Organophosphorus Hydrolase Is a Remarkably Stable Enzyme That Unfolds through a Homodimeric Intermediate

Organophosphorus hydrolase (OPH, EC 8.1.3.1) is a homodimeric enzyme that catalyzes the hydrolysis of organophosphorus pesticides and nerve agents. We have analyzed the urea- and guanidinium chloride-induced equilibrium unfolding of OPH as monitored by far-ultraviolet circular dichroism and intrinsi...

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Veröffentlicht in:	Biochemistry (Easton) 1997-11, Vol.36 (47), p.14366-14374
Hauptverfasser:	Grimsley, Janet K, Scholtz, J. Martin, Pace, C. Nick, Wild, James R
Format:	Artikel
Sprache:	eng
Schlagworte:	agrochemicals Aryldialkylphosphatase Calorimetry Chromatography, Gel Computer Graphics Computer Simulation Dimerization Escherichia coli Esterases - chemistry Esterases - metabolism Guanidine Kinetics Models, Chemical Models, Molecular pesticides Protein Denaturation Protein Folding Protein Structure, Secondary Recombinant Proteins - chemistry Recombinant Proteins - metabolism Thermodynamics Urea
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