Seizure-like activity and cellular damage in rat hippocampal neurons in cell culture
Neurons dissociated from the hippocampal formations of neonatal rats were grown in medium containing kynurenic acid (a glutamate receptor antagonist) and elevated Mg 2+. Such chronically blocked neurons, when first exposed to medium without blockers (after 0.5 – 5.0 months), generated intense seizur...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1989-08, Vol.3 (2), p.199-207 |
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| creator | Furshpan, E.J. Potter, D.D. |
| description | Neurons dissociated from the hippocampal formations of neonatal rats were grown in medium containing kynurenic acid (a glutamate receptor antagonist) and elevated Mg
2+. Such chronically blocked neurons, when first exposed to medium without blockers (after 0.5 – 5.0 months), generated intense seizure-like activity. This consisted of bursts of synchronous electrical responses that resembled paroxysmal depolarization shifts and sustained depolarizations that, in some neurons, nearly abolished the resting potential. Sustained depolarizations were usually reversed by timely application of kynurenate or 2-amino-5-phosphonovalerate, indicating that continuous activation of glutamate receptors was required for their maintenance. Prolonged periods of intense seizure-like activity usually killed most neurons in the culture. This system allows seizure-related cellular mechanisms to be studied in long-term cell culture. |
| doi_str_mv | 10.1016/0896-6273(89)90033-0 |
| format | Article |
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2+. Such chronically blocked neurons, when first exposed to medium without blockers (after 0.5 – 5.0 months), generated intense seizure-like activity. This consisted of bursts of synchronous electrical responses that resembled paroxysmal depolarization shifts and sustained depolarizations that, in some neurons, nearly abolished the resting potential. Sustained depolarizations were usually reversed by timely application of kynurenate or 2-amino-5-phosphonovalerate, indicating that continuous activation of glutamate receptors was required for their maintenance. Prolonged periods of intense seizure-like activity usually killed most neurons in the culture. This system allows seizure-related cellular mechanisms to be studied in long-term cell culture.</description><identifier>ISSN: 0896-6273</identifier><identifier>EISSN: 1097-4199</identifier><identifier>DOI: 10.1016/0896-6273(89)90033-0</identifier><identifier>PMID: 2560392</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Survival - physiology ; Cells, Cultured ; Electric Conductivity - drug effects ; Electric Conductivity - physiology ; Hippocampus - cytology ; Hippocampus - physiopathology ; Hippocampus - ultrastructure ; Kynurenic Acid - pharmacology ; Magnesium - pharmacology ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Neurons - cytology ; Neurons - physiology ; Neurons - ultrastructure ; Rats ; Receptors, Glutamate ; Receptors, Neurotransmitter - drug effects ; Seizures - physiopathology</subject><ispartof>Neuron (Cambridge, Mass.), 1989-08, Vol.3 (2), p.199-207</ispartof><rights>1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-2ea6f18ec0e13d8b95b5ef032c37cd17dc4431004d9d14eb8077776ffc0bfb763</citedby><cites>FETCH-LOGICAL-c423t-2ea6f18ec0e13d8b95b5ef032c37cd17dc4431004d9d14eb8077776ffc0bfb763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0896627389900330$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2560392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Furshpan, E.J.</creatorcontrib><creatorcontrib>Potter, D.D.</creatorcontrib><title>Seizure-like activity and cellular damage in rat hippocampal neurons in cell culture</title><title>Neuron (Cambridge, Mass.)</title><addtitle>Neuron</addtitle><description>Neurons dissociated from the hippocampal formations of neonatal rats were grown in medium containing kynurenic acid (a glutamate receptor antagonist) and elevated Mg
