An indirect immunofluorescence method for detection of infectious BK virus in urine
An indirect immunofluorescence (IF) method is described for the detection of infectious BK virus in urine within seven days in contrast to up to three months or longer using routine tissue culture. Virus is pelleted from the urine, inoculated onto pre-formed monolayers of human embryo lung (HEL) fib...
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| Veröffentlicht in: | Journal of virological methods 1989-12, Vol.26 (3), p.351-354 |
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| container_end_page | 354 |
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| container_issue | 3 |
| container_start_page | 351 |
| container_title | Journal of virological methods |
| container_volume | 26 |
| creator | Knowles, W.A. Woodroof, M. Porter, A.A. Gardner, S.D. |
| description | An indirect immunofluorescence (IF) method is described for the detection of infectious BK virus in urine within seven days in contrast to up to three months or longer using routine tissue culture. Virus is pelleted from the urine, inoculated onto pre-formed monolayers of human embryo lung (HEL) fibroblasts, and infected cells are detected in an indirect fluorescent antibody test using a human serum. The sensitivity of the IF method is 91%. Positive isolates may be confirmed as BK virus using specific rabbit antisera on the original inoculated cultures. Furthermore, a virus stock may be grown up by passage from the original culture fluids for further studies such as DNA analysis. As a broadly-reactive human serum is used for screening the cultures, other viruses which grow in HEL cells, such as CMV, may also be detected. |
| doi_str_mv | 10.1016/0166-0934(89)90118-3 |
| format | Article |
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Virus is pelleted from the urine, inoculated onto pre-formed monolayers of human embryo lung (HEL) fibroblasts, and infected cells are detected in an indirect fluorescent antibody test using a human serum. The sensitivity of the IF method is 91%. Positive isolates may be confirmed as BK virus using specific rabbit antisera on the original inoculated cultures. Furthermore, a virus stock may be grown up by passage from the original culture fluids for further studies such as DNA analysis. As a broadly-reactive human serum is used for screening the cultures, other viruses which grow in HEL cells, such as CMV, may also