Stimulatory effect of growth hormone on in vitro maturation of bovine oocytes is exerted through the cyclic adenosine 3',5'-monophosphate signaling pathway
The aim of this study was to investigate whether the stimulatory effect of growth hormone (GH) on the in vitro maturation and cumulus expansion of bovine oocytes is exerted through the cAMP or the tyrosine kinase pathway. Therefore bovine cumulus-oocyte complexes (COCs) were cultured in Medium 199 w...
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| Veröffentlicht in: | Biology of reproduction 1997-12, Vol.57 (6), p.1484-1489 |
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| creator | Izadyar, F Colenbrander, B Bevers, M.M |
| description | The aim of this study was to investigate whether the stimulatory effect of growth hormone (GH) on the in vitro maturation
and cumulus expansion of bovine oocytes is exerted through the cAMP or the tyrosine kinase pathway. Therefore bovine cumulus-oocyte
complexes (COCs) were cultured in Medium 199 without fetal calf serum and gonadotropins, but supplemented with 100 ng/ml bovine
GH (bGH; NIH-GH-B18) with or without 10 microM methyl 2,5-dihydroxycinnamate (erbstatin analogue), a specific tyrosine kinase
inhibitor; 100 microM 2',3'-dideoxyadenosine (DDA), a specific adenylate cyclase inhibitor; or 10 microM H-89, a specific
inhibitor of cAMP-dependent protein kinase A. Epidermal growth factor (EGF; 20 ng/ml) was added as a positive control for
tyrosine kinase activation, and FSH (0.05 IU/ml) was added as a positive control for cAMP mediation during in vitro maturation
in the absence or presence of the inhibitors. Culture was performed at 39 degrees C in a humidified atmosphere with 5% CO2
in air. To assess the effect on nuclear maturation, the proportion of oocytes in metaphase II stage after 16 h of culture
was determined using 4,6-diamino-2-phenylindole staining. To determine the effect on cumulus expansion, the diameter of COCs
at the onset and after 24 h of culture was measured. The stimulatory effects of GH on oocyte maturation and cumulus expansion
were blocked by DDA and H-89 (p < 0.01). Similarly, FSH-induced cumulus expansion was abolished by DDA and H-89 (p < 0.05),
while DDA did not block either EGF-induced oocyte maturation or cumulus expansion. Erbstatin analogue significantly blocked
the stimulation of oocyte maturation and cumulus expansion by EGF (p < 0.02) but did not inhibit GH action on the COCs. It
is concluded that the stimulatory effect of GH on oocyte maturation and cumulus expansion is mediated by the cAMP signal transduction
pathway and not by JAK2 phosphorylation. |
| doi_str_mv | 10.1095/biolreprod57.6.1484 |
| format | Article |
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and cumulus expansion of bovine oocytes is exerted through the cAMP or the tyrosine kinase pathway. Therefore bovine cumulus-oocyte
complexes (COCs) were cultured in Medium 199 without fetal calf serum and gonadotropins, but supplemented with 100 ng/ml bovine
GH (bGH; NIH-GH-B18) with or without 10 microM methyl 2,5-dihydroxycinnamate (erbstatin analogue), a specific tyrosine kinase
inhibitor; 100 microM 2',3'-dideoxyadenosine (DDA), a specific adenylate cyclase inhibitor; or 10 microM H-89, a specific
inhibitor of cAMP-dependent protein kinase A. Epidermal growth factor (EGF; 20 ng/ml) was added as a positive control for
tyrosine kinase activation, and FSH (0.05 IU/ml) was added as a positive control for cAMP mediation during in vitro maturation
in the absence or presence of the inhibitors. Culture was performed at 39 degrees C in a humidified atmosphere with 5% CO2
