Effect of SERCA Pump Inhibitors on Chemoresponses in Paramecium
Inhibitors of SERCA (sarcoplasmic/endoplasmic reticulum Ca2+‐dependent ATPase) calcium pumps were used to investigate the involvement of internal Ca2+ stores in the GTP response in Paramecium. External application of these inhibitors was found to dramatically alter the typical behavioral and electro...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 1997-11, Vol.44 (6), p.574-581 |
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| container_title | The Journal of eukaryotic microbiology |
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| creator | WASSENBERG, JAMES J. CLARK, KEVIN D. NELSON, DAVID L. |
| description | Inhibitors of SERCA (sarcoplasmic/endoplasmic reticulum Ca2+‐dependent ATPase) calcium pumps were used to investigate the involvement of internal Ca2+ stores in the GTP response in Paramecium. External application of these inhibitors was found to dramatically alter the typical behavioral and electrophysiological responses of Paramecium to extracellular chemical stimulation. In particular, 2.5‐di‐tert‐butylhydroquinone (BHQ) strongly inhibited the backward swimming response of paramecia to externally applied GTP, though it did not inhibit the associated whirling response. BHQ also prolonged the normally brief electrophysiological response of these cells to GTP. BHQ completely blocked the behavioral and electrophysiological responses of Paramecium to extracellular Ba2+, but had no measurable effect on the behavioral or electrophysiological responses of these cells to another depolarizing stimulus, elevated external K+ concentration. These results suggest the involvement of nonciliary Ca2+ ions in the GTP and Ba2+ responses. |
| doi_str_mv | 10.1111/j.1550-7408.1997.tb05962.x |
| format | Article |
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External application of these inhibitors was found to dramatically alter the typical behavioral and electrophysiological responses of Paramecium to extracellular chemical stimulation. In particular, 2.5‐di‐tert‐butylhydroquinone (BHQ) strongly inhibited the backward swimming response of paramecia to externally applied GTP, though it did not inhibit the associated whirling response. BHQ also prolonged the normally brief electrophysiological response of these cells to GTP. BHQ completely blocked the behavioral and electrophysiological responses of Paramecium to extracellular Ba2+, but had no measurable effect on the behavioral or electrophysiological responses of these cells to another depolarizing stimulus, elevated external K+ concentration. These results suggest the involvement of nonciliary Ca2+ ions in the GTP and Ba2+ responses.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/j.1550-7408.1997.tb05962.x</identifier><identifier>PMID: 9435128</identifier><identifier>CODEN: JEMIED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Barium dance ; BHQ ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; calcium ; Calcium - metabolism ; Calcium-Transporting ATPases - antagonists & inhibitors ; Chemotactic Factors - pharmacology ; Chemotaxis - physiology ; electrophysiology ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; GTP ; Guanosine Triphosphate - pharmacology ; Hydroquinones - pharmacology ; Membrane Potentials ; Models, Biological ; Paramecium ; Paramecium tetraurelia - cytology ; Paramecium tetraurelia - drug effects ; Paramecium tetraurelia - enzymology ; Paramecium tetraurelia - physiology ; Protozoa ; thapsigargin</subject><ispartof>The Journal of eukaryotic microbiology, 1997-11, Vol.44 (6), p.574-581</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4674-500d154814a248a1f92b62568e59d5dee51b2285c50345ba0955a5467f0ccd003</citedby><cites>FETCH-LOGICAL-c4674-500d154814a248a1f92b62568e59d5dee51b2285c50345ba0955a5467f0ccd003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1550-7408.1997.tb05962.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1550-7408.1997.tb05962.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,1416,23928,23929,25138,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2125683$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9435128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WASSENBERG, JAMES J.</creatorcontrib><creatorcontrib>CLARK, KEVIN D.</creatorcontrib><creatorcontrib>NELSON, DAVID L.</creatorcontrib><title>Effect of SERCA Pump Inhibitors on Chemoresponses in Paramecium</title><title>The Journal of eukaryotic microbiology</title><addtitle>J Eukaryot Microbiol</addtitle><description>Inhibitors of SERCA (sarcoplasmic/endoplasmic reticulum Ca2+‐dependent ATPase) calcium pumps were used to investigate the involvement of internal Ca2+ stores in the GTP response in Paramecium. External application of these inhibitors was found to dramatically alter the typical behavioral and electrophysiological responses of Paramecium to extracellular chemical stimulation. In particular, 2.5‐di‐tert‐butylhydroquinone (BHQ) strongly inhibited the backward swimming response of paramecia to externally applied GTP, though it did not inhibit the associated whirling response. BHQ also prolonged the normally brief electrophysiological response of these cells to GTP. BHQ completely blocked the behavioral and electrophysiological responses of Paramecium to extracellular Ba2+, but had no measurable effect on the behavioral or electrophysiological responses of these cells to another depolarizing stimulus, elevated external K+ concentration. These results suggest the involvement of nonciliary Ca2+ ions in the GTP and Ba2+ responses.