Spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates
Strains of vesicular stomatitis virus, New Jersey serotype (VSV-NJ), isolated from diseased cattle or swine were examined by genomic RNA sequencing for genetic diversity potentially leading to antigenic variations in their type-specific glycoproteins as determined by reactivity with epitope-specific...
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description | Strains of vesicular stomatitis virus, New Jersey serotype (VSV-NJ), isolated from diseased cattle or swine were examined by genomic RNA sequencing for genetic diversity potentially leading to antigenic variations in their type-specific glycoproteins as determined by reactivity with epitope-specific monoclonal antibodies (MAbs). Seven field isolates recovered in Colorado, New Mexico, Georgia, and Mexico during the widespread 1982–1985 epizootic in the western United States resembled the prototypic 1952 Hazelhurst subtype by partial sequence homology, but amino acid reversions to the 1949 Ogden subtype occurred frequently. When studies were performed with MAbs directed to the Ogden subtype glycoprotein, relatively limited antigenic variation, and only in neutralization epitope VIII, was noted among two of five epizootic isolates from Colorado and New Mexico. However, amino acid differences in the glycoprotein of a 1983 isolate from an enzootic region of Georgia resulted in major antigenic deficiencies in epitopes V, VI, and VII as determined by Western blotting and neutralization of infectivity with epitope-specific MAbs. Quite a few genetic but no antigenic differences were noted in an enzootic 1984 isolate from Mexico, a potential origin of the United States epizootic. Marked or complete loss of epitopes VII, VI, VIII, and V can be traced to spontaneous mutations leading to amino acid substitutions at glycoprotein positions 199, 263, 275, and 317, respectively, in the enzootic Georgia isolate 07/83-GA-P and the epizootic New Mexico isolate 06/85-NM-B. By comparison, closely adjacent amino acid substitutions at glycoprotein positions 210, 268, 277, and 364 occurred in epitope-deficient mutants selected for resistance to neutralization by MAbs specific for epitopes VII, VI, VIII, and V, respectively. Two neutralization epitopes designated X and XI were found to be unique for the G protein of the 1952 Hazelhurst isolate … /52-GA-P. The epitope X-specific MAb H21, in particular, failed to neutralize the infectivity not only of the Ogden subtype …/49-UT-B but also was ineffective against all the 1982–1985 field isolates. The classical 1952 Hazelhurst strain of VSV-NJ is genetically and antigenically quite different from those viruses isolated during the 1982–1985 epizootic. |
doi_str_mv | 10.1016/0042-6822(90)90055-V |
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Seven field isolates recovered in Colorado, New Mexico, Georgia, and Mexico during the widespread 1982–1985 epizootic in the western United States resembled the prototypic 1952 Hazelhurst subtype by partial sequence homology, but amino acid reversions to the 1949 Ogden subtype occurred frequently. When studies were performed with MAbs directed to the Ogden subtype glycoprotein, relatively limited antigenic variation, and only in neutralization epitope VIII, was noted among two of five epizootic isolates from Colorado and New Mexico. However, amino acid differences in the glycoprotein of a 1983 isolate from an enzootic region of Georgia resulted in major antigenic deficiencies in epitopes V, VI, and VII as determined by Western blotting and neutralization of infectivity with epitope-specific MAbs. Quite a few genetic but no antigenic differences were noted in an enzootic 1984 isolate from Mexico, a potential origin of the United States epizootic. Marked or complete loss of epitopes VII, VI, VIII, and V can be traced to spontaneous mutations leading to amino acid substitutions at glycoprotein positions 199, 263, 275, and 317, respectively, in the enzootic Georgia isolate 07/83-GA-P and the epizootic New Mexico isolate 06/85-NM-B. By comparison, closely adjacent amino acid substitutions at glycoprotein positions 210, 268, 277, and 364 occurred in epitope-deficient mutants selected for resistance to neutralization by MAbs specific for epitopes VII, VI, VIII, and V, respectively. Two neutralization epitopes designated X and XI were found to be unique for the G protein of the 1952 Hazelhurst isolate … /52-GA-P. The epitope X-specific MAb H21, in particular, failed to neutralize the infectivity not only of the Ogden subtype …/49-UT-B but also was ineffective against all the 1982–1985 field isolates. The classical 1952 Hazelhurst strain of VSV-NJ is genetically and antigenically quite different from those viruses isolated during the 1982–1985 epizootic.