in air. To assess the effect on nuclear maturation, the proportion of oocytes in metaphase II stage after 16 h of culture
was determined using 4,6-diamino-2-phenylindole staining. To determine the effect on cumulus expansion, the diameter of COCs
at the onset and after 24 h of culture was measured. The stimulatory effects of GH on oocyte maturation and cumulus expansion
were blocked by DDA and H-89 (p < 0.01). Similarly, FSH-induced cumulus expansion was abolished by DDA and H-89 (p < 0.05),
while DDA did not block either EGF-induced oocyte maturation or cumulus expansion. Erbstatin analogue significantly blocked
the stimulation of oocyte maturation and cumulus expansion by EGF (p < 0.02) but did not inhibit GH action on the COCs. It
is concluded that the stimulatory effect of GH on oocyte maturation and cumulus expansion is mediated by the cAMP signal transduction
pathway and not by JAK2 phosphorylation.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod57.6.1484</identifier><identifier>PMID: 9408258</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Adenylyl Cyclase Inhibitors ; AMP ; Animals ; Biological and medical sciences ; BOVIN ; C-AMP ; C-AMP SIGNAL TRANSDUCTION ; CATTLE ; Cells, Cultured ; Cinnamates - pharmacology ; Culture Media ; CUMULUS EXPANSION ; CUMULUS OOPHORUS ; Cyclic AMP - physiology ; Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors ; Dideoxyadenosine - pharmacology ; ENZYME INHIBITORS ; Enzyme Inhibitors - pharmacology ; EPIDERMAL GROWTH FACTOR ; Epidermal Growth Factor - pharmacology ; ESTIMULO ; EXPERIMENTACION IN VITRO ; EXPERIMENTATION IN VITRO ; FACTEUR DE CROISSANCE EPIDERMIQUE ; FACTOR DE CRECIMIENTO EPIDERMICO ; Female ; Follicle Stimulating Hormone - pharmacology ; FSH ; Fundamental and applied biological sciences. Psychology ; GANADO BOVINO ; Growth Hormone - pharmacology ; HFS ; Hormone metabolism and regulation ; IN VITRO EXPERIMENTATION ; INHIBIDORES DE ENZIMAS ; INHIBITEUR D'ENZYME ; Isoquinolines - pharmacology ; KINASES ; MADURACION ; Mammalian female genital system ; MATURATION ; Oocytes - physiology ; OVA ; OVULE ; OVULO ; Protein-Tyrosine Kinases - antagonists & inhibitors ; Signal Transduction ; SOMATOTROPIN ; SOMATOTROPINA ; SOMATOTROPINE ; STIMULI ; STIMULUS ; Sulfonamides ; TIROSINA ; TRANSFERASAS ; TRANSFERASE ; TRANSFERASES ; TYROSINE ; TYROSINE KINASE ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1997-12, Vol.57 (6), p.1484-1489</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-5ba4d473c4152072f5b75a9f8db9abcc10f44b4ebc1c1896577d7c788a38d0063</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2092600$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9408258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Izadyar, F</creatorcontrib><creatorcontrib>Colenbrander, B</creatorcontrib><creatorcontrib>Bevers, M.M</creatorcontrib><title>Stimulatory effect of growth hormone on in vitro maturation of bovine oocytes is exerted through the cyclic adenosine 3',5'-monophosphate signaling pathway</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The aim of this study was to investigate whether the stimulatory effect of growth hormone (GH) on the in vitro maturation
and cumulus expansion of bovine oocytes is exerted through the cAMP or the tyrosine kinase pathway. Therefore bovine cumulus-oocyte
complexes (COCs) were cultured in Medium 199 without fetal calf serum and gonadotropins, but supplemented with 100 ng/ml bovine
GH (bGH; NIH-GH-B18) with or without 10 microM methyl 2,5-dihydroxycinnamate (erbstatin analogue), a specific tyrosine kinase