</description><subject>Animals</subject><subject>Barium dance</subject><subject>BHQ</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>Calcium-Transporting ATPases - antagonists & inhibitors</subject><subject>Chemotactic Factors - pharmacology</subject><subject>Chemotaxis - physiology</subject><subject>electrophysiology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GTP</subject><subject>Guanosine Triphosphate - pharmacology</subject><subject>Hydroquinones - pharmacology</subject><subject>Membrane Potentials</subject><subject>Models, Biological</subject><subject>Paramecium</subject><subject>Paramecium tetraurelia - cytology</subject><subject>Paramecium tetraurelia - drug effects</subject><subject>Paramecium tetraurelia - enzymology</subject><subject>Paramecium tetraurelia - physiology</subject><subject>Protozoa</subject><subject>thapsigargin</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEtLxDAYRYMovn-CUETctSZpvrZxoQzj-GLwLboLaZpixj7GpMXx39syZbZiNgnc890kB6FDggPSrZNZQACwHzOcBITzOGhSDDyiwWINba-i9e6Mo8gHGrIttOPcDGMSUUI20SZnIRCabKPzSZ5r1Xh17j1PnsYj76Et595N9WFS09TWeXXljT90WVvt5nXltPNM5T1IK0utTFvuoY1cFk7vD_suer2cvIyv_en91c14NPUVi2LmA8YZAZYQJilLJMk5TSMKUaKBZ5BpDSSlNAEFOGSQSswBJHSjOVYqwzjcRcfL3rmtv1rtGlEap3RRyErXrRMxB5JQxv8ESUwTHjLagadLUNnaOatzMbemlPZHECx6zWImepeidyl6zWLQLBbd8MFwS5uWOluNDl67_GjIpVOyyK2slHErjJL-72GHnS2xb1Pon388QNxOXiFmXYG_LDCu0YtVgbSfIorDGMTb3ZWY8uuLN3b7Lh7DX1U0pjo</recordid><startdate>199711</startdate><enddate>199711</enddate><creator>WASSENBERG, JAMES J.</creator><creator>CLARK, KEVIN D.</creator><creator>NELSON, DAVID L.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199711</creationdate><title>Effect of SERCA Pump Inhibitors on Chemoresponses in Paramecium</title><author>WASSENBERG, JAMES J. ; CLARK, KEVIN D. ; NELSON, DAVID L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4674-500d154814a248a1f92b62568e59d5dee51b2285c50345ba0955a5467f0ccd003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Barium dance</topic><topic>BHQ</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>Calcium-Transporting ATPases - antagonists & inhibitors</topic><topic>Chemotactic Factors - pharmacology</topic><topic>Chemotaxis - physiology</topic><topic>electrophysiology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GTP</topic><topic>Guanosine Triphosphate - pharmacology</topic><topic>Hydroquinones - pharmacology</topic><topic>Membrane Potentials</topic><topic>Models, Biological</topic><topic>Paramecium</topic><topic>Paramecium tetraurelia - cytology</topic><topic>Paramecium tetraurelia - drug effects</topic><topic>Paramecium tetraurelia - enzymology</topic><topic>Paramecium tetraurelia - physiology</topic><topic>Protozoa</topic><topic>thapsigargin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WASSENBERG, JAMES J.</creatorcontrib><creatorcontrib>CLARK, KEVIN D.</creatorcontrib><creatorcontrib>NELSON, DAVID L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WASSENBERG, JAMES J.</au><au>CLARK, KEVIN D.</au><au>NELSON, DAVID L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of SERCA Pump Inhibitors on Chemoresponses in Paramecium</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>1997-11</date><risdate>1997</risdate><volume>44</volume><issue>6</issue><spage>574</spage><epage>581</epage><pages>574-581</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><coden>JEMIED</coden><abstract>Inhibitors of SERCA (sarcoplasmic/endoplasmic reticulum Ca2+‐dependent ATPase) calcium pumps were used to investigate the involvement of internal Ca2+ stores in the GTP response in Paramecium. External application of these inhibitors was found to dramatically alter the typical behavioral and electrophysiological responses of Paramecium to extracellular chemical stimulation. In particular, 2.5‐di‐tert‐butylhydroquinone (BHQ) strongly inhibited the backward swimming response of paramecia to externally applied GTP, though it did not inhibit the associated whirling response. BHQ also prolonged the normally brief electrophysiological response of these cells to GTP. BHQ completely blocked the behavioral and electrophysiological responses of Paramecium to extracellular Ba2+, but had no measurable effect on the behavioral or electrophysiological responses of these cells to another depolarizing stimulus, elevated external K+ concentration. These results suggest the involvement of nonciliary Ca2+ ions in the GTP and Ba2+ responses.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9435128</pmid><doi>10.1111/j.1550-7408.1997.tb05962.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Barium dance BHQ Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences calcium Calcium - metabolism Calcium-Transporting ATPases - antagonists & inhibitors Chemotactic Factors - pharmacology Chemotaxis - physiology electrophysiology Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology GTP Guanosine Triphosphate - pharmacology Hydroquinones - pharmacology Membrane Potentials Models, Biological Paramecium Paramecium tetraurelia - cytology Paramecium tetraurelia - drug effects Paramecium tetraurelia - enzymology Paramecium tetraurelia - physiology Protozoa thapsigargin |
| title | Effect of SERCA Pump Inhibitors on Chemoresponses in Paramecium |
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