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(90)90055-V</identifier><identifier>PMID: 1688475</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Antigenic Variation - genetics ; Antigens, Viral - genetics ; Base Sequence ; Biological and medical sciences ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - microbiology ; Disease Outbreaks - veterinary ; Epitopes - genetics ; Fundamental and applied biological sciences. Psychology ; Membrane Glycoproteins ; Mexico - epidemiology ; Microbiology ; Molecular Sequence Data ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; RNA, Viral - genetics ; Stomatitis - epidemiology ; Stomatitis - microbiology ; Stomatitis - veterinary ; Swine ; Swine Diseases - epidemiology ; Swine Diseases - microbiology ; United States - epidemiology ; Vesiculovirus - genetics ; Vesiculovirus - immunology ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Virology ; Virus Diseases - epidemiology ; Virus Diseases - microbiology ; Virus Diseases - veterinary</subject><ispartof>Virology (New York, N.Y.), 1990, Vol.174 (1), p.70-78</ispartof><rights>1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-b431ecf3d5d67d948f243bf47fde41768bd90c97f288b7644181058f7c94ae053</citedby><cites>FETCH-LOGICAL-c417t-b431ecf3d5d67d948f243bf47fde41768bd90c97f288b7644181058f7c94ae053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(90)90055-V$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6676629$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1688475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Lizhong</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Snyder, Ruth M.</creatorcontrib><creatorcontrib>Wagner, Robert R.</creatorcontrib><title>Spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Strains of vesicular stomatitis virus, New Jersey serotype (VSV-NJ), isolated from diseased cattle or swine were examined by genomic RNA sequencing for genetic diversity potentially leading to antigenic variations in their type-specific glycoproteins as determined by reactivity with epitope-specific monoclonal antibodies (MAbs). Seven field isolates recovered in Colorado, New Mexico, Georgia, and Mexico during the widespread 1982–1985 epizootic in the western United States resembled the prototypic 1952 Hazelhurst subtype by partial sequence homology, but amino acid reversions to the 1949 Ogden subtype occurred frequently. When studies were performed with MAbs directed to the Ogden subtype glycoprotein, relatively limited antigenic variation, and only in neutralization epitope VIII, was noted among two of five epizootic isolates from Colorado and New Mexico. However, amino acid differences in the glycoprotein of a 1983 isolate from an enzootic region of Georgia resulted in major antigenic deficiencies in epitopes V, VI, and VII as determined by Western blotting and neutralization of infectivity with epitope-specific MAbs. Quite a few genetic but no antigenic differences were noted in an enzootic 1984 isolate from Mexico, a potential origin of the United States epizootic. Marked or complete loss of epitopes VII, VI, VIII, and V can be traced to spontaneous mutations leading to amino acid substitutions at glycoprotein positions 199, 263, 275, and 317, respectively, in the enzootic Georgia isolate 07/83-GA-P and the epizootic New Mexico isolate 06/85-NM-B. By comparison, closely adjacent amino acid substitutions at glycoprotein positions 210, 268, 277, and 364 occurred in epitope-deficient mutants selected for resistance to neutralization by MAbs specific for epitopes VII, VI, VIII, and V, respectively. Two neutralization epitopes designated X and XI were found to be unique for the G protein of the 1952 Hazelhurst isolate … /52-GA-P. The epitope X-specific MAb H21, in particular, failed to neutralize the infectivity not only of the Ogden subtype …/49-UT-B but also was ineffective against all the 1982–1985 field isolates. The classical 1952 Hazelhurst strain of VSV-NJ is genetically and antigenically quite different from those viruses isolated during the 1982–1985 epizootic.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antigenic Variation - genetics</subject><subject>Antigens, Viral - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - microbiology</subject><subject>Disease Outbreaks - veterinary</subject><subject>Epitopes - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Membrane Glycoproteins</subject><subject>Mexico - epidemiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>RNA, Viral - genetics</subject><subject>Stomatitis - epidemiology</subject><subject>Stomatitis - microbiology</subject><subject>Stomatitis - veterinary</subject><subject>Swine</subject><subject>Swine Diseases - epidemiology</subject><subject>Swine Diseases - microbiology</subject><subject>United States - epidemiology</subject><subject>Vesiculovirus - genetics</subject><subject>Vesiculovirus - immunology</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><subject>Virus Diseases - epidemiology</subject><subject>Virus Diseases - microbiology</subject><subject>Virus Diseases - veterinary</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo6-zqN1DIQWQ9tCbd6aRzWZDFf7DgQd1rSCeVsaSnMybpkf32ZuxhvSk5hFT96lF5j5BnnL3mjMs3jIm2kUPbXmr2SjPW983tA7LhTMuGdYI_JJt75DE5z_kHq2-l2Bk543IYhOo35NeXfZyLnSEume6WYgvGOdMJrMd5S0ukdi64hRkdPdiEpz7OtHwHup3uXNynWABrMQZ6gIxumWyiucRdhQtmesBUxQPC5CnmONkC-Ql5FOyU4enpviDf3r_7ev2xufn84dP125vGCa5KM4qOgwud771UXoshtKIbg1DBQwXkMHrNnFahHYZRSSH4wFk_BOW0sMD67oK8XHXrlj8XyMXsMDuYpvXLRum-46xr_wvyXtTTqQqKFXQp5pwgmH3CnU13hjNzDMYcXTdH141m5k8w5raOPT_pL-MO_N-hNYnaf3Hq2-zsFJKdHeZ7TEolZasrdrViUE07ICSTHcLswGMCV4yP-O89fgMKxKxV</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Luo, Lizhong</creator><creator>Li, Yan</creator><creator>Snyder, Ruth M.