Expression of sex hormone-binding globulin exon VII splicing variant mRNA in human uterine endometrium
We have demonstrated the expression of sex hormone-binding globulin (SHBG) exon VII splicing variant mRNA in human uterine endometrium, using the reverse transcription-polymerase chain reaction-Southern blot and DNA sequencing analyses. Analysis of the missing base pairs corresponded to the entire e...
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| Veröffentlicht in: | The Journal of steroid biochemistry and molecular biology 1997-08, Vol.62 (5), p.385-390 |
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| container_end_page | 390 |
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| container_issue | 5 |
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| container_title | The Journal of steroid biochemistry and molecular biology |
| container_volume | 62 |
| creator | Misao, Ryou Fujimoto, Jiro Nakanishi, Yoshihito Tamaya, Teruhiko |
| description | We have demonstrated the expression of sex hormone-binding globulin (SHBG) exon VII splicing variant mRNA in human uterine endometrium, using the reverse transcription-polymerase chain reaction-Southern blot and DNA sequencing analyses. Analysis of the missing base pairs corresponded to the entire exon VII, which are considered to encode a portion of the steroid-binding site. Therefore, the steroid-binding affinity of this variant might be different from that of the SHBG wild type. In uterine endometria, the wild-type and variant mRNA levels tended to increase with the advance of the menstrual phase, but the ratio of the SHBG variant mRNA to SHBG wild-type mRNA levels showed no significant difference during the menstrual cycle. So far, there are no indications that the SHBG variant has any biological or clinical implications in human uterine endometrium. |
| doi_str_mv | 10.1016/S0960-0760(97)00051-4 |
| format | Article |
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Analysis of the missing base pairs corresponded to the entire exon VII, which are considered to encode a portion of the steroid-binding site. Therefore, the steroid-binding affinity of this variant might be different from that of the SHBG wild type. In uterine endometria, the wild-type and variant mRNA levels tended to increase with the advance of the menstrual phase, but the ratio of the SHBG variant mRNA to SHBG wild-type mRNA levels showed no significant difference during the menstrual cycle. 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Analysis of the missing base pairs corresponded to the entire exon VII, which are considered to encode a portion of the steroid-binding site. Therefore, the steroid-binding affinity of this variant might be different from that of the SHBG wild type. In uterine endometria, the wild-type and variant mRNA levels tended to increase with the advance of the menstrual phase, but the ratio of the SHBG variant mRNA to SHBG wild-type mRNA levels showed no significant difference during the menstrual cycle. So far, there are no indications that the SHBG variant has any biological or clinical implications in human uterine endometrium.</description><subject>Adult</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Biological and medical sciences</subject><subject>DNA Primers - genetics</subject><subject>Endometrium - metabolism</subject><subject>Exons</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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Physiology</subject><subject>Polymerase Chain Reaction</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sex Hormone-Binding Globulin - genetics</subject><subject>Sex Hormone-Binding Globulin - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLJDEUhYM4aPv4CUIWg-iixqSSSlKrQURnGkTB1zakUrc0Q1XSk1RJ--9N201vXV0u57sPzkHohJJflFBx8UhqQQoiBTmr5TkhpKIF30EzqmRd0LIku2i2RfbRQUr_MsQYlXtor-a8Ljmdoe56uYiQkgsehw4nWOK3EIfgoWicb51_xa99aKbeeQzLDL3M5zgtemdX0ruJzvgRDw93lzgTb9NgPJ5GiM4DBt-GAcbopuEI_ehMn-B4Uw_R883109Xf4vb-z_zq8rawvCRjYSxtKtKq3LTGNJKXvLKctkxYITthuTSlFVxllbPGVqqSqgMGRFWkoaxjh-h0vXcRw_8J0qgHlyz0vfEQpqRlXTGupPwWpIIJyZTKYLUGbQwpRej0IrrBxA9NiV4Fob-C0CuXdS31VxCa57mTzYGpGaDdTm2cz_rPjW6SNX0XjbcubbGSUs5pmbHfawyya-8Ook7WgbfQugh21G1w3zzyCdempOo</recordid><startdate>19970801</startdate><enddate>19970801</enddate><creator>Misao, Ryou</creator><creator>Fujimoto, Jiro</creator><creator>Nakanishi, Yoshihito</creator><creator>Tamaya, Teruhiko</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19970801</creationdate><title>Expression of sex hormone-binding globulin exon VII splicing variant mRNA in human uterine endometrium</title><author>Misao, Ryou ; Fujimoto, Jiro ; Nakanishi, Yoshihito ; Tamaya, Teruhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-ac1b50d8420daab74245c41d36c67f6c47a2c6480da43bc58578fe3e0850b13f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adult</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Biological and medical sciences</topic><topic>DNA Primers - genetics</topic><topic>Endometrium - metabolism</topic><topic>Exons</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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| ispartof | The Journal of steroid biochemistry and molecular biology, 1997-08, Vol.62 (5), p.385-390 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Base Sequence Binding Sites - genetics Biological and medical sciences DNA Primers - genetics Endometrium - metabolism Exons Female Fundamental and applied biological sciences. Psychology Gene Expression Genetic Variation Humans In Vitro Techniques Mammalian female genital system Morphology. Physiology Polymerase Chain Reaction RNA Splicing RNA, Messenger - genetics RNA, Messenger - metabolism Sex Hormone-Binding Globulin - genetics Sex Hormone-Binding Globulin - metabolism Vertebrates: reproduction |
| title | Expression of sex hormone-binding globulin exon VII splicing variant mRNA in human uterine endometrium |
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