Purification of a novel insulin-stimulated protein kinase from rat liver

We previously described a novel insulin-stimulated protein kinase activity that phosphorylates Kemptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly) in cytosolic extracts of adipocytes (Yu, K-T., Khalaf, N., and Czech, M. P. (1987) J. Biol. Chem. 262, 16677-16685). In the present experiments, cytosolic extracts of...

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Veröffentlicht in:	The Journal of biological chemistry 1990-01, Vol.265 (1), p.227-234
Hauptverfasser:	KLARLUND, J. K, BRADFORD, A. P, MILLA, M. G, CZECH, M. P
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography Cytosol - enzymology Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology insulin Insulin - pharmacology Isoelectric Focusing liver Liver - enzymology Magnesium - pharmacology Male Manganese - pharmacology Molecular Weight Oligopeptides - metabolism Protein Kinases - isolation & purification Protein Kinases - metabolism Rats Rats, Inbred Strains Substrate Specificity Transferases
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