Cloning and in situ hybridization analysis of the expression of polysialyltransferase mRNA in the developing and adult rat brain

Polysialyltransferase (PST) is an enzyme that catalyzes the addition of polysialic acid (PSA), a homopolymer of α-2,8-linked sialic acid residues, onto neural cell adhesion molecule (NCAM). The expression of PSA-NCAM in the brain is developmentally regulated and is of critical importance; however, t...

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Veröffentlicht in:Brain research. Molecular brain research. 1997-11, Vol.51 (1), p.69-81
Hauptverfasser: Wood, Graham K, Liang, Jin-Jun, Flores, Gonzalo, Ahmad, Sultan, Quirion, Remi, Srivastava, Lalit K
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container_end_page 81
container_issue 1
container_start_page 69
container_title Brain research. Molecular brain research.
container_volume 51
creator Wood, Graham K
Liang, Jin-Jun
Flores, Gonzalo
Ahmad, Sultan
Quirion, Remi
Srivastava, Lalit K
description Polysialyltransferase (PST) is an enzyme that catalyzes the addition of polysialic acid (PSA), a homopolymer of α-2,8-linked sialic acid residues, onto neural cell adhesion molecule (NCAM). The expression of PSA-NCAM in the brain is developmentally regulated and is of critical importance; however, the temporal and spatial developmental expression of brain PST, a potential key player in the control of PSA-NCAM levels, remains unclear. In the present study, we have cloned the coding region of rat PST cDNA by reverse transcription-polymerase chain reaction, using primers based on the hamster PST-1 cDNA sequence. A 39-mer oligonucleotide complementary to rat PST cDNA was synthesized to investigate the distribution of its mRNA in the developing and adult rat brain by Northern blot and in situ hybridization. In the embryonic rat brain, PST mRNA was detected abundantly throughout the neuroepithelia of most brain regions. At post-natal days 1 and 14, PST was detected throughout the neocortex, in the pyramidal cells (PC) of the hippocampus proper, the granule cell layer (GCL) of the dentate gyrus, the anterior ventral nucleus of the thalamus (AVNT) and the GCL and external germinal layer of the cerebellum. Finally, from PD21 until adulthood, expression of PST mRNA was restricted to the PC layer of the hippocampus proper, the GCL of the dentate gyrus, the AVNT, the GCL of the cerebellum and the dorsal and lateral nucleus of the anterior olfactory bulb. The developmental profile of PST mRNA is paralleled in some structures by that of the PSA-NCAM, there are, however, notable exceptions. Therefore, our results demonstrate that expression of rat PST mRNA is developmentally regulated, is present in the adult rat brain in restricted areas and may be involved in regulating temporal and spatial expression of PSA-NCAM.
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Psychology ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Gestational Age ; Humans ; Hybridization, in situ ; In Situ Hybridization ; Male ; Mice ; Molecular Sequence Data ; mRNA ; Polysialyltransferase ; Pregnancy ; PSA-NCAM ; Rat ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - chemistry ; RNA, Messenger - biosynthesis ; Sequence Alignment ; Sequence Homology, Amino Acid ; Sialyltransferases - biosynthesis ; Sialyltransferases - chemistry ; Transcription, Genetic ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research. 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Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>Polysialyltransferase (PST) is an enzyme that catalyzes the addition of polysialic acid (PSA), a homopolymer of α-2,8-linked sialic acid residues, onto neural cell adhesion molecule (NCAM). The expression of PSA-NCAM in the brain is developmentally regulated and is of critical importance; however, the temporal and spatial developmental expression of brain PST, a potential key player in the control of PSA-NCAM levels, remains unclear. In the present study, we have cloned the coding region of rat PST cDNA by reverse transcription-polymerase chain reaction, using primers based on the hamster PST-1 cDNA sequence. A 39-mer oligonucleotide complementary to rat PST cDNA was synthesized to investigate the distribution of its mRNA in the developing and adult rat brain by Northern blot and in situ hybridization. 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Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wood, Graham K</au><au>Liang, Jin-Jun</au><au>Flores, Gonzalo</au><au>Ahmad, Sultan</au><au>Quirion, Remi</au><au>Srivastava, Lalit K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and in situ hybridization analysis of the expression of polysialyltransferase mRNA in the developing and adult rat brain</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>1997-11-01</date><risdate>1997</risdate><volume>51</volume><issue>1</issue><spage>69</spage><epage>81</epage><pages>69-81</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>Polysialyltransferase (PST) is an enzyme that catalyzes the addition of polysialic acid (PSA), a homopolymer of α-2,8-linked sialic acid residues, onto neural cell adhesion molecule (NCAM). The expression of PSA-NCAM in the brain is developmentally regulated and is of critical importance; however, the temporal and spatial developmental expression of brain PST, a potential key player in the control of PSA-NCAM levels, remains unclear. In the present study, we have cloned the coding region of rat PST cDNA by reverse transcription-polymerase chain reaction, using primers based on the hamster PST-1 cDNA sequence. A 39-mer oligonucleotide complementary to rat PST cDNA was synthesized to investigate the distribution of its mRNA in the developing and adult rat brain by Northern blot and in situ hybridization. In the embryonic rat brain, PST mRNA was detected abundantly throughout the neuroepithelia of most brain regions. At post-natal days 1 and 14, PST was detected throughout the neocortex, in the pyramidal cells (PC) of the hippocampus proper, the granule cell layer (GCL) of the dentate gyrus, the anterior ventral nucleus of the thalamus (AVNT) and the GCL and external germinal layer of the cerebellum. Finally, from PD21 until adulthood, expression of PST mRNA was restricted to the PC layer of the hippocampus proper, the GCL of the dentate gyrus, the AVNT, the GCL of the cerebellum and the dorsal and lateral nucleus of the anterior olfactory bulb. The developmental profile of PST mRNA is paralleled in some structures by that of the PSA-NCAM, there are, however, notable exceptions. Therefore, our results demonstrate that expression of rat PST mRNA is developmentally regulated, is present in the adult rat brain in restricted areas and may be involved in regulating temporal and spatial expression of PSA-NCAM.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9427508</pmid><doi>10.1016/S0169-328X(97)00209-X</doi><tpages>13</tpages></addata></record>
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ispartof Brain research. Molecular brain research., 1997-11, Vol.51 (1), p.69-81
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subjects Aging - metabolism
Amino Acid Sequence
Animals
Biological and medical sciences
Brain
Brain - embryology
Brain - enzymology
Brain - growth & development
Cloning, Molecular - methods
Cricetinae
Development
Development. Senescence. Regeneration. Transplantation
Embryonic and Fetal Development
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Gestational Age
Humans
Hybridization, in situ
In Situ Hybridization
Male
Mice
Molecular Sequence Data
mRNA
Polysialyltransferase
Pregnancy
PSA-NCAM
Rat
Rats
Rats, Sprague-Dawley
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
RNA, Messenger - biosynthesis
Sequence Alignment
Sequence Homology, Amino Acid
Sialyltransferases - biosynthesis
Sialyltransferases - chemistry
Transcription, Genetic
Vertebrates: nervous system and sense organs
title Cloning and in situ hybridization analysis of the expression of polysialyltransferase mRNA in the developing and adult rat brain
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