Antimicrobial resistance testing of Helicobacter pylori: A comparison of etest and disk diffusion methods
Routine antimicrobial resistance testing of Helicobacter pylori is more commonly performed since the correlation between metronidazole resistance and failure to eradicate using this drug, has been made. While resistance testing of H. pylori by Etest is simple to perform, it is expensive compared to...
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| Veröffentlicht in: | Pathology 1997-11, Vol.29 (4), p.411-414 |
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| creator | Midolo, Peter D. Bell, Jan M. Lambert, John R. Turnidge, John D. Grayson, M. Lindsay |
| description | Routine antimicrobial resistance testing of Helicobacter pylori is more commonly performed since the correlation between metronidazole resistance and failure to eradicate using this drug, has been made. While resistance testing of H. pylori by Etest is simple to perform, it is expensive compared to disk diffusion methods. In this study the Etest was compared with a modified Kirby-Bauer disk diffusion (NCCLS) method for routine resistance screening of H. pylori. Fifty one pre-treatment isolates were tested against amoxycillin, metronidazole, tetracycline and erythromycin by both Etest and disk diffusion using NCCLS guideline strength disks. Clarithromycin was tested by Etest only. Nitroimidazole and macrolide resistance were detected using the modified Kirby-Bauer disk diffusion method which correlated with Etest minimum inhibitory concentration (MIC). Resistance rates were 49% for metronidazole and 8% for clanthromycin. Cross resistance occurs with macrolides against H. pylori and allows testing of erythromycin to predict resistance to clarithromycin. The very low MICs obtained with H. pylori against amoxycillin and tetracycline require the use of Etest or lower strength disk methods to be used. |
| doi_str_mv | 10.1080/00313029700169415 |
| format | Article |
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Lindsay</creator><creatorcontrib>Midolo, Peter D. ; Bell, Jan M. ; Lambert, John R. ; Turnidge, John D. ; Grayson, M. Lindsay</creatorcontrib><description>Routine antimicrobial resistance testing of Helicobacter pylori is more commonly performed since the correlation between metronidazole resistance and failure to eradicate using this drug, has been made. While resistance testing of H. pylori by Etest is simple to perform, it is expensive compared to disk diffusion methods. In this study the Etest was compared with a modified Kirby-Bauer disk diffusion (NCCLS) method for routine resistance screening of H. pylori. Fifty one pre-treatment isolates were tested against amoxycillin, metronidazole, tetracycline and erythromycin by both Etest and disk diffusion using NCCLS guideline strength disks. Clarithromycin was tested by Etest only. Nitroimidazole and macrolide resistance were detected using the modified Kirby-Bauer disk diffusion method which correlated with Etest minimum inhibitory concentration (MIC). Resistance rates were 49% for metronidazole and 8% for clanthromycin. Cross resistance occurs with macrolides against H. pylori and allows testing of erythromycin to predict resistance to clarithromycin. The very low MICs obtained with H. pylori against amoxycillin and tetracycline require the use of Etest or lower strength disk methods to be used.</description><identifier>ISSN: 0031-3025</identifier><identifier>EISSN: 1465-3931</identifier><identifier>DOI: 10.1080/00313029700169415</identifier><identifier>PMID: 9423225</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Amoxicillin - pharmacology ; Anti-Bacterial Agents - pharmacology ; antimicrobial resistance ; Biological and medical sciences ; Clarithromycin - pharmacology ; Digestive system ; disk diffusion ; Erythromycin - pharmacology ; Etest ; Female ; Helicobacter pylori ; Helicobacter pylori - drug effects ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Macrolides - pharmacology ; Male ; Medical sciences ; Metronidazole - pharmacology ; Microbial Sensitivity Tests - methods ; Microbiological Techniques ; Middle Aged ; Nitroimidazoles - pharmacology ; Pathology. 