ISRf1, a transposable insertion sequence from Sinorhizobium fredii
Sinorhizobium fredii strain HH103, a nitrogen-fixing bacterial symbiont of plants, contains an insertion sequence (IS) that can transpose into plasmid pMUS248 and activate a promoterless TcR gene that is normally not expressed. We have cloned and characterized this element, which we designate ISRf1....
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| Veröffentlicht in: | Gene 1997-12, Vol.204 (1-2), p.63-69 |
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| container_issue | 1-2 |
| container_start_page | 63 |
| container_title | Gene |
| container_volume | 204 |
| creator | Vinardell, J M Ollero, F J Krishnan, H B del Rosario Espuny, M Villalobo, E Pueppke, S G Ruiz-Sainz, J E |
| description | Sinorhizobium fredii strain HH103, a nitrogen-fixing bacterial symbiont of plants, contains an insertion sequence (IS) that can transpose into plasmid pMUS248 and activate a promoterless TcR gene that is normally not expressed. We have cloned and characterized this element, which we designate ISRf1. The IS is 1002 bp in length, contains a single 513-bp open reading frame (ORF), is flanked by imperfect 36-bp terminal inverted repeats, and creates 5-bp target duplications. Two copies of ISRf1 are present in the genome of HH103, but it is absent from 12 other Sinorhizobium and Rhizobium strains. The element transposes at a frequency of 2.7 x 10(-6) per generation per cell. |
| doi_str_mv | 10.1016/S0378-1119(97)00523-4 |
| format | Article |
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We have cloned and characterized this element, which we designate ISRf1. The IS is 1002 bp in length, contains a single 513-bp open reading frame (ORF), is flanked by imperfect 36-bp terminal inverted repeats, and creates 5-bp target duplications. Two copies of ISRf1 are present in the genome of HH103, but it is absent from 12 other Sinorhizobium and Rhizobium strains. 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We have cloned and characterized this element, which we designate ISRf1. The IS is 1002 bp in length, contains a single 513-bp open reading frame (ORF), is flanked by imperfect 36-bp terminal inverted repeats, and creates 5-bp target duplications. Two copies of ISRf1 are present in the genome of HH103, but it is absent from 12 other Sinorhizobium and Rhizobium strains. The element transposes at a frequency of 2.7 x 10(-6) per generation per cell.</abstract><cop>Netherlands</cop><pmid>9434166</pmid><doi>10.1016/S0378-1119(97)00523-4</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Bacterial Proteins - genetics Base Sequence DNA Transposable Elements - genetics DNA, Bacterial Escherichia coli - metabolism Gene Expression Genes, Bacterial Molecular Sequence Data Rhizobiaceae - genetics |
| title | ISRf1, a transposable insertion sequence from Sinorhizobium fredii |
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