Selection of optimum affinity tags from a phage-displayed peptide library Application to immobilized copper(II) affinity chromatography

Immobilized metal affinity chromatography (IMAC) is a versatile tool for the purification of proteins with affinity for immobilized metals. Moreover, this technique has also been used for the separation of proteins that do not exhibit significant metal affinity in the native form, by their fusion to...

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Veröffentlicht in:	Journal of Chromatography A 1997-11, Vol.787 (1), p.91-100
Hauptverfasser:	Patwardhan, A.V., Goud, G.N., Koepsel, R.R., Ataai, M.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Affinity tags Analytical, structural and metabolic biochemistry Biological and medical sciences Chelating Agents Chromatography, Affinity - methods Coliphages - genetics Copper - chemistry Fundamental and applied biological sciences. Psychology General aspects, investigation methods Histidine - chemistry Humans Hydrogen-Ion Concentration Imino Acids Immobilized metal affinity chromatography Interleukin Interleukin-1 - genetics Interleukin-1 - isolation & purification Peptide Library Peptides Peptides - genetics Phage display library Proteins Proteins - genetics Proteins - isolation & purification Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics
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