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Lindsay</creatorcontrib><title>Antimicrobial resistance testing of Helicobacter pylori: A comparison of etest and disk diffusion methods</title><title>Pathology</title><addtitle>Pathology</addtitle><description>Routine antimicrobial resistance testing of Helicobacter pylori is more commonly performed since the correlation between metronidazole resistance and failure to eradicate using this drug, has been made. While resistance testing of H. pylori by Etest is simple to perform, it is expensive compared to disk diffusion methods. In this study the Etest was compared with a modified Kirby-Bauer disk diffusion (NCCLS) method for routine resistance screening of H. pylori. Fifty one pre-treatment isolates were tested against amoxycillin, metronidazole, tetracycline and erythromycin by both Etest and disk diffusion using NCCLS guideline strength disks. Clarithromycin was tested by Etest only. Nitroimidazole and macrolide resistance were detected using the modified Kirby-Bauer disk diffusion method which correlated with Etest minimum inhibitory concentration (MIC). Resistance rates were 49% for metronidazole and 8% for clanthromycin. Cross resistance occurs with macrolides against H. pylori and allows testing of erythromycin to predict resistance to clarithromycin. The very low MICs obtained with H. pylori against amoxycillin and tetracycline require the use of Etest or lower strength disk methods to be used.</description><subject>Amoxicillin - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antimicrobial resistance</subject><subject>Biological and medical sciences</subject><subject>Clarithromycin - pharmacology</subject><subject>Digestive system</subject><subject>disk diffusion</subject><subject>Erythromycin - pharmacology</subject><subject>Etest</subject><subject>Female</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - drug effects</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Macrolides - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metronidazole - pharmacology</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Microbiological Techniques</subject><subject>Middle Aged</subject><subject>Nitroimidazoles - pharmacology</subject><subject>Pathology. 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Lindsay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-42e5bb14616e9abaeab83bde83921b33db7d6d866b80be4019c62c5b21cfb85f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amoxicillin - pharmacology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antimicrobial resistance</topic><topic>Biological and medical sciences</topic><topic>Clarithromycin - pharmacology</topic><topic>Digestive system</topic><topic>disk diffusion</topic><topic>Erythromycin - pharmacology</topic><topic>Etest</topic><topic>Female</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - drug effects</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Macrolides - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metronidazole - pharmacology</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Microbiological Techniques</topic><topic>Middle Aged</topic><topic>Nitroimidazoles - pharmacology</topic><topic>Pathology. 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Fifty one pre-treatment isolates were tested against amoxycillin, metronidazole, tetracycline and erythromycin by both Etest and disk diffusion using NCCLS guideline strength disks. Clarithromycin was tested by Etest only. Nitroimidazole and macrolide resistance were detected using the modified Kirby-Bauer disk diffusion method which correlated with Etest minimum inhibitory concentration (MIC). Resistance rates were 49% for metronidazole and 8% for clanthromycin. Cross resistance occurs with macrolides against H. pylori and allows testing of erythromycin to predict resistance to clarithromycin. The very low MICs obtained with H. pylori against amoxycillin and tetracycline require the use of Etest or lower strength disk methods to be used.</abstract><cop>London</cop><pub>Elsevier B.V</pub><pmid>9423225</pmid><doi>10.1080/00313029700169415</doi><tpages>4</tpages></addata></record> |
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| ispartof | Pathology, 1997-11, Vol.29 (4), p.411-414 |
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| language | eng |
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| source | MEDLINE; Taylor & Francis Journals Complete; Alma/SFX Local Collection |
| subjects | Amoxicillin - pharmacology Anti-Bacterial Agents - pharmacology antimicrobial resistance Biological and medical sciences Clarithromycin - pharmacology Digestive system disk diffusion Erythromycin - pharmacology Etest Female Helicobacter pylori Helicobacter pylori - drug effects Humans Investigative techniques, diagnostic techniques (general aspects) Macrolides - pharmacology Male Medical sciences Metronidazole - pharmacology Microbial Sensitivity Tests - methods Microbiological Techniques Middle Aged Nitroimidazoles - pharmacology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Tetracycline - pharmacology Tropical medicine |
| title | Antimicrobial resistance testing of Helicobacter pylori: A comparison of etest and disk diffusion methods |
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