Leucine sources for 10.5-day rat conceptus in vivo
Protein has been shown to be the principal source of leucine for the Day 8.5 to 10.5 rat conceptus in culture. It could be argued that this finding applies only after adaptation to culture conditions and does not apply in vivo. This possibility was investigated using an isotope-dilution technique af...
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| Veröffentlicht in: | Reproductive toxicology (Elmsford, N.Y.) N.Y.), 1997-11, Vol.11 (6), p.875-877 |
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| container_title | Reproductive toxicology (Elmsford, N.Y.) |
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| creator | Beckman, David A. Tu, Christina |
| description | Protein has been shown to be the principal source of leucine for the Day 8.5 to 10.5 rat conceptus in culture. It could be argued that this finding applies only after adaptation to culture conditions and does not apply in vivo. This possibility was investigated using an isotope-dilution technique after I.V. injection of [
3H]leucine into Day 10.5 pregnant rats. Specific radioactivity of free leucine in the conceptus was 8 to 10% of that in maternal plasma. Slow exchange of leucine with the maternal circulation and fetal tissue protein turnover were judged to be inadequate as explanations for the observed isotope dilution. Taken together, our results and those from in vitro studies are consistent with a major contribution of leucine coming from the degradation of protein in vivo, probably involving the visceral yolk sac. Our results suggest that mechanisms of amino acid supply to the conceptus identified using whole-embryo culture mirror those in vivo. |
| doi_str_mv | 10.1016/S0890-6238(97)00071-3 |
| format | Article |
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3H]leucine into Day 10.5 pregnant rats. Specific radioactivity of free leucine in the conceptus was 8 to 10% of that in maternal plasma. Slow exchange of leucine with the maternal circulation and fetal tissue protein turnover were judged to be inadequate as explanations for the observed isotope dilution. Taken together, our results and those from in vitro studies are consistent with a major contribution of leucine coming from the degradation of protein in vivo, probably involving the visceral yolk sac. Our results suggest that mechanisms of amino acid supply to the conceptus identified using whole-embryo culture mirror those in vivo.</description><identifier>ISSN: 0890-6238</identifier><identifier>EISSN: 1873-1708</identifier><identifier>DOI: 10.1016/S0890-6238(97)00071-3</identifier><identifier>PMID: 9407598</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>amino acid ; Animals ; Biological and medical sciences ; embryo ; Embryo, Mammalian - metabolism ; Embryology: invertebrates and vertebrates. Teratology ; Female ; fetus ; Fundamental and applied biological sciences. Psychology ; leucine ; Leucine - metabolism ; Organogenesis. Physiological fonctions ; Physiological fonctions ; Pregnancy - metabolism ; Rats ; Rats, Wistar ; visceral yolk sac</subject><ispartof>Reproductive toxicology (Elmsford, N.Y.), 1997-11, Vol.11 (6), p.875-877</ispartof><rights>1997</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-38dca7ce44324374ac82265231edee525ff4d6fe855239459b50020c74c6f3643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0890-6238(97)00071-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2118323$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9407598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beckman, David A.</creatorcontrib><creatorcontrib>Tu, Christina</creatorcontrib><title>Leucine sources for 10.5-day rat conceptus in vivo</title><title>Reproductive toxicology (Elmsford, N.Y.)</title><addtitle>Reprod Toxicol</addtitle><description>Protein has been shown to be the principal source of leucine for the Day 8.5 to 10.5 rat conceptus in culture. It could be argued that this finding applies only after adaptation to culture conditions and does not apply in vivo. This possibility was investigated using an isotope-dilution technique after I.V. injection of [