2+. Such chronically blocked neurons, when first exposed to medium without blockers (after 0.5 – 5.0 months), generated intense seizure-like activity. This consisted of bursts of synchronous electrical responses that resembled paroxysmal depolarization shifts and sustained depolarizations that, in some neurons, nearly abolished the resting potential. Sustained depolarizations were usually reversed by timely application of kynurenate or 2-amino-5-phosphonovalerate, indicating that continuous activation of glutamate receptors was required for their maintenance. Prolonged periods of intense seizure-like activity usually killed most neurons in the culture. This system allows seizure-related cellular mechanisms to be studied in long-term cell culture.</description><subject>Animals</subject><subject>Cell Survival - physiology</subject><subject>Cells, Cultured</subject><subject>Electric Conductivity - drug effects</subject><subject>Electric Conductivity - physiology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - physiopathology</subject><subject>Hippocampus - ultrastructure</subject><subject>Kynurenic Acid - pharmacology</subject><subject>Magnesium - pharmacology</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>Neurons - ultrastructure</subject><subject>Rats</subject><subject>Receptors, Glutamate</subject><subject>Receptors, Neurotransmitter - drug effects</subject><subject>Seizures - physiopathology</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMoun78A4WcRA_VSZNtmosgi18geHA9hzSZarRfJu2C_npbd_HoXObwvvPOzEPIMYMLBiy7hFxlSZZKfparcwXAeQJbZMZAyUQwpbbJ7M-yR_ZjfAdgYq7YLtlN5xlwlc7I8hn99xAwqfwHUmN7v_L9FzWNoxaraqhMoM7U5hWpb2gwPX3zXddaU3emog0OoW3iJE1uaoeqH8MOyU5pqohHm35AXm5vlov75PHp7mFx_ZhYkfI-SdFkJcvRAjLu8kLNizmWwFPLpXVMOisEZwDCKccEFjnIsbKytFCUhcz4ATld53ah_Rww9rr2cTrENNgOUUslpFQyHY1ibbShjTFgqbvgaxO-NAM9wdQTKT2R0rnSvzA1jGMnm_yhqNH9DW3ojfrVWsfxyZXHoKP12Fh0PqDttWv9_wt-AFQThEY</recordid><startdate>19890801</startdate><enddate>19890801</enddate><creator>Furshpan, E.J.</creator><creator>Potter, D.D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890801</creationdate><title>Seizure-like activity and cellular damage in rat hippocampal neurons in cell culture</title><author>Furshpan, E.J. ; Potter, D.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-2ea6f18ec0e13d8b95b5ef032c37cd17dc4431004d9d14eb8077776ffc0bfb763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Cell Survival - physiology</topic><topic>Cells, Cultured</topic><topic>Electric Conductivity - drug effects</topic><topic>Electric Conductivity - physiology</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - physiopathology</topic><topic>Hippocampus - ultrastructure</topic><topic>Kynurenic Acid - pharmacology</topic><topic>Magnesium - pharmacology</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>Neurons - ultrastructure</topic><topic>Rats</topic><topic>Receptors, Glutamate</topic><topic>Receptors, Neurotransmitter - drug effects</topic><topic>Seizures - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furshpan, E.J.</creatorcontrib><creatorcontrib>Potter, D.D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furshpan, E.J.</au><au>Potter, D.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seizure-like activity and cellular damage in rat hippocampal neurons in cell culture</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>1989-08-01</date><risdate>1989</risdate><volume>3</volume><issue>2</issue><spage>199</spage><epage>207</epage><pages>199-207</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><abstract>Neurons dissociated from the hippocampal formations of neonatal rats were grown in medium containing kynurenic acid (a glutamate receptor antagonist) and elevated Mg
2+. Such chronically blocked neurons, when first exposed to medium without blockers (after 0.5 – 5.0 months), generated intense seizure-like activity. This consisted of bursts of synchronous electrical responses that resembled paroxysmal depolarization shifts and sustained depolarizations that, in some neurons, nearly abolished the resting potential. Sustained depolarizations were usually reversed by timely application of kynurenate or 2-amino-5-phosphonovalerate, indicating that continuous activation of glutamate receptors was required for their maintenance. Prolonged periods of intense seizure-like activity usually killed most neurons in the culture. This system allows seizure-related cellular mechanisms to be studied in long-term cell culture.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2560392</pmid><doi>10.1016/0896-6273(89)90033-0</doi><tpages>9</tpages></addata></record> |
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| ispartof | Neuron (Cambridge, Mass.), 1989-08, Vol.3 (2), p.199-207 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Cell Survival - physiology Cells, Cultured Electric Conductivity - drug effects Electric Conductivity - physiology Hippocampus - cytology Hippocampus - physiopathology Hippocampus - ultrastructure Kynurenic Acid - pharmacology Magnesium - pharmacology Membrane Potentials - drug effects Membrane Potentials - physiology Neurons - cytology Neurons - physiology Neurons - ultrastructure Rats Receptors, Glutamate Receptors, Neurotransmitter - drug effects Seizures - physiopathology |
| title | Seizure-like activity and cellular damage in rat hippocampal neurons in cell culture |
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