inhibitor; 100 microM 2',3'-dideoxyadenosine (DDA), a specific adenylate cyclase inhibitor; or 10 microM H-89, a specific
inhibitor of cAMP-dependent protein kinase A. Epidermal growth factor (EGF; 20 ng/ml) was added as a positive control for
tyrosine kinase activation, and FSH (0.05 IU/ml) was added as a positive control for cAMP mediation during in vitro maturation
in the absence or presence of the inhibitors. Culture was performed at 39 degrees C in a humidified atmosphere with 5% CO2
in air. To assess the effect on nuclear maturation, the proportion of oocytes in metaphase II stage after 16 h of culture
was determined using 4,6-diamino-2-phenylindole staining. To determine the effect on cumulus expansion, the diameter of COCs
at the onset and after 24 h of culture was measured. The stimulatory effects of GH on oocyte maturation and cumulus expansion
were blocked by DDA and H-89 (p < 0.01). Similarly, FSH-induced cumulus expansion was abolished by DDA and H-89 (p < 0.05),
while DDA did not block either EGF-induced oocyte maturation or cumulus expansion. Erbstatin analogue significantly blocked
the stimulation of oocyte maturation and cumulus expansion by EGF (p < 0.02) but did not inhibit GH action on the COCs. It
is concluded that the stimulatory effect of GH on oocyte maturation and cumulus expansion is mediated by the cAMP signal transduction
pathway and not by JAK2 phosphorylation.</description><subject>Adenylyl Cyclase Inhibitors</subject><subject>AMP</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BOVIN</subject><subject>C-AMP</subject><subject>C-AMP SIGNAL TRANSDUCTION</subject><subject>CATTLE</subject><subject>Cells, Cultured</subject><subject>Cinnamates - pharmacology</subject><subject>Culture Media</subject><subject>CUMULUS EXPANSION</subject><subject>CUMULUS OOPHORUS</subject><subject>Cyclic AMP - physiology</subject><subject>Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors</subject><subject>Dideoxyadenosine - pharmacology</subject><subject>ENZYME INHIBITORS</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>EPIDERMAL GROWTH FACTOR</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>ESTIMULO</subject><subject>EXPERIMENTACION IN VITRO</subject><subject>EXPERIMENTATION IN VITRO</subject><subject>FACTEUR DE CROISSANCE EPIDERMIQUE</subject><subject>FACTOR DE CRECIMIENTO EPIDERMICO</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>FSH</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GANADO BOVINO</subject><subject>Growth Hormone - pharmacology</subject><subject>HFS</subject><subject>Hormone metabolism and regulation</subject><subject>IN VITRO EXPERIMENTATION</subject><subject>INHIBIDORES DE ENZIMAS</subject><subject>INHIBITEUR D'ENZYME</subject><subject>Isoquinolines - pharmacology</subject><subject>KINASES</subject><subject>MADURACION</subject><subject>Mammalian female genital system</subject><subject>MATURATION</subject><subject>Oocytes - physiology</subject><subject>OVA</subject><subject>OVULE</subject><subject>OVULO</subject><subject>Protein-Tyrosine Kinases - antagonists & inhibitors</subject><subject>Signal Transduction</subject><subject>SOMATOTROPIN</subject><subject>SOMATOTROPINA</subject><subject>SOMATOTROPINE</subject><subject>STIMULI</subject><subject>STIMULUS</subject><subject>Sulfonamides</subject><subject>TIROSINA</subject><subject>TRANSFERASAS</subject><subject>TRANSFERASE</subject><subject>TRANSFERASES</subject><subject>TYROSINE</subject><subject>TYROSINE