissue of the testis, epididymis. CA-II activity could be detected in epithelium of the epididymis, ductus deferentis and ampulla ductus deferentis. Especially, a strong reaction for CA-II was seen in apical in epithelium of the epididymis in the initial and middle segment. CA-I activity was only founded in ductus deferentis and ampulla ductus deferentis. No or a weak reaction for CA-I, CA-II and CA-III were seen in the three accessory reproductive glands. Specific immunostaining for CA-II and CA-I could be observed in the organ, suggesting the bicarbonate in bovine semen to derive primarily from the genital tract and not accessory reproductive organs. CA-III-positive peritubular cells in interstitial tissue were also stained for alpha smooth muscle actin, and were very similar to contractile myofibroblast cells (Wrobel et al., 1979).</description><identifier>ISSN: 0030-154X</identifier><identifier>DOI: 10.2535/ofaj1936.74.5_193</identifier><identifier>PMID: 9446931</identifier><language>eng</language><publisher>Japan</publisher><subject>Actinin - metabolism ; Animals ; Antibody Specificity ; Carbonic Anhydrases - metabolism ; Cattle ; Cytosol - enzymology ; Genitalia, Male - enzymology ; Immunohistochemistry ; Isoenzymes - metabolism ; Leydig Cells - enzymology ; Male</subject><ispartof>Okajimas folia anatomica japonica, 1997-12, Vol.74 (5), p.193-198</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9446931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ichihara, N</creatorcontrib><creatorcontrib>Sasaki, K</creatorcontrib><creatorcontrib>Iseri, S</creatorcontrib><creatorcontrib>Kimura, H</creatorcontrib><creatorcontrib>Miura, K</creatorcontrib><creatorcontrib>Nishita, T</creatorcontrib><creatorcontrib>Asari, M</creatorcontrib><title>Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts</title><title>Okajimas folia anatomica japonica</title><addtitle>Okajimas Folia Anat Jpn</addtitle><description>Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts were studied. In bulls, no immunoreaction was seen after treatment with antibodies to CA-I, -II and CA-III in the testis. Specific staining for CA-III, however, was evident in peritubular cells in interstitial tissue of the testis, epididymis. CA-II activity could be detected in epithelium of the epididymis, ductus deferentis and ampulla ductus deferentis. Especially, a strong reaction for CA-II was seen in apical in epithelium of the epididymis in the initial and middle segment. CA-I activity was only founded in ductus deferentis and ampulla ductus deferentis. No or a weak reaction for CA-I, CA-II and CA-III were seen in the three accessory reproductive glands. Specific immunostaining for CA-II and CA-I could be observed in the organ, suggesting the bicarbonate in bovine semen to derive primarily from the genital tract and not accessory reproductive organs. CA-III-positive peritubular cells in interstitial tissue were also stained for alpha smooth muscle actin, and were very similar to contractile myofibroblast cells (Wrobel et al., 1979).</description><subject>Actinin - metabolism</subject><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Carbonic Anhydrases - metabolism</subject><subject>Cattle</subject><subject>Cytosol - enzymology</subject><subject>Genitalia, Male - enzymology</subject><subject>Immunohistochemistry</subject><subject>Isoenzymes - metabolism</subject><subject>Leydig Cells - enzymology</subject><subject>Male</subject><issn>0030-154X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkE9LAzEQxXNQaq1-AA9CTqLg1qTJZjfHUvyzUPCi4G1JshOastnUZLfQfnpX2su8B-_HMPMQuqNkvshZ_hKs2lLJxLzg87we3QWaEsJIRnP-c4WuU9oSwktJyQRNJOdCMjpFsfJ-6MLGpT60wajWHVXvQoeDxUZFHTpnsOo2hyaqBNilcDx4SPhxtcyqZ_w_qzFvTq56wq7DOuxdB9irFnCEXQzNYHq3B9xHZfp0gy6tahPcnnWGvt9ev1Yf2frzvVot19mOLmifCdBWC2o5aYpSFxaogjwv-YI2tjSCjZ8IASCJolLTopBalIYYYU2jWM4sm6GH097xgt8BUl97lwy0reogDKku5FgbJXIE78_goD009S46r-KhPpfE_gDPVmiq</recordid><startdate>199712</startdate><enddate>199712</enddate><creator>Ichihara, N</creator><creator>Sasaki, K</creator><creator>Iseri, S</creator><creator>Kimura, H</creator><creator>Miura, K</creator><creator>Nishita, T</creator><creator>Asari, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199712</creationdate><title>Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts</title><author>Ichihara, N ; Sasaki, K ; Iseri, S ; Kimura, H ; Miura, K ; Nishita, T ; Asari, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p121t-6ebfb61f40d78b7fe1ae558421df8c6389166ee90a19b1779b68c0c6fcda353f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Actinin - metabolism</topic><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Carbonic Anhydrases - metabolism</topic><topic>Cattle</topic><topic>Cytosol - enzymology</topic><topic>Genitalia, Male - enzymology</topic><topic>Immunohistochemistry</topic><topic>Isoenzymes - metabolism</topic><topic>Leydig Cells - enzymology</topic><topic>Male</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichihara, N</creatorcontrib><creatorcontrib>Sasaki, K</creatorcontrib><creatorcontrib>Iseri, S</creatorcontrib><creatorcontrib>Kimura, H</creatorcontrib><creatorcontrib>Miura, K</creatorcontrib><creatorcontrib>Nishita, T</creatorcontrib><creatorcontrib>Asari, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Okajimas folia anatomica japonica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichihara, N</au><au>Sasaki, K</au><au>Iseri, S</au><au>Kimura, H</au><au>Miura, K</au><au>Nishita, T</au><au>Asari, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts</atitle><jtitle>Okajimas folia anatomica japonica</jtitle><addtitle>Okajimas Folia Anat Jpn</addtitle><date>1997-12</date><risdate>1997</risdate><volume>74</volume><issue>5</issue><spage>193</spage><epage>198</epage><pages>193-198</pages><issn>0030-154X</issn><abstract>Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts were studied. In bulls, no immunoreaction was seen after treatment with antibodies to CA-I, -II and CA-III in the testis. Specific staining for CA-III, however, was evident in peritubular cells in interstitial tissue of the testis, epididymis. CA-II activity could be detected in epithelium of the epididymis, ductus deferentis and ampulla ductus deferentis. Especially, a strong reaction for CA-II was seen in apical in epithelium of the epididymis in the initial and middle segment. CA-I activity was only founded in ductus deferentis and ampulla ductus deferentis. No or a weak reaction for CA-I, CA-II and CA-III were seen in the three accessory reproductive glands. Specific immunostaining for CA-II and CA-I could be observed in the organ, suggesting the bicarbonate in bovine semen to derive primarily from the genital tract and not accessory reproductive organs. CA-III-positive peritubular cells in interstitial tissue were also stained for alpha smooth muscle actin, and were very similar to contractile myofibroblast cells (Wrobel et al., 1979).</abstract><cop>Japan</cop><pmid>9446931</pmid><doi>10.2535/ofaj1936.74.5_193</doi><tpages>6</tpages></addata></record> |
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| subjects | Actinin - metabolism Animals Antibody Specificity Carbonic Anhydrases - metabolism Cattle Cytosol - enzymology Genitalia, Male - enzymology Immunohistochemistry Isoenzymes - metabolism Leydig Cells - enzymology Male |
| title | Immunohistolocalization of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in bovine male reproductive tracts |
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