</creator><creator>Wagner, Robert R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates</title><author>Luo, Lizhong ; Li, Yan ; Snyder, Ruth M. ; Wagner, Robert R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b431ecf3d5d67d948f243bf47fde41768bd90c97f288b7644181058f7c94ae053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antigenic Variation - genetics</topic><topic>Antigens, Viral - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - microbiology</topic><topic>Disease Outbreaks - veterinary</topic><topic>Epitopes - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Membrane Glycoproteins</topic><topic>Mexico - epidemiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>RNA, Viral - genetics</topic><topic>Stomatitis - epidemiology</topic><topic>Stomatitis - microbiology</topic><topic>Stomatitis - veterinary</topic><topic>Swine</topic><topic>Swine Diseases - epidemiology</topic><topic>Swine Diseases - microbiology</topic><topic>United States - epidemiology</topic><topic>Vesiculovirus - genetics</topic><topic>Vesiculovirus - immunology</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><topic>Virus Diseases - epidemiology</topic><topic>Virus Diseases - microbiology</topic><topic>Virus Diseases - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Lizhong</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Snyder, Ruth M.</creatorcontrib><creatorcontrib>Wagner, Robert R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Lizhong</au><au>Li, Yan</au><au>Snyder, Ruth M.</au><au>Wagner, Robert R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1990</date><risdate>1990</risdate><volume>174</volume><issue>1</issue><spage>70</spage><epage>78</epage><pages>70-78</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>Strains of vesicular stomatitis virus, New Jersey serotype (VSV-NJ), isolated from diseased cattle or swine were examined by genomic RNA sequencing for genetic diversity potentially leading to antigenic variations in their type-specific glycoproteins as determined by reactivity with epitope-specific monoclonal antibodies (MAbs). Seven field isolates recovered in Colorado, New Mexico, Georgia, and Mexico during the widespread 1982–1985 epizootic in the western United States resembled the prototypic 1952 Hazelhurst subtype by partial sequence homology, but amino acid reversions to the 1949 Ogden subtype occurred frequently. When studies were performed with MAbs directed to the Ogden subtype glycoprotein, relatively limited antigenic variation, and only in neutralization epitope VIII, was noted among two of five epizootic isolates from Colorado and New Mexico. However, amino acid differences in the glycoprotein of a 1983 isolate from an enzootic region of Georgia resulted in major antigenic deficiencies in epitopes V, VI, and VII as determined by Western blotting and neutralization of infectivity with epitope-specific MAbs. Quite a few genetic but no antigenic differences were noted in an enzootic 1984 isolate from Mexico, a potential origin of the United States epizootic. Marked or complete loss of epitopes VII, VI, VIII, and V can be traced to spontaneous mutations leading to amino acid substitutions at glycoprotein positions 199, 263, 275, and 317, respectively, in the enzootic Georgia isolate 07/83-GA-P and the epizootic New Mexico isolate 06/85-NM-B. By comparison, closely adjacent amino acid substitutions at glycoprotein positions 210, 268, 277, and 364 occurred in epitope-deficient mutants selected for resistance to neutralization by MAbs specific for epitopes VII, VI, VIII, and V, respectively. Two neutralization epitopes designated X and XI were found to be unique for the G protein of the 1952 Hazelhurst isolate … /52-GA-P. The epitope X-specific MAb H21, in particular, failed to neutralize the infectivity not only of the Ogden subtype …/49-UT-B but also was ineffective against all the 1982–1985 field isolates. The classical 1952 Hazelhurst strain of VSV-NJ is genetically and antigenically quite different from those viruses isolated during the 1982–1985 epizootic.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1688475</pmid><doi>10.1016/0042-6822(90)90055-V</doi><tpages>9</tpages></addata></record> |
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subjects | Amino Acid Sequence Animals Antigenic Variation - genetics Antigens, Viral - genetics Base Sequence Biological and medical sciences Cattle Cattle Diseases - epidemiology Cattle Diseases - microbiology Disease Outbreaks - veterinary Epitopes - genetics Fundamental and applied biological sciences. Psychology Membrane Glycoproteins Mexico - epidemiology Microbiology Molecular Sequence Data Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA, Viral - genetics Stomatitis - epidemiology Stomatitis - microbiology Stomatitis - veterinary Swine Swine Diseases - epidemiology Swine Diseases - microbiology United States - epidemiology Vesiculovirus - genetics Vesiculovirus - immunology Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Virology Virus Diseases - epidemiology Virus Diseases - microbiology Virus Diseases - veterinary |
title | Spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates |
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