KINASE</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc1u1TAQhSMEKpfCEyAkLxDdkIt_42RZVeVHqsSidG1NHDsxSuKL7TTkWXjZ-qpXhQWrkWa-OWOfUxRvCd4T3IhPrfNjMIfgOyH31Z7wmj8rdkTQppS0qp8XO4xxVTJWsZfFqxh_Ykw4o-ysOGs4rqmod8Wf2-SmZYTkw4aMtUYn5C3qg1_TgAYfJj8b5GfkZnTvUvBogrQESC73Mtj6e3cEvN6SichFZH6bkEyH0hD80g-5GqQ3PTqNoDOzj0eeXXwUF2XW9ofBx8MAyaDo-hlGN_foAGlYYXtdvLAwRvPmVM-Lu8_XP66-ljffv3y7urwptaA0laIF3nHJNM9fx5Ja0UoBja27toFWa4It5y03rSaa1E0lpOyklnUNrO6yQey8-PCom638tZiY1OSiNuMIs_FLVLLhNWGEZZA9gjr4GIOx6hDcBGFTBKtjJOrfSFSljpHkrXcn-aWdTPe0c8ogz9-f5hA1jDbArF18wihuaIXx31cOrh9WF4yKE4xjFmVqXdf_3LPgFfQha93dkqaR2RuBKXsAKCGw3g</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Izadyar, F</creator><creator>Colenbrander, B</creator><creator>Bevers, M.M</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>Stimulatory effect of growth hormone on in vitro maturation of bovine oocytes is exerted through the cyclic adenosine 3',5'-monophosphate signaling pathway</title><author>Izadyar, F ; Colenbrander, B ; Bevers, M.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-5ba4d473c4152072f5b75a9f8db9abcc10f44b4ebc1c1896577d7c788a38d0063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adenylyl Cyclase Inhibitors</topic><topic>AMP</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>BOVIN</topic><topic>C-AMP</topic><topic>C-AMP SIGNAL TRANSDUCTION</topic><topic>CATTLE</topic><topic>Cells, Cultured</topic><topic>Cinnamates - pharmacology</topic><topic>Culture Media</topic><topic>CUMULUS EXPANSION</topic><topic>CUMULUS OOPHORUS</topic><topic>Cyclic AMP - physiology</topic><topic>Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors</topic><topic>Dideoxyadenosine - pharmacology</topic><topic>ENZYME INHIBITORS</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>EPIDERMAL GROWTH FACTOR</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>ESTIMULO</topic><topic>EXPERIMENTACION IN VITRO</topic><topic>EXPERIMENTATION IN VITRO</topic><topic>FACTEUR DE CROISSANCE EPIDERMIQUE</topic><topic>FACTOR DE CRECIMIENTO EPIDERMICO</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>FSH</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GANADO BOVINO</topic><topic>Growth Hormone - pharmacology</topic><topic>HFS</topic><topic>Hormone metabolism and regulation</topic><topic>IN VITRO EXPERIMENTATION</topic><topic>INHIBIDORES DE ENZIMAS</topic><topic>INHIBITEUR D'ENZYME</topic><topic>Isoquinolines - pharmacology</topic><topic>KINASES</topic><topic>MADURACION</topic><topic>Mammalian female genital system</topic><topic>MATURATION</topic><topic>Oocytes - physiology</topic><topic>OVA</topic><topic>OVULE</topic><topic>OVULO</topic><topic>Protein-Tyrosine Kinases - antagonists & inhibitors</topic><topic>Signal Transduction</topic><topic>SOMATOTROPIN</topic><topic>SOMATOTROPINA</topic><topic>SOMATOTROPINE</topic><topic>STIMULI</topic><topic>STIMULUS</topic><topic>Sulfonamides</topic><topic>TIROSINA</topic><topic>TRANSFERASAS</topic><topic>TRANSFERASE</topic><topic>TRANSFERASES</topic><topic>TYROSINE</topic><topic>TYROSINE KINASE</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Izadyar, F</creatorcontrib><creatorcontrib>Colenbrander, B</creatorcontrib><creatorcontrib>Bevers, M.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Izadyar, F</au><au>Colenbrander, B</au><au>Bevers, M.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulatory effect of growth hormone on in vitro maturation of bovine oocytes is exerted through the cyclic adenosine 3',5'-monophosphate signaling pathway</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>57</volume><issue>6</issue><spage>1484</spage><epage>1489</epage><pages>1484-1489</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The aim of this study was to investigate whether the stimulatory effect of growth hormone (GH) on the in vitro maturation
