Osteopontin is the 55-kilodalton fertility-associated protein in Holstein bull seminal plasma
Previously we identified four proteins in seminal plasma that were associated with bull fertility. The purpose of this study was to identify the 55-kDa protein prevalent in seminal plasma of higher-fertility males. The 55-kDa protein was quantified by video densitometry in two-dimensional electropho...
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| Veröffentlicht in: | Biology of reproduction 1997-12, Vol.57 (6), p.1293-1301 |
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| creator | Cancel, A.M Chapman, D.A Killian, G.J |
| description | Previously we identified four proteins in seminal plasma that were associated with bull fertility. The purpose of this study
was to identify the 55-kDa protein prevalent in seminal plasma of higher-fertility males. The 55-kDa protein was quantified
by video densitometry in two-dimensional electrophoresis gels of seminal plasma from 26 bulls of known fertility. Relative
density of the 55-kDa protein was positively correlated (r = 0.48) with bull fertility. The 55-kDa (pI 4.5) fertility-associated
protein spot was isolated by electroelution after two-dimensional PAGE separation of seminal plasma of 36 bulls. N-terminal
sequence analysis of the pure protein yielded a 15-amino acid sequence (VKPXSSGXSEEKQLN) that was 86% homologous to bovine
osteopontin-k precursor. Polyclonal antiserum generated against the 55-kDa protein reacted with a single spot in two-dimensional
PAGE Western blots of seminal plasma. Western blot analyses using polyclonal antisera generated against the amino terminus
(LF123) and carboxyl terminus (LF124) of human recombinant osteopontin confirmed that the 55-kDa polypeptide was osteopontin.
Partially purified 55-kDa protein was obtained by HPLC-MonoQ column chromatography. Protein characterization revealed that
the 55-kDa protein was glycosylated, but not phosphorylated, consistent with the identity of the 55-kDa protein as osteopontin. |
| doi_str_mv | 10.1095/biolreprod57.6.1293 |
| format | Article |
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was to identify the 55-kDa protein prevalent in seminal plasma of higher-fertility males. The 55-kDa protein was quantified
by video densitometry in two-dimensional electrophoresis gels of seminal plasma from 26 bulls of known fertility. Relative
density of the 55-kDa protein was positively correlated (r = 0.48) with bull fertility. The 55-kDa (pI 4.5) fertility-associated
protein spot was isolated by electroelution after two-dimensional PAGE separation of seminal plasma of 36 bulls. N-terminal
sequence analysis of the pure protein yielded a 15-amino acid sequence (VKPXSSGXSEEKQLN) that was 86% homologous to bovine
osteopontin-k precursor. Polyclonal antiserum generated against the 55-kDa protein reacted with a single spot in two-dimensional
PAGE Western blots of seminal plasma. Western blot analyses using polyclonal antisera generated against the amino terminus
(LF123) and carboxyl terminus (LF124) of human recombinant osteopontin confirmed that the 55-kDa polypeptide was osteopontin.
