Histamine-induced Calcium Released from Cultured Human Mucosal Microvascular Endothelial Cells from Nasal Inferior Turbinate
Changes in cytosolic Ca2+ concentration ([Ca2+],) in cultured human mucosal microvascular endothelial cells (HM-MECs) from nasal inferior turbinate were measured using a fluorescent Ca2+-sensitive dye, fura-2, and photometric fluorescence microscopy. Histamine caused a transient increase in intracel...
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| Veröffentlicht in: | Acta oto-laryngologica 1997, Vol.117 (6), p.864-870 |
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| container_title | Acta oto-laryngologica |
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| creator | Ikeda, Hiroki Kubo, Nobuo Nakamura, Akihiko Harada, Narinobu Minamino, Masayuki Yamashita, Toshio |
| description | Changes in cytosolic Ca2+ concentration ([Ca2+],) in cultured human mucosal microvascular endothelial cells (HM-MECs) from nasal inferior turbinate were measured using a fluorescent Ca2+-sensitive dye, fura-2, and photometric fluorescence microscopy. Histamine caused a transient increase in intracellular free Ca2+ in cell populations and in individual cells, followed by a decrease to a sustained elevation. Histamine (100 μ) elevated [Ca3+]i in HMMECs up to 563 ± 20 nM from a resting level of 60 ± 45 nM (means ± SD, n = 31). Promethazine (a histamine H, receptor antagonist) inhibited [Ca2+] increase during histamine stimulation, whereas cimetidine (a H2 receptor antagonist) and thioperamide (a H, receptor antagonist) showed no inhibition. These results suggest that the histamine increase [Ca2+]i in HMMECs induces both a Ca2+ release from stores and a Ca2+ influx through activation of the H, receptor. |
| doi_str_mv | 10.3109/00016489709114216 |
| format | Article |
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Histamine caused a transient increase in intracellular free Ca2+ in cell populations and in individual cells, followed by a decrease to a sustained elevation. Histamine (100 μ) elevated [Ca3+]i in HMMECs up to 563 ± 20 nM from a resting level of 60 ± 45 nM (means ± SD, n = 31). Promethazine (a histamine H, receptor antagonist) inhibited [Ca2+] increase during histamine stimulation, whereas cimetidine (a H2 receptor antagonist) and thioperamide (a H, receptor antagonist) showed no inhibition. These results suggest that the histamine increase [Ca2+]i in HMMECs induces both a Ca2+ release from stores and a Ca2+ influx through activation of the H, receptor.</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.3109/00016489709114216</identifier><identifier>PMID: 9442829</identifier><identifier>CODEN: AOLAAJ</identifier><language>eng</language><publisher>Stockholm: Informa UK Ltd</publisher><subject>Biological and medical sciences ; Calcium - metabolism ; Cells, Cultured ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; fura-2 ; histamine ; Histamine - pharmacology ; Histamine Antagonists - pharmacology ; histamine receptor ; human mucosal microvascular endothelial cells ; Humans ; Medical sciences ; nasal allergy ; Nasal Mucosa - cytology ; Nasal Mucosa - drug effects ; Nasal Mucosa - metabolism ; Non tumoral diseases ; Otorhinolaryngology. Stomatology ; Receptors, Histamine - physiology ; Respiratory Hypersensitivity - physiopathology ; Turbinates ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology ; vascular permeability</subject><ispartof>Acta oto-laryngologica, 1997, Vol.117 (6), p.864-870</ispartof><rights>1997 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1997</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-8cabd1db6492014b9dd99fdaeb2208bdf730c8d719edbd1d57b883ea4b3c7cf13</citedby><cites>FETCH-LOGICAL-c430t-8cabd1db6492014b9dd99fdaeb2208bdf730c8d719edbd1d57b883ea4b3c7cf13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/00016489709114216$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/00016489709114216$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>315,781,785,4025,27928,27929,27930,59652,59758,60441,60547,61226,61261,61407,61442</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2060721$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9442829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikeda, Hiroki</creatorcontrib><creatorcontrib>Kubo, Nobuo</creatorcontrib><creatorcontrib>Nakamura, Akihiko</creatorcontrib><creatorcontrib>Harada, Narinobu</creatorcontrib><creatorcontrib>Minamino, Masayuki</creatorcontrib><creatorcontrib>Yamashita, Toshio</creatorcontrib><title>Histamine-induced Calcium Released from Cultured Human Mucosal Microvascular Endothelial Cells from Nasal Inferior Turbinate</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>Changes in cytosolic Ca2+ concentration ([Ca2+],) in cultured human mucosal microvascular endothelial cells (HM-MECs) from nasal inferior turbinate were measured using a fluorescent Ca2+-sensitive dye, fura-2, and photometric fluorescence microscopy. Histamine caused a transient increase in intracellular free Ca2+ in cell populations and in individual cells, followed by a decrease to a sustained elevation. Histamine (100 μ) elevated [Ca3+]i in HMMECs up to 563 ± 20 nM from a resting level of 60 ± 45 nM (means ± SD, n = 31). Promethazine (a histamine H, receptor antagonist) inhibited [Ca2+] increase during histamine stimulation, whereas cimetidine (a H2 receptor antagonist) and thioperamide (a H, receptor antagonist) showed no inhibition. These results suggest that the histamine increase [Ca2+]i in HMMECs induces both a Ca2+ release from stores and a Ca2+ influx through activation of the H, receptor.