3H]leucine into Day 10.5 pregnant rats. Specific radioactivity of free leucine in the conceptus was 8 to 10% of that in maternal plasma. Slow exchange of leucine with the maternal circulation and fetal tissue protein turnover were judged to be inadequate as explanations for the observed isotope dilution. Taken together, our results and those from in vitro studies are consistent with a major contribution of leucine coming from the degradation of protein in vivo, probably involving the visceral yolk sac. Our results suggest that mechanisms of amino acid supply to the conceptus identified using whole-embryo culture mirror those in vivo.</description><subject>amino acid</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>embryo</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>leucine</subject><subject>Leucine - metabolism</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Physiological fonctions</subject><subject>Pregnancy - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>visceral yolk sac</subject><issn>0890-6238</issn><issn>1873-1708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotVZ_QmEPInpYzecmOYkUv6DgQT2HNDsLkXZTk91C_71pu_TqaWDmeWeGB6EpwfcEk-rhEyuNy4oydavlHcZYkpKdoDFRkpVEYnWKxkfkHF2k9JMhLrUcoZHmWAqtxojOoXe-hSKFPjpIRRNikU-IsrbbItqucKF1sO76VPi22PhNuERnjV0muBrqBH2_PH_N3sr5x-v77GleOk5xVzJVOysdcM4oZ5JbpyitBGUEagBBRdPwumpAidzTXOiFwJhiJ7mrGlZxNkE3h73rGH57SJ1Z-eRgubQthD4ZqblUhJF_QVIxLKjWGRQH0MWQUoTGrKNf2bg1BJudVLOXanbGjJZmL9WwnJsOB_rFCupjarCY59fD3CZnl020rfPpiFFCFKO7NY8HDLK1jYdokvOQ7dY-gutMHfw_j_wBKzuP_A</recordid><startdate>19971101</startdate><enddate>19971101</enddate><creator>Beckman, David A.</creator><creator>Tu, Christina</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19971101</creationdate><title>Leucine sources for 10.5-day rat conceptus in vivo</title><author>Beckman, David A. ; Tu, Christina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-38dca7ce44324374ac82265231edee525ff4d6fe855239459b50020c74c6f3643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>amino acid</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>embryo</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>leucine</topic><topic>Leucine - metabolism</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Physiological fonctions</topic><topic>Pregnancy - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>visceral yolk sac</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beckman, David A.</creatorcontrib><creatorcontrib>Tu, Christina</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive toxicology (Elmsford, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beckman, David A.</au><au>Tu, Christina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leucine sources for 10.5-day rat conceptus in vivo</atitle><jtitle>Reproductive toxicology (Elmsford, N.Y.)</jtitle><addtitle>Reprod Toxicol</addtitle><date>1997-11-01</date><risdate>1997</risdate><volume>11</volume><issue>6</issue><spage>875</spage><epage>877</epage><pages>875-877</pages><issn>0890-6238</issn><eissn>1873-1708</eissn><abstract>Protein has been shown to be the principal source of leucine for the Day 8.5 to 10.5 rat conceptus in culture. It could be argued that this finding applies only after adaptation to culture conditions and does not apply in vivo. This possibility was investigated using an isotope-dilution technique after I.V. injection of [
3H]leucine into Day 10.5 pregnant rats. Specific radioactivity of free leucine in the conceptus was 8 to 10% of that in maternal plasma. Slow exchange of leucine with the maternal circulation and fetal tissue protein turnover were judged to be inadequate as explanations for the observed isotope dilution. Taken together, our results and those from in vitro studies are consistent with a major contribution of leucine coming from the degradation of protein in vivo, probably involving the visceral yolk sac. Our results suggest that mechanisms of amino acid supply to the conceptus identified using whole-embryo culture mirror those in vivo.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9407598</pmid><doi>10.1016/S0890-6238(97)00071-3</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0890-6238 |
| ispartof | Reproductive toxicology (Elmsford, N.Y.), 1997-11, Vol.11 (6), p.875-877 |
| issn | 0890-6238 1873-1708 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | amino acid Animals Biological and medical sciences embryo Embryo, Mammalian - metabolism Embryology: invertebrates and vertebrates. Teratology Female fetus Fundamental and applied biological sciences. Psychology leucine Leucine - metabolism Organogenesis. Physiological fonctions Physiological fonctions Pregnancy - metabolism Rats Rats, Wistar visceral yolk sac |
| title | Leucine sources for 10.5-day rat conceptus in vivo |
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