Kinetic analysis of the Fenton reaction by ESR‐spin trapping
A quantitative analysis of hydroxyl radical (·OH) generated in the Fe2+‐hydrogen peroxide reaction system was explored by a spin‐trapping method using 5,5‐dimethyl‐1‐pyrroline‐N‐oxide (DMPO) combined with electron spin resonance spectroscopy. Based on the numerical analysis of Fenton‐related reactio...
Gespeichert in:
| Veröffentlicht in: | Biochemistry and molecular biology international 1997-12, Vol.43 (5), p.1107-1120 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1120 |
|---|---|
| container_issue | 5 |
| container_start_page | 1107 |
| container_title | Biochemistry and molecular biology international |
| container_volume | 43 |
| creator | Mizuta, Yukio Masumizu, Toshiki Kohno, Masahiro Mori, Akitane Packer, Lester |
| description | A quantitative analysis of hydroxyl radical (·OH) generated in the Fe2+‐hydrogen peroxide reaction system was explored by a spin‐trapping method using 5,5‐dimethyl‐1‐pyrroline‐N‐oxide (DMPO) combined with electron spin resonance spectroscopy. Based on the numerical analysis of Fenton‐related reactions, the reduction of DMPO‐OH adduct from 1…n1 stoichiometry prominent at high concentrations of Fe2+ was consistent with a reaction model in which a molar amount of hydrogen peroxide was reduced by two molar amounts of Fe2+. Furthermore, time‐dependent decrease in DMPO‐OH quantity, apparent at much higher concentration of Fe2+, was proved due to the reaction not with Fe2+ but with Fe3+. |
| doi_str_mv | 10.1080/15216549700204931 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79488445</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79488445</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4150-e23cb0604a7d17695a6f7e732fc5435fb64875a86e69ac2c0c010067809b6f5c3</originalsourceid><addsrcrecordid>eNqFUE1Lw0AQXUSptfoDPAg5eYvOZr-yIIItrYoVQet52Ww3upImMZtScvMn-Bv9JW5p6cWDc3nDezNvhofQKYYLDClcYpZgzqgUAAlQSfAe6q-5mDOG93c9JYfoyPsPCCVA9lBPUszSBPro-sGVtnUm0qUuOu98VOVR-26jiS3bqowaq03rQpN10fjl-efr29eujNpG1wHfjtFBrgtvT7Y4QK-T8Wx0F0-fbu9HN9PYhEMQ24SYDDhQLeZYcMk0z4UVJMlNeI7lGaepYDrllkttEgMGMAAXKciM58yQATrf-NZN9bm0vlUL540tCl3aaumVkDRNKWVhEG8GTVN539hc1Y1b6KZTGNQ6M_Uns7BztjVfZgs7321sQwr61UZfucJ2_xuq2fBxKBgERhAgv4eQdhc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79488445</pqid></control><display><type>article</type><title>Kinetic analysis of the Fenton reaction by ESR‐spin trapping</title><source>Wiley-Blackwell Journals</source><source>MEDLINE</source><source>Wiley Online Library</source><creator>Mizuta, Yukio ; Masumizu, Toshiki ; Kohno, Masahiro ; Mori, Akitane ; Packer, Lester</creator><creatorcontrib>Mizuta, Yukio ; Masumizu, Toshiki ; Kohno, Masahiro ; Mori, Akitane ; Packer, Lester</creatorcontrib><description>A quantitative analysis of hydroxyl radical (·OH) generated in the Fe2+‐hydrogen peroxide reaction system was explored by a spin‐trapping method using 5,5‐dimethyl‐1‐pyrroline‐N‐oxide (DMPO) combined with electron spin resonance spectroscopy. Based on the numerical analysis of Fenton‐related reactions, the reduction of DMPO‐OH adduct from 1…n1 stoichiometry prominent at high concentrations of Fe2+ was consistent with a reaction model in which a molar amount of hydrogen peroxide was reduced by two molar amounts of Fe2+. Furthermore, time‐dependent decrease in DMPO‐OH quantity, apparent at much higher concentration of Fe2+, was proved due to the reaction not with Fe2+ but with Fe3+.