RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors
Nucleocytoplasmic transport appears mediated by shuttling transport receptors that bind RanGTP as a means to regulate interactions with their cargoes. The receptor·RanGTP complexes are kinetically very stable with nucleotide exchange and GTP hydrolysis being blocked, predicting that a specific disas...
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| Veröffentlicht in: | FEBS letters 1997-12, Vol.419 (2), p.249-254 |
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| creator | Bischoff, F.Ralf Görlich, Dirk |
| description | Nucleocytoplasmic transport appears mediated by shuttling transport receptors that bind RanGTP as a means to regulate interactions with their cargoes. The receptor·RanGTP complexes are kinetically very stable with nucleotide exchange and GTP hydrolysis being blocked, predicting that a specific disassembly mechanism exists. Here we show in three cases receptor·RanGTP·RanBP1 complexes to be the key disassembly intermediates, where RanBP1 stimulates the off-rate at the receptor/RanGTP interface by more than two orders of magnitude. The transiently released RanGTP·RanBP1 complex is then induced by RanGAP to hydrolyse GTP, preventing the receptor to rebind RanGTP. The efficient release of importin β from RanGTP requires importin α, in addition to RanBP1. |
| doi_str_mv | 10.1016/S0014-5793(97)01467-1 |
| format | Article |
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The receptor·RanGTP complexes are kinetically very stable with nucleotide exchange and GTP hydrolysis being blocked, predicting that a specific disassembly mechanism exists. Here we show in three cases receptor·RanGTP·RanBP1 complexes to be the key disassembly intermediates, where RanBP1 stimulates the off-rate at the receptor/RanGTP interface by more than two orders of magnitude. The transiently released RanGTP·RanBP1 complex is then induced by RanGAP to hydrolyse GTP, preventing the receptor to rebind RanGTP. The efficient release of importin β from RanGTP requires importin α, in addition to RanBP1.</description><subject>Animals</subject><subject>beta Karyopherins</subject><subject>Biological Transport</subject><subject>CAS</subject><subject>Cell Nucleus - metabolism</subject><subject>GAP</subject><subject>GDP-bound form of Ran</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>GTP-bound form of Ran</subject><subject>GTPase activating protein</subject><subject>Guanosine Triphosphate - metabolism</subject><subject>Humans</subject><subject>Importin</subject><subject>Mice</subject><subject>NPC</subject><subject>nuclear pore complex</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nuclear transport</subject><subject>ran GTP-Binding Protein</subject><subject>RanBP1</subject><subject>RanGDP</subject><subject>RanGTP</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>Recombinant Proteins - metabolism</subject><subject>Transportin</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtq3DAUhkVJSSdpHyGgVUkWTiXZuq1KE3KDQEObbitk6Ziq2NZUslPyWn2QPFPkmSHbdCF0-S9HfAgdUXJKCRWfvhNCm4pLXR9reVLOQlb0DVpRJeuqboTaQ6sXyzt0kPNvUu6K6n20rxumRNOs0M9vdjy7ozhk7NLsgu1xFxOefgFO0IPNgGOHi-nq_g53KQ44DOuYpjDip39VsdgJPB5nV7wlluyYFxl31k0x5ffobWf7DB92-yH6cXlxf35d3X69ujn_clu5Rkpa1eCVd4x7KYTsbMs9sJa4VlPROscZc60AR0WnBOG8LM-U41RIzWrNBNSH6OO2d53inxnyZIaQHfS9HSHO2UjNiVK6Lka-NboUc07QmXUKg02PhhKzcDUbrmaBZrQ0G66GltzRbsDcDuBfUjuQRb_e6n9DD4__V2ouL87YRlkELTfPy6jP2yoowB4CJJNdgNGBDwncZHwMr3z2GWY6nAM</recordid><startdate>19971215</startdate><enddate>19971215</enddate><creator>Bischoff, F.Ralf</creator><creator>Görlich, Dirk</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971215</creationdate><title>RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors</title><author>Bischoff, F.Ralf ; Görlich, Dirk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4771-3ed8dc25d7667fab5de2b0cb916bcc522cb6ec16f86055605d28c5167923926e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>beta Karyopherins</topic><topic>Biological Transport</topic><topic>CAS</topic><topic>Cell Nucleus - metabolism</topic><topic>GAP</topic><topic>GDP-bound form of Ran</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>GTP-bound form of Ran</topic><topic>GTPase activating protein</topic><topic>Guanosine Triphosphate - metabolism</topic><topic>Humans</topic><topic>Importin</topic><topic>Mice</topic><topic>NPC</topic><topic>nuclear pore complex</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nuclear transport</topic><topic>ran GTP-Binding Protein</topic><topic>RanBP1</topic><topic>RanGDP</topic><topic>RanGTP</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>Recombinant Proteins - metabolism</topic><topic>Transportin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bischoff, F.Ralf</creatorcontrib><creatorcontrib>Görlich, Dirk</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bischoff, F.Ralf</au><au>Görlich, Dirk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1997-12-15</date><risdate>1997</risdate><volume>419</volume><issue>2</issue><spage>249</spage><epage>254</epage><pages>249-254</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Nucleocytoplasmic transport appears mediated by shuttling transport receptors that bind RanGTP as a means to regulate interactions with their cargoes. The receptor·RanGTP complexes are kinetically very stable with nucleotide exchange and GTP hydrolysis being blocked, predicting that a specific disassembly mechanism exists. Here we show in three cases receptor·RanGTP·RanBP1 complexes to be the key disassembly intermediates, where RanBP1 stimulates the off-rate at the receptor/RanGTP interface by more than two orders of magnitude. The transiently released RanGTP·RanBP1 complex is then induced by RanGAP to hydrolyse GTP, preventing the receptor to rebind RanGTP. The efficient release of importin β from RanGTP requires importin α, in addition to RanBP1.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9428644</pmid><doi>10.1016/S0014-5793(97)01467-1</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1997-12, Vol.419 (2), p.249-254 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Animals beta Karyopherins Biological Transport CAS Cell Nucleus - metabolism GAP GDP-bound form of Ran GTP-Binding Proteins - metabolism GTP-bound form of Ran GTPase activating protein Guanosine Triphosphate - metabolism Humans Importin Mice NPC nuclear pore complex Nuclear Proteins - metabolism Nuclear transport ran GTP-Binding Protein RanBP1 RanGDP RanGTP Receptors, Cytoplasmic and Nuclear - metabolism Recombinant Proteins - metabolism Transportin |
| title | RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors |
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