Partially purified 55-kDa protein was obtained by HPLC-MonoQ column chromatography. Protein characterization revealed that
the 55-kDa protein was glycosylated, but not phosphorylated, consistent with the identity of the 55-kDa protein as osteopontin.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod57.6.1293</identifier><identifier>PMID: 9408233</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Amino Acid Sequence ; AMINO ACID SEQUENCES ; Animals ; Biological and medical sciences ; Blotting, Western ; BREEDS (ANIMALS) ; BULLS ; Cattle ; CHEMICAL COMPOSITION ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; Densitometry - methods ; Electrophoresis, Gel, Two-Dimensional ; FERTILIDAD ; FERTILITE ; FERTILITY ; Fundamental and applied biological sciences. Psychology ; GLICOPROTEINAS ; GLYCOPROTEINE ; GLYCOPROTEINS ; Glycosylation ; HOLSTEIN-FRIESIAN ; MACHO ; MALE ; MALES ; Mammalian male genital system ; Molecular Sequence Data ; Morphology. Physiology ; Osteopontin ; Phosphorylation ; PURIFICACION ; PURIFICATION ; RACE (ANIMAL) ; RAZAS (ANIMALES) ; SEMEN ; Semen - chemistry ; Sequence Analysis ; Sialoglycoproteins - analysis ; Sialoglycoproteins - chemistry ; SPERME ; TAUREAU ; TORO ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1997-12, Vol.57 (6), p.1293-1301</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2090308$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9408233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cancel, A.M</creatorcontrib><creatorcontrib>Chapman, D.A</creatorcontrib><creatorcontrib>Killian, G.J</creatorcontrib><title>Osteopontin is the 55-kilodalton fertility-associated protein in Holstein bull seminal plasma</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Previously we identified four proteins in seminal plasma that were associated with bull fertility. The purpose of this study
was to identify the 55-kDa protein prevalent in seminal plasma of higher-fertility males. The 55-kDa protein was quantified
by video densitometry in two-dimensional electrophoresis gels of seminal plasma from 26 bulls of known fertility. Relative
density of the 55-kDa protein was positively correlated (r = 0.48) with bull fertility. The 55-kDa (pI 4.5) fertility-associated
protein spot was isolated by electroelution after two-dimensional PAGE separation of seminal plasma of 36 bulls. N-terminal
sequence analysis of the pure protein yielded a 15-amino acid sequence (VKPXSSGXSEEKQLN) that was 86% homologous to bovine
osteopontin-k precursor. Polyclonal antiserum generated against the 55-kDa protein reacted with a single spot in two-dimensional
PAGE Western blots of seminal plasma. Western blot analyses using polyclonal antisera generated against the amino terminus
(LF123) and carboxyl terminus (LF124) of human recombinant osteopontin confirmed that the 55-kDa polypeptide was osteopontin.
Partially purified 55-kDa protein was obtained by HPLC-MonoQ column chromatography. Protein characterization revealed that
the 55-kDa protein was glycosylated, but not phosphorylated, consistent with the identity of the 55-kDa protein as osteopontin.</description><subject>Amino Acid Sequence</subject><subject>AMINO ACID SEQUENCES</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>BREEDS (ANIMALS)</subject><subject>BULLS</subject><subject>Cattle</subject><subject>CHEMICAL COMPOSITION</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>Densitometry - methods</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>FERTILIDAD</subject><subject>FERTILITE</subject><subject>FERTILITY</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>GLYCOPROTEINS</subject><subject>Glycosylation</subject><subject>HOLSTEIN-FRIESIAN</subject><subject>MACHO</subject><subject>MALE</subject><subject>MALES</subject><subject>Mammalian male genital system</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Osteopontin</subject><subject>Phosphorylation</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>RACE (ANIMAL)</subject><subject>RAZAS (ANIMALES)</subject><subject>SEMEN</subject><subject>Semen - chemistry</subject><subject>Sequence Analysis</subject><subject>Sialoglycoproteins - analysis</subject><subject>Sialoglycoproteins - chemistry</subject><subject>SPERME</subject><subject>TAUREAU</subject><subject>TORO</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcFu1DAQtVBRuy18QVUph4pblrHHduIjqlpaqVIP0COyJonTdXHiJfZq1b_HhRVw4zQzem_eG71h7JzDmoNRHzsfw-K2SxxUs9ZrLgy-YSuuhKkbodsjtgIAXSNqPGGnKT0DcIkCj9mxkdAKxBX79pCyi9s4Zz9XPlV54yql6u8-xIFCjnM1uiX74PNLTSnF3lN2Q1VMs3vdmKvbGNKvvtuFUCU3-ZlCtQ2UJnrH3o4Uknt_qGfs8eb669Vtff_w-e7q0309Ije55lI0facVJ0IwozIDoeg4aNXqBvVIsqUeNMDQuW7oO6GpLbgskzI49njGPvzWLXf92LmU7eRT70Kg2cVdso2RLRe8_S-RaxRCoizEiwNx101usNvFT7S82ENwBb884JR6CuNCc-_TH5oAAwj_-G3802bvF2dLKiEUUbT7_V41VtvXv_31GylaelqK1uMXbkwDjZBG4U_0gJW7</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Cancel, A.M</creator><creator>Chapman, D.A</creator><creator>Killian, G.J</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>Osteopontin is the 55-kilodalton fertility-associated protein in Holstein bull seminal plasma</title><author>Cancel, A.M ; Chapman, D.A ; Killian, G.