Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase
The polyketide synthase gene pksM was detected in the genomic DNA library of Aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-MSAS) gene of Penicillium patulum as a probe. 9524 bp of the cloned DNA were sequenced and a 5.5 kb open reading frame was revealed. A single...
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| Veröffentlicht in: | Folia microbiologica 1997, Vol.42 (5), p.419-430 |
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| creator | PAZOUTOVA, S LINKA, M STORKOVA, S SCHWAB, H |
| description | The polyketide synthase gene pksM was detected in the genomic DNA library of Aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-MSAS) gene of Penicillium patulum as a probe. 9524 bp of the cloned DNA were sequenced and a 5.5 kb open reading frame was revealed. A single intron (62 bp) was identified in the conserved position. Two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation initiation codon ATG. The conserved active site motifs for ketosynthase, acyltransferase, dehydratase, ketoreductase and acyl carrier protein were found within the predicted polypeptide consisting of 1803 amino acids. Unlike the P. patulum 6-MSAS gene, the transcription of pksM from A. terreus was observed in the middle of the vegetative growth phase. |
| doi_str_mv | 10.1007/BF02826548 |
| format | Article |
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A single intron (62 bp) was identified in the conserved position. Two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation initiation codon ATG. The conserved active site motifs for ketosynthase, acyltransferase, dehydratase, ketoreductase and acyl carrier protein were found within the predicted polypeptide consisting of 1803 amino acids. Unlike the P. patulum 6-MSAS gene, the transcription of pksM from A. terreus was observed in the middle of the vegetative growth phase.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/BF02826548</identifier><identifier>PMID: 9438344</identifier><identifier>CODEN: FOMIAZ</identifier><language>eng</language><publisher>Praha: Academia</publisher><subject>3-Oxoacyl-(Acyl-Carrier-Protein) Reductase ; 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - genetics ; Acyl Carrier Protein - genetics ; Acyltransferases - genetics ; Alcohol Oxidoreductases - genetics ; Amino Acid Sequence ; Aspergillus - genetics ; Aspergillus - growth & development ; Aspergillus - metabolism ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Cloning, Molecular ; Codon, Initiator ; DNA Probes ; DNA, Fungal - analysis ; DNA, Fungal - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Library ; Genes, Fungal ; Genetics ; Hydro-Lyases - genetics ; Introns ; Ligases - genetics ; Microbiology ; Molecular Sequence Data ; Multienzyme Complexes - genetics ; Multienzyme Complexes - metabolism ; Nucleic Acid Hybridization ; Open Reading Frames ; Oxidoreductases - genetics ; Penicillium - genetics ; Plasmids ; Polymerase Chain Reaction ; Restriction Mapping ; RNA, Fungal - isolation & purification ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Transcription, Genetic</subject><ispartof>Folia microbiologica, 1997, Vol.42 (5), p.419-430</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,4022,4048,4049,23928,23929,25138,27921,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2097642$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9438344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PAZOUTOVA, S</creatorcontrib><creatorcontrib>LINKA, M</creatorcontrib><creatorcontrib>STORKOVA, S</creatorcontrib><creatorcontrib>SCHWAB, H</creatorcontrib><title>Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase</title><title>Folia microbiologica</title><addtitle>Folia Microbiol (Praha)</addtitle><description>The polyketide synthase gene pksM was detected in the genomic DNA library of Aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-MSAS) gene of Penicillium patulum as a probe. 9524 bp of the cloned DNA were sequenced and a 5.5 kb open reading frame was revealed. A single intron (62 bp) was identified in the conserved position. Two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation initiation codon ATG. The conserved active site motifs for ketosynthase, acyltransferase, dehydratase, ketoreductase and acyl carrier protein were found within the predicted polypeptide consisting of 1803 amino acids. Unlike the P. patulum 6-MSAS gene, the transcription of pksM from A. terreus was observed in the middle of the vegetative growth phase.</description><subject>3-Oxoacyl-(Acyl-Carrier-Protein) Reductase</subject><subject>3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - genetics</subject><subject>Acyl Carrier Protein - genetics</subject><subject>Acyltransferases - genetics</subject><subject>Alcohol Oxidoreductases - genetics</subject><subject>Amino Acid Sequence</subject><subject>Aspergillus - genetics</subject><subject>Aspergillus - growth & development</subject><subject>Aspergillus - metabolism</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cloning, Molecular</subject><subject>Codon, Initiator</subject><subject>DNA Probes</subject><subject>DNA, Fungal - analysis</subject><subject>DNA, Fungal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Library</subject><subject>Genes, Fungal</subject><subject>Genetics</subject><subject>Hydro-Lyases - genetics</subject><subject>Introns</subject><subject>Ligases - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multienzyme Complexes - genetics</subject><subject>Multienzyme Complexes - metabolism</subject><subject>Nucleic Acid Hybridization</subject><subject>Open Reading Frames</subject><subject>Oxidoreductases - genetics</subject><subject>Penicillium - genetics</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Restriction Mapping</subject><subject>RNA, Fungal - isolation & purification</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic</subject><issn>0015-5632</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtOw0AUBVcIFEKgoUfaAtEZ9m1vGSICSEFQpLfW9rVj4hd7bUH-HiMsWqpTzGiKQ8glZ7ecsfDufs1EJIxW0RGZ8yhUgZXaHJM5Y1wH2khxSs4Q3xkzTEkxIzOrZCSVmpPtW1sd9tCXGVA8NP3OIdACGqDdHl9o7tuaLrEDX5RVNSDtwXsYF746D4iQ0WzwZVPQwref_Y52P4FzcpK7CuFi2gXZrh-2q6dg8_r4vFpugk4Y0wc2YlZakWfSgo6E44nMjI00E2LkPFRprqxLMums0gm3NgcZcqa5USoBJhfk5jfb-fZjAOzjusQUqso10A4Yh1aLUVX_ityMX2j1U7yaxCGpIYs7X9bOH-LprpFfT9xh6qrcuyYt8U8TzIZGCfkNLCB3fg</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>PAZOUTOVA, S</creator><creator>LINKA, M</creator><creator>STORKOVA, S</creator><creator>SCHWAB, H</creator><general>Academia</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase</title><author>PAZOUTOVA, S ; LINKA, M ; STORKOVA, S ; SCHWAB, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-9809392fd39e582a1b3d6985022266174cf49abd3a945b199fe371051644be03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>3-Oxoacyl-(Acyl-Carrier-Protein) Reductase</topic><topic>3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - genetics</topic><topic>Acyl Carrier Protein - genetics</topic><topic>Acyltransferases - genetics</topic><topic>Alcohol Oxidoreductases - genetics</topic><topic>Amino Acid Sequence</topic><topic>Aspergillus - genetics</topic><topic>Aspergillus - growth & development</topic><topic>Aspergillus - metabolism</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cloning, Molecular</topic><topic>Codon, Initiator</topic><topic>DNA Probes</topic><topic>DNA, Fungal - analysis</topic><topic>DNA, Fungal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Library</topic><topic>Genes, Fungal</topic><topic>Genetics</topic><topic>Hydro-Lyases - genetics</topic><topic>Introns</topic><topic>Ligases - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multienzyme Complexes - genetics</topic><topic>Multienzyme Complexes - metabolism</topic><topic>Nucleic Acid Hybridization</topic><topic>Open Reading Frames</topic><topic>Oxidoreductases - genetics</topic><topic>Penicillium - genetics</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Restriction Mapping</topic><topic>RNA, Fungal - isolation & purification</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PAZOUTOVA, S</creatorcontrib><creatorcontrib>LINKA, M</creatorcontrib><creatorcontrib>STORKOVA, S</creatorcontrib><creatorcontrib>SCHWAB, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PAZOUTOVA, S</au><au>LINKA, M</au><au>STORKOVA, S</au><au>SCHWAB, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase</atitle><jtitle>Folia microbiologica</jtitle><addtitle>Folia Microbiol (Praha)</addtitle><date>1997</date><risdate>1997</risdate><volume>42</volume><issue>5</issue><spage>419</spage><epage>430</epage><pages>419-430</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><coden>FOMIAZ</coden><abstract>The polyketide synthase gene pksM was detected in the genomic DNA library of Aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-MSAS) gene of Penicillium patulum as a probe. 9524 bp of the cloned DNA were sequenced and a 5.5 kb open reading frame was revealed. A single intron (62 bp) was identified in the conserved position. Two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation initiation codon ATG. The conserved active site motifs for ketosynthase, acyltransferase, dehydratase, ketoreductase and acyl carrier protein were found within the predicted polypeptide consisting of 1803 amino acids. Unlike the P. patulum 6-MSAS gene, the transcription of pksM from A. terreus was observed in the middle of the vegetative growth phase.</abstract><cop>Praha</cop><pub>Academia</pub><pmid>9438344</pmid><doi>10.1007/BF02826548</doi><tpages>12</tpages></addata></record> |
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| ispartof | Folia microbiologica, 1997, Vol.42 (5), p.419-430 |
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| subjects | 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase - genetics Acyl Carrier Protein - genetics Acyltransferases - genetics Alcohol Oxidoreductases - genetics Amino Acid Sequence Aspergillus - genetics Aspergillus - growth & development Aspergillus - metabolism Bacteriology Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular Codon, Initiator DNA Probes DNA, Fungal - analysis DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Gene Expression Gene Library Genes, Fungal Genetics Hydro-Lyases - genetics Introns Ligases - genetics Microbiology Molecular Sequence Data Multienzyme Complexes - genetics Multienzyme Complexes - metabolism Nucleic Acid Hybridization Open Reading Frames Oxidoreductases - genetics Penicillium - genetics Plasmids Polymerase Chain Reaction Restriction Mapping RNA, Fungal - isolation & purification Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic |
| title | Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase |
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