and cumulus expansion of bovine oocytes is exerted through the cAMP or the tyrosine kinase pathway. Therefore bovine cumulus-oocyte
complexes (COCs) were cultured in Medium 199 without fetal calf serum and gonadotropins, but supplemented with 100 ng/ml bovine
GH (bGH; NIH-GH-B18) with or without 10 microM methyl 2,5-dihydroxycinnamate (erbstatin analogue), a specific tyrosine kinase
inhibitor; 100 microM 2',3'-dideoxyadenosine (DDA), a specific adenylate cyclase inhibitor; or 10 microM H-89, a specific
inhibitor of cAMP-dependent protein kinase A. Epidermal growth factor (EGF; 20 ng/ml) was added as a positive control for
tyrosine kinase activation, and FSH (0.05 IU/ml) was added as a positive control for cAMP mediation during in vitro maturation
in the absence or presence of the inhibitors. Culture was performed at 39 degrees C in a humidified atmosphere with 5% CO2
in air. To assess the effect on nuclear maturation, the proportion of oocytes in metaphase II stage after 16 h of culture
was determined using 4,6-diamino-2-phenylindole staining. To determine the effect on cumulus expansion, the diameter of COCs
at the onset and after 24 h of culture was measured. The stimulatory effects of GH on oocyte maturation and cumulus expansion
were blocked by DDA and H-89 (p < 0.01). Similarly, FSH-induced cumulus expansion was abolished by DDA and H-89 (p < 0.05),
while DDA did not block either EGF-induced oocyte maturation or cumulus expansion. Erbstatin analogue significantly blocked
the stimulation of oocyte maturation and cumulus expansion by EGF (p < 0.02) but did not inhibit GH action on the COCs. It
is concluded that the stimulatory effect of GH on oocyte maturation and cumulus expansion is mediated by the cAMP signal transduction
pathway and not by JAK2 phosphorylation.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9408258</pmid><doi>10.1095/biolreprod57.6.1484</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 1997-12, Vol.57 (6), p.1484-1489 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79481313 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adenylyl Cyclase Inhibitors AMP Animals Biological and medical sciences BOVIN C-AMP C-AMP SIGNAL TRANSDUCTION CATTLE Cells, Cultured Cinnamates - pharmacology Culture Media CUMULUS EXPANSION CUMULUS OOPHORUS Cyclic AMP - physiology Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors Dideoxyadenosine - pharmacology ENZYME INHIBITORS Enzyme Inhibitors - pharmacology EPIDERMAL GROWTH FACTOR Epidermal Growth Factor - pharmacology ESTIMULO EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO FACTEUR DE CROISSANCE EPIDERMIQUE FACTOR DE CRECIMIENTO EPIDERMICO Female Follicle Stimulating Hormone - pharmacology FSH Fundamental and applied biological sciences. Psychology GANADO BOVINO Growth Hormone - pharmacology HFS Hormone metabolism and regulation IN VITRO EXPERIMENTATION INHIBIDORES DE ENZIMAS INHIBITEUR D'ENZYME Isoquinolines - pharmacology KINASES MADURACION Mammalian female genital system MATURATION Oocytes - physiology OVA OVULE OVULO Protein-Tyrosine Kinases - antagonists & inhibitors Signal Transduction SOMATOTROPIN SOMATOTROPINA SOMATOTROPINE STIMULI STIMULUS Sulfonamides TIROSINA TRANSFERASAS TRANSFERASE TRANSFERASES TYROSINE TYROSINE KINASE Vertebrates: reproduction |
| title | Stimulatory effect of growth hormone on in vitro maturation of bovine oocytes is exerted through the cyclic adenosine 3',5'-monophosphate signaling pathway |
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