Atrial natriuretic peptide stimulates Na+‐dependent Ca2+ efflux from freshly isolated adult rat cardiomyocytes
In the present study, we examined the effect of atrial natriuretic peptide (ANP) on Ca2+ efflux from freshly isolated adult rat cardiomyocytes. Rat ANP(1–28) stimulated the efflux of 45Ca2+ from the cells in a concentration‐dependent manner (10−8 M to 10−6 M). The 45Ca2+ efflux was not affected by r...
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| Veröffentlicht in: | FEBS letters 1997-12, Vol.419 (2-3), p.255-258 |
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| creator | Yoshizumi, Masanori Houchi, Hitoshi Tsuchiya, Koichiro Minakuchi, Kazuo Horike, Kazuya Kitagawa, Tetsuya Katoh, Itsuo Tamaki, Toshiaki |
| description | In the present study, we examined the effect of atrial natriuretic peptide (ANP) on Ca2+ efflux from freshly isolated adult rat cardiomyocytes. Rat ANP(1–28) stimulated the efflux of 45Ca2+ from the cells in a concentration‐dependent manner (10−8 M to 10−6 M). The 45Ca2+ efflux was not affected by removal of extracellular Ca2+, but was dependent on the presence of extracellular Na+. In addition, rat ANP(1–28) caused 22Na+ influx into the cells. The 45Ca2+ efflux was also stimulated by C‐type natriuretic peptide‐22 (CNP‐22), but not by rat brain natriuretic peptide‐45 (BNP‐45). It was also observed that both rat ANP(1–28) and CNP‐22 stimulated guanosine 3′,5′‐cyclic monophosphate production within the cells. These results indicate that ANP stimulates Na+‐dependent 45Ca2+ efflux from freshly isolated adult rat cardiomyocytes, probably through Na+/Ca2+ exchange, and that the stimulatory effect of ANP on Ca2+ efflux may be mediated via the natriuretic peptide receptor which has been shown to couple to guanylate cyclase. Since it is reported that Na+/Ca2+ exchange is important in calcium homeostasis within cells, ANP may play a role in the extrusion of intracellular Ca2+ from isolated adult rat cardiomyocytes. |
| doi_str_mv | 10.1016/S0014-5793(97)01470-1 |
| format | Article |
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Rat ANP(1–28) stimulated the efflux of 45Ca2+ from the cells in a concentration‐dependent manner (10−8 M to 10−6 M). The 45Ca2+ efflux was not affected by removal of extracellular Ca2+, but was dependent on the presence of extracellular Na+. In addition, rat ANP(1–28) caused 22Na+ influx into the cells. The 45Ca2+ efflux was also stimulated by C‐type natriuretic peptide‐22 (CNP‐22), but not by rat brain natriuretic peptide‐45 (BNP‐45). It was also observed that both rat ANP(1–28) and CNP‐22 stimulated guanosine 3′,5′‐cyclic monophosphate production within the cells. These results indicate that ANP stimulates Na+‐dependent 45Ca2+ efflux from freshly isolated adult rat cardiomyocytes, probably through Na+/Ca2+ exchange, and that the stimulatory effect of ANP on Ca2+ efflux may be mediated via the natriuretic peptide receptor which has been shown to couple to guanylate cyclase. Since it is reported that Na+/Ca2+ exchange is important in calcium homeostasis within cells, ANP may play a role in the extrusion of intracellular Ca2+ from isolated adult rat cardiomyocytes.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(97)01470-1</identifier><identifier>PMID: 9428645</identifier><language>eng</language><publisher>England</publisher><subject>[Ca2+]i ; Animals ; ANP ; Atrial Natriuretic Factor - pharmacology ; Atrial natriuretic peptide ; BNP ; brain natriuretic peptide ; C-type natriuretic peptide ; Ca2+ efflux ; Calcium - metabolism ; Cardiomyocyte ; Cells, Cultured ; cGMP ; CNP ; cytosolic free calcium concentration ; Guanosine 3′,5′-cyclic monophosphate ; Ion Transport - drug effects ; K-H solution ; Krebs-Henseleit bicarbonate buffer solution ; Male ; Myocardium - metabolism ; Na+/Ca2+ exchange ; Natriuretic peptide receptor ; NPR ; Rats ; Rats, Sprague-Dawley ; Sodium - metabolism</subject><ispartof>FEBS letters, 1997-12, Vol.419 (2-3), p.255-258</ispartof><rights>FEBS Letters 419 (1997) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2897%2901470-1$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0014-5793%2897%2901470-1$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9428645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshizumi, Masanori</creatorcontrib><creatorcontrib>Houchi, Hitoshi</creatorcontrib><creatorcontrib>Tsuchiya, Koichiro</creatorcontrib><creatorcontrib>Minakuchi, Kazuo</creatorcontrib><creatorcontrib>Horike, Kazuya</creatorcontrib><creatorcontrib>Kitagawa, Tetsuya</creatorcontrib><creatorcontrib>Katoh, Itsuo</creatorcontrib><creatorcontrib>Tamaki, Toshiaki</creatorcontrib><title>Atrial natriuretic peptide stimulates Na+‐dependent Ca2+ efflux from freshly isolated adult rat cardiomyocytes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>In the present study, we examined the effect of atrial natriuretic peptide (ANP) on Ca2+ efflux from freshly isolated adult rat cardiomyocytes. Rat ANP(1–28) stimulated the efflux of 45Ca2+ from the cells in a concentration‐dependent manner (10−8 M to 10−6 M). The 45Ca2+ efflux was not affected by removal of extracellular Ca2+, but was dependent on the presence of extracellular Na+. In addition, rat ANP(1–28) caused 22Na+ influx into the cells. The 45Ca2+ efflux was also stimulated by C‐type natriuretic peptide‐22 (CNP‐22), but not by rat brain natriuretic peptide‐45 (BNP‐45). It was also observed that both rat ANP(1–28) and CNP‐22 stimulated guanosine 3′,5′‐cyclic monophosphate production within the cells. These results indicate that ANP stimulates Na+‐dependent 45Ca2+ efflux from freshly isolated adult rat cardiomyocytes, probably through Na+/Ca2+ exchange, and that the stimulatory effect of ANP on Ca2+ efflux may be mediated via the natriuretic peptide receptor which has been shown to couple to guanylate cyclase. Since it is reported that Na+/Ca2+ exchange is important in calcium homeostasis within cells, ANP may play a role in the extrusion of intracellular Ca2+ from isolated adult rat cardiomyocytes.</description><subject>[Ca2+]i</subject><subject>Animals</subject><subject>ANP</subject><subject>Atrial Natriuretic Factor - pharmacology</subject><subject>Atrial natriuretic peptide</subject><subject>BNP</subject><subject>brain natriuretic peptide</subject><subject>C-type natriuretic peptide</subject><subject>Ca2+ efflux</subject><subject>Calcium - metabolism</subject><subject>Cardiomyocyte</subject><subject>Cells, Cultured</subject><subject>cGMP</subject><subject>CNP</subject><subject>cytosolic free calcium concentration</subject><subject>Guanosine 3′,5′-cyclic monophosphate</subject><subject>Ion Transport - drug effects</subject><subject>K-H solution</subject><subject>Krebs-Henseleit bicarbonate buffer solution</subject><subject>Male</subject><subject>Myocardium - metabolism</subject><subject>Na+/Ca2+ exchange</subject><subject>Natriuretic peptide receptor</subject><subject>NPR</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sodium - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc1O3DAQxy1UtGwpj4DkE2qFUjx2EsfH7QpKpRUcoGfLicfCyNmE2FGbWx-hz9gnafZDXObr9585zJ-QS2BfgUF588QY5Fkhlfis5Je5liyDE7KESopM5GX1gSzfJWfkY4yvbO4rUAuyUDmvyrxYkn6VBm8C3Zo5jwMm39Ae--Qt0ph8OwaTMNIHc_3vz1-LPW4tbhNdG35N0bkw_qZu6No5YHwJE_Wx221YauwYEh1Moo0ZrO_aqWum-dQncupMiHhxzOfk593t8_o-2zx-_7FebbJXXirIoFaiYjVXTkleIxQlQGMra5xoUFiHZV3YHLhDZQRnNRrHgefS1a6WAEKck6vD3X7o3kaMSbc-NhiC2WI3Ri1VwSoAPgsvj8KxbtHqfvCtGSZ9fNHM7w_8lw84vWNgemeD3tugdz_WSuq9DRr03e03vic7oOR-DOI_zSOAeg</recordid><startdate>19971215</startdate><enddate>19971215</enddate><creator>Yoshizumi, Masanori</creator><creator>Houchi, Hitoshi</creator><creator>Tsuchiya, Koichiro</creator><creator>Minakuchi, Kazuo</creator><creator>Horike, Kazuya</creator><creator>Kitagawa, Tetsuya</creator><creator>Katoh, Itsuo</creator><creator>Tamaki, Toshiaki</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19971215</creationdate><title>Atrial natriuretic peptide stimulates Na+‐dependent Ca2+ efflux from freshly isolated adult rat cardiomyocytes</title><author>Yoshizumi, Masanori ; Houchi, Hitoshi ; Tsuchiya, Koichiro ; Minakuchi, Kazuo ; Horike, Kazuya ; Kitagawa, Tetsuya ; Katoh, Itsuo ; Tamaki, Toshiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j2691-1b9380b29f972be15611cd8daf3ce3dfe6b5d412fe9a320beaf21247fbfb71133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>[Ca2+]i</topic><topic>Animals</topic><topic>ANP</topic><topic>Atrial Natriuretic Factor - pharmacology</topic><topic>Atrial natriuretic peptide</topic><topic>BNP</topic><topic>brain natriuretic peptide</topic><topic>C-type natriuretic peptide</topic><topic>Ca2+ efflux</topic><topic>Calcium - metabolism</topic><topic>Cardiomyocyte</topic><topic>Cells, Cultured</topic><topic>cGMP</topic><topic>CNP</topic><topic>cytosolic free calcium concentration</topic><topic>Guanosine 3′,5′-cyclic monophosphate</topic><topic>Ion Transport - drug effects</topic><topic>K-H solution</topic><topic>Krebs-Henseleit bicarbonate buffer solution</topic><topic>Male</topic><topic>Myocardium - metabolism</topic><topic>Na+/Ca2+ exchange</topic><topic>Natriuretic peptide receptor</topic><topic>NPR</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sodium - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshizumi, Masanori</creatorcontrib><creatorcontrib>Houchi, Hitoshi</creatorcontrib><creatorcontrib>Tsuchiya, Koichiro</creatorcontrib><creatorcontrib>Minakuchi, Kazuo</creatorcontrib><creatorcontrib>Horike, Kazuya</creatorcontrib><creatorcontrib>Kitagawa, Tetsuya</creatorcontrib><creatorcontrib>Katoh, Itsuo</creatorcontrib><creatorcontrib>Tamaki, Toshiaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshizumi, Masanori</au><au>Houchi, Hitoshi</au><au>Tsuchiya, Koichiro</au><au>Minakuchi, Kazuo</au><au>Horike, Kazuya</au><au>Kitagawa, Tetsuya</au><au>Katoh, Itsuo</au><au>Tamaki, Toshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atrial natriuretic peptide stimulates Na+‐dependent Ca2+ efflux from freshly isolated adult rat cardiomyocytes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1997-12-15</date><risdate>1997</risdate><volume>419</volume><issue>2-3</issue><spage>255</spage><epage>258</epage><pages>255-258</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>In the present study, we examined the effect of atrial natriuretic peptide (ANP) on Ca2+ efflux from freshly isolated adult rat cardiomyocytes. Rat ANP(1–28) stimulated the efflux of 45Ca2+ from the cells in a concentration‐dependent manner (10−8 M to 10−6 M). The 45Ca2+ efflux was not affected by removal of extracellular Ca2+, but was dependent on the presence of extracellular Na+. In addition, rat ANP(1–28) caused 22Na+ influx into the cells. The 45Ca2+ efflux was also stimulated by C‐type natriuretic peptide‐22 (CNP‐22), but not by rat brain natriuretic peptide‐45 (BNP‐45). It was also observed that both rat ANP(1–28) and CNP‐22 stimulated guanosine 3′,5′‐cyclic monophosphate production within the cells. These results indicate that ANP stimulates Na+‐dependent 45Ca2+ efflux from freshly isolated adult rat cardiomyocytes, probably through Na+/Ca2+ exchange, and that the stimulatory effect of ANP on Ca2+ efflux may be mediated via the natriuretic peptide receptor which has been shown to couple to guanylate cyclase. Since it is reported that Na+/Ca2+ exchange is important in calcium homeostasis within cells, ANP may play a role in the extrusion of intracellular Ca2+ from isolated adult rat cardiomyocytes.</abstract><cop>England</cop><pmid>9428645</pmid><doi>10.1016/S0014-5793(97)01470-1</doi><tpages>4</tpages></addata></record> |
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| subjects | [Ca2+]i Animals ANP Atrial Natriuretic Factor - pharmacology Atrial natriuretic peptide BNP brain natriuretic peptide C-type natriuretic peptide Ca2+ efflux Calcium - metabolism Cardiomyocyte Cells, Cultured cGMP CNP cytosolic free calcium concentration Guanosine 3′,5′-cyclic monophosphate Ion Transport - drug effects K-H solution Krebs-Henseleit bicarbonate buffer solution Male Myocardium - metabolism Na+/Ca2+ exchange Natriuretic peptide receptor NPR Rats Rats, Sprague-Dawley Sodium - metabolism |
| title | Atrial natriuretic peptide stimulates Na+‐dependent Ca2+ efflux from freshly isolated adult rat cardiomyocytes |
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