</description><identifier>ISSN: 1521-6543</identifier><identifier>ISSN: 1039-9712</identifier><identifier>EISSN: 1521-6551</identifier><identifier>DOI: 10.1080/15216549700204931</identifier><identifier>PMID: 9415820</identifier><language>eng</language><publisher>UK: Informa Healthcare</publisher><subject>Cyclic N-Oxides - metabolism ; Electron Spin Resonance Spectroscopy ; ESR ; Fenton reaction ; Hydrogen Peroxide ; hydroxyl radical ; Hydroxyl Radical - metabolism ; Indicators and Reagents ; Iron ; kinetic analysis ; Kinetics ; Oxidation-Reduction ; Spin Labels ; spin trapping ; Spin Trapping - methods ; stoichiometry</subject><ispartof>Biochemistry and molecular biology international, 1997-12, Vol.43 (5), p.1107-1120</ispartof><rights>Copyright © 1997 International Union of Biochemistry and Molecular Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4150-e23cb0604a7d17695a6f7e732fc5435fb64875a86e69ac2c0c010067809b6f5c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1080%2F15216549700204931$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1080%2F15216549700204931$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9415820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mizuta, Yukio</creatorcontrib><creatorcontrib>Masumizu, Toshiki</creatorcontrib><creatorcontrib>Kohno, Masahiro</creatorcontrib><creatorcontrib>Mori, Akitane</creatorcontrib><creatorcontrib>Packer, Lester</creatorcontrib><title>Kinetic analysis of the Fenton reaction by ESR‐spin trapping</title><title>Biochemistry and molecular biology international</title><addtitle>Biochem Mol Biol Int</addtitle><description>A quantitative analysis of hydroxyl radical (·OH) generated in the Fe2+‐hydrogen peroxide reaction system was explored by a spin‐trapping method using 5,5‐dimethyl‐1‐pyrroline‐N‐oxide (DMPO) combined with electron spin resonance spectroscopy. Based on the numerical analysis of Fenton‐related reactions, the reduction of DMPO‐OH adduct from 1…n1 stoichiometry prominent at high concentrations of Fe2+ was consistent with a reaction model in which a molar amount of hydrogen peroxide was reduced by two molar amounts of Fe2+. Furthermore, time‐dependent decrease in DMPO‐OH quantity, apparent at much higher concentration of Fe2+, was proved due to the reaction not with Fe2+ but with Fe3+.</description><subject>Cyclic N-Oxides - metabolism</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>ESR</subject><subject>Fenton reaction</subject><subject>Hydrogen Peroxide</subject><subject>hydroxyl radical</subject><subject>Hydroxyl Radical - metabolism</subject><subject>Indicators and Reagents</subject><subject>Iron</subject><subject>kinetic analysis</subject><subject>Kinetics</subject><subject>Oxidation-Reduction</subject><subject>Spin Labels</subject><subject>spin trapping</subject><subject>Spin Trapping - methods</subject><subject>stoichiometry</subject><issn>1521-6543</issn><issn>1039-9712</issn><issn>1521-6551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUE1Lw0AQXUSptfoDPAg5eYvOZr-yIIItrYoVQet52Ww3upImMZtScvMn-Bv9JW5p6cWDc3nDezNvhofQKYYLDClcYpZgzqgUAAlQSfAe6q-5mDOG93c9JYfoyPsPCCVA9lBPUszSBPro-sGVtnUm0qUuOu98VOVR-26jiS3bqowaq03rQpN10fjl-efr29eujNpG1wHfjtFBrgtvT7Y4QK-T8Wx0F0-fbu9HN9PYhEMQ24SYDDhQLeZYcMk0z4UVJMlNeI7lGaepYDrllkttEgMGMAAXKciM58yQATrf-NZN9bm0vlUL540tCl3aaumVkDRNKWVhEG8GTVN539hc1Y1b6KZTGNQ6M_Uns7BztjVfZgs7321sQwr61UZfucJ2_xuq2fBxKBgERhAgv4eQdhc</recordid><startdate>199712</startdate><enddate>199712</enddate><creator>Mizuta, Yukio</creator><creator>Masumizu, Toshiki</creator><creator>Kohno, Masahiro</creator><creator>Mori, Akitane</creator><creator>Packer, Lester</creator><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199712</creationdate><title>Kinetic analysis of the Fenton reaction by ESR‐spin trapping</title><author>Mizuta, Yukio ; Masumizu, Toshiki ; Kohno, Masahiro ; Mori, Akitane ; Packer, Lester</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4150-e23cb0604a7d17695a6f7e732fc5435fb64875a86e69ac2c0c010067809b6f5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Cyclic N-Oxides - metabolism</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>ESR</topic><topic>Fenton reaction</topic><topic>Hydrogen Peroxide</topic><topic>hydroxyl radical</topic><topic>Hydroxyl Radical - metabolism</topic><topic>Indicators and Reagents</topic><topic>Iron</topic><topic>kinetic analysis</topic><topic>Kinetics</topic><topic>Oxidation-Reduction</topic><topic>Spin Labels</topic><topic>spin trapping</topic><topic>Spin Trapping - methods</topic><topic>stoichiometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mizuta, Yukio</creatorcontrib><creatorcontrib>Masumizu, Toshiki</creatorcontrib><creatorcontrib>Kohno, Masahiro</creatorcontrib><creatorcontrib>Mori, Akitane</creatorcontrib><creatorcontrib>Packer, Lester</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry and molecular biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mizuta, Yukio</au><au>Masumizu, Toshiki</au><au>Kohno, Masahiro</au><au>Mori, Akitane</au><au>Packer, Lester</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetic analysis of the Fenton reaction by ESR‐spin trapping</atitle><jtitle>Biochemistry and molecular biology international</jtitle><addtitle>Biochem Mol Biol Int</addtitle><date>1997-12</date><risdate>1997</risdate><volume>43</volume><issue>5</issue><spage>1107</spage><epage>1120</epage><pages>1107-1120</pages><issn>1521-6543</issn><issn>1039-9712</issn><eissn>1521-6551</eissn><abstract>A quantitative analysis of hydroxyl radical (·OH) generated in the Fe2+‐hydrogen peroxide reaction system was explored by a spin‐trapping method using 5,5‐dimethyl‐1‐pyrroline‐N‐oxide (DMPO) combined with electron spin resonance spectroscopy. Based on the numerical analysis of Fenton‐related reactions, the reduction of DMPO‐OH adduct from 1…n1 stoichiometry prominent at high concentrations of Fe2+ was consistent with a reaction model in which a molar amount of hydrogen peroxide was reduced by two molar amounts of Fe2+. Furthermore, time‐dependent decrease in DMPO‐OH quantity, apparent at much higher concentration of Fe2+, was proved due to the reaction not with Fe2+ but with Fe3+.</abstract><cop>UK</cop><pub>Informa Healthcare</pub><pmid>9415820</pmid><doi>10.1080/15216549700204931</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1521-6543 |
| ispartof | Biochemistry and molecular biology international, 1997-12, Vol.43 (5), p.1107-1120 |
| issn | 1521-6543 1039-9712 1521-6551 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79488445 |
| source | Wiley-Blackwell Journals; MEDLINE; Wiley Online Library |
| subjects | Cyclic N-Oxides - metabolism Electron Spin Resonance Spectroscopy ESR Fenton reaction Hydrogen Peroxide hydroxyl radical Hydroxyl Radical - metabolism Indicators and Reagents Iron kinetic analysis Kinetics Oxidation-Reduction Spin Labels spin trapping Spin Trapping - methods stoichiometry |
| title | Kinetic analysis of the Fenton reaction by ESR‐spin trapping |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T20%3A14%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Kinetic%20analysis%20of%20the%20Fenton%20reaction%20by%20ESR%E2%80%90spin%20trapping&rft.jtitle=Biochemistry%20and%20molecular%20biology%20international&rft.au=Mizuta,%20Yukio&rft.date=1997-12&rft.volume=43&rft.issue=5&rft.spage=1107&rft.epage=1120&rft.pages=1107-1120&rft.issn=1521-6543&rft.eissn=1521-6551&rft_id=info:doi/10.1080/15216549700204931&rft_dat=%3Cproquest_cross%3E79488445%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79488445&rft_id=info:pmid/9415820&rfr_iscdi=true |