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f319t-1427cb651aa309f59da32b106586736fa48ac0600dbebdcb26a82b14beb593fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>AMINO ACID SEQUENCES</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>BREEDS (ANIMALS)</topic><topic>BULLS</topic><topic>Cattle</topic><topic>CHEMICAL COMPOSITION</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>Densitometry - methods</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>FERTILIDAD</topic><topic>FERTILITE</topic><topic>FERTILITY</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>GLYCOPROTEINS</topic><topic>Glycosylation</topic><topic>HOLSTEIN-FRIESIAN</topic><topic>MACHO</topic><topic>MALE</topic><topic>MALES</topic><topic>Mammalian male genital system</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Osteopontin</topic><topic>Phosphorylation</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>RACE (ANIMAL)</topic><topic>RAZAS (ANIMALES)</topic><topic>SEMEN</topic><topic>Semen - chemistry</topic><topic>Sequence Analysis</topic><topic>Sialoglycoproteins - analysis</topic><topic>Sialoglycoproteins - chemistry</topic><topic>SPERME</topic><topic>TAUREAU</topic><topic>TORO</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cancel, A.M</creatorcontrib><creatorcontrib>Chapman, D.A</creatorcontrib><creatorcontrib>Killian, G.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cancel, A.M</au><au>Chapman, D.A</au><au>Killian, G.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteopontin is the 55-kilodalton fertility-associated protein in Holstein bull seminal plasma</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>57</volume><issue>6</issue><spage>1293</spage><epage>1301</epage><pages>1293-1301</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Previously we identified four proteins in seminal plasma that were associated with bull fertility. The purpose of this study
was to identify the 55-kDa protein prevalent in seminal plasma of higher-fertility males. The 55-kDa protein was quantified
by video densitometry in two-dimensional electrophoresis gels of seminal plasma from 26 bulls of known fertility. Relative
density of the 55-kDa protein was positively correlated (r = 0.48) with bull fertility. The 55-kDa (pI 4.5) fertility-associated
protein spot was isolated by electroelution after two-dimensional PAGE separation of seminal plasma of 36 bulls. N-terminal
sequence analysis of the pure protein yielded a 15-amino acid sequence (VKPXSSGXSEEKQLN) that was 86% homologous to bovine
osteopontin-k precursor. Polyclonal antiserum generated against the 55-kDa protein reacted with a single spot in two-dimensional
PAGE Western blots of seminal plasma. Western blot analyses using polyclonal antisera generated against the amino terminus
(LF123) and carboxyl terminus (LF124) of human recombinant osteopontin confirmed that the 55-kDa polypeptide was osteopontin.
Partially purified 55-kDa protein was obtained by HPLC-MonoQ column chromatography. Protein characterization revealed that
the 55-kDa protein was glycosylated, but not phosphorylated, consistent with the identity of the 55-kDa protein as osteopontin.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9408233</pmid><doi>10.1095/biolreprod57.6.1293</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1997-12, Vol.57 (6), p.1293-1301 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79481218 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Amino Acid Sequence AMINO ACID SEQUENCES Animals Biological and medical sciences Blotting, Western BREEDS (ANIMALS) BULLS Cattle CHEMICAL COMPOSITION COMPOSICION QUIMICA COMPOSITION CHIMIQUE Densitometry - methods Electrophoresis, Gel, Two-Dimensional FERTILIDAD FERTILITE FERTILITY Fundamental and applied biological sciences. Psychology GLICOPROTEINAS GLYCOPROTEINE GLYCOPROTEINS Glycosylation HOLSTEIN-FRIESIAN MACHO MALE MALES Mammalian male genital system Molecular Sequence Data Morphology. Physiology Osteopontin Phosphorylation PURIFICACION PURIFICATION RACE (ANIMAL) RAZAS (ANIMALES) SEMEN Semen - chemistry Sequence Analysis Sialoglycoproteins - analysis Sialoglycoproteins - chemistry SPERME TAUREAU TORO Vertebrates: reproduction |
| title | Osteopontin is the 55-kilodalton fertility-associated protein in Holstein bull seminal plasma |
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