</description><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>fura-2</subject><subject>histamine</subject><subject>Histamine - pharmacology</subject><subject>Histamine Antagonists - pharmacology</subject><subject>histamine receptor</subject><subject>human mucosal microvascular endothelial cells</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>nasal allergy</subject><subject>Nasal Mucosa - cytology</subject><subject>Nasal Mucosa - drug effects</subject><subject>Nasal Mucosa - metabolism</subject><subject>Non tumoral diseases</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Receptors, Histamine - physiology</subject><subject>Respiratory Hypersensitivity - physiopathology</subject><subject>Turbinates</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><subject>vascular permeability</subject><issn>0001-6489</issn><issn>1651-2251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1rFTEUxYMo9bX6B7gQZiHuxiaZzEfQjQytr9BaKHU93HzxUjJJTSZKwT_eDO9ZkEJX4eb8zuXcg9A7gj81BPNTjDHp2MB7zAlhlHQv0IZ0LakpbclLtFn1egVeo-OU7taRD-0ROuKM0YHyDfqztWmB2XpdW6-y1KoawUmb5-pGOw2pfJgY5mrMbsmxTNs8g6-usgwJXHVlZQy_IMnsIFZnXoVlp50tyqidS3vvd1jRC290tCFWtzkK62HRb9ArAy7pt4f3BP04P7sdt_Xl9beL8etlLVmDl3qQIBRRomOcYsIEV4pzo0ALSvEglOkbLAfVE67VCra9GIZGAxON7KUhzQn6uN97H8PPrNMyzTbJkg-8DjlNPW8byvq2gGQPlqNSitpM99HOEB8mgqe18elJ48Xz_rA8i1mrR8eh4qJ_OOilJXAmgpc2PWIUd7ina8Yve8x6E-IMv0N0alrgwYX4z9M8l-Lzf_adBrfsJEQ93YUcfan3mRv-Ak1ZsDo</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Ikeda, Hiroki</creator><creator>Kubo, Nobuo</creator><creator>Nakamura, Akihiko</creator><creator>Harada, Narinobu</creator><creator>Minamino, Masayuki</creator><creator>Yamashita, Toshio</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>1997</creationdate><title>Histamine-induced Calcium Released from Cultured Human Mucosal Microvascular Endothelial Cells from Nasal Inferior Turbinate</title><author>Ikeda, Hiroki ; Kubo, Nobuo ; Nakamura, Akihiko ; Harada, Narinobu ; Minamino, Masayuki ; Yamashita, Toshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-8cabd1db6492014b9dd99fdaeb2208bdf730c8d719edbd1d57b883ea4b3c7cf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Cells, Cultured</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>fura-2</topic><topic>histamine</topic><topic>Histamine - pharmacology</topic><topic>Histamine Antagonists - pharmacology</topic><topic>histamine receptor</topic><topic>human mucosal microvascular endothelial cells</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>nasal allergy</topic><topic>Nasal Mucosa - cytology</topic><topic>Nasal Mucosa - drug effects</topic><topic>Nasal Mucosa - metabolism</topic><topic>Non tumoral diseases</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Receptors, Histamine - physiology</topic><topic>Respiratory Hypersensitivity - physiopathology</topic><topic>Turbinates</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><topic>vascular permeability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikeda, Hiroki</creatorcontrib><creatorcontrib>Kubo, Nobuo</creatorcontrib><creatorcontrib>Nakamura, Akihiko</creatorcontrib><creatorcontrib>Harada, Narinobu</creatorcontrib><creatorcontrib>Minamino, Masayuki</creatorcontrib><creatorcontrib>Yamashita, Toshio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Acta oto-laryngologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikeda, Hiroki</au><au>Kubo, Nobuo</au><au>Nakamura, Akihiko</au><au>Harada, Narinobu</au><au>Minamino, Masayuki</au><au>Yamashita, Toshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histamine-induced Calcium Released from Cultured Human Mucosal Microvascular Endothelial Cells from Nasal Inferior Turbinate</atitle><jtitle>Acta oto-laryngologica</jtitle><addtitle>Acta Otolaryngol</addtitle><date>1997</date><risdate>1997</risdate><volume>117</volume><issue>6</issue><spage>864</spage><epage>870</epage><pages>864-870</pages><issn>0001-6489</issn><eissn>1651-2251</eissn><coden>AOLAAJ</coden><abstract>Changes in cytosolic Ca2+ concentration ([Ca2+],) in cultured human mucosal microvascular endothelial cells (HM-MECs) from nasal inferior turbinate were measured using a fluorescent Ca2+-sensitive dye, fura-2, and photometric fluorescence microscopy. Histamine caused a transient increase in intracellular free Ca2+ in cell populations and in individual cells, followed by a decrease to a sustained elevation. Histamine (100 μ) elevated [Ca3+]i in HMMECs up to 563 ± 20 nM from a resting level of 60 ± 45 nM (means ± SD, n = 31). Promethazine (a histamine H, receptor antagonist) inhibited [Ca2+] increase during histamine stimulation, whereas cimetidine (a H2 receptor antagonist) and thioperamide (a H, receptor antagonist) showed no inhibition. These results suggest that the histamine increase [Ca2+]i in HMMECs induces both a Ca2+ release from stores and a Ca2+ influx through activation of the H, receptor.</abstract><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>9442829</pmid><doi>10.3109/00016489709114216</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Biological and medical sciences Calcium - metabolism Cells, Cultured Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism fura-2 histamine Histamine - pharmacology Histamine Antagonists - pharmacology histamine receptor human mucosal microvascular endothelial cells Humans Medical sciences nasal allergy Nasal Mucosa - cytology Nasal Mucosa - drug effects Nasal Mucosa - metabolism Non tumoral diseases Otorhinolaryngology. Stomatology Receptors, Histamine - physiology Respiratory Hypersensitivity - physiopathology Turbinates Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology vascular permeability |
| title | Histamine-induced Calcium Released from Cultured Human Mucosal Microvascular Endothelial Cells from Nasal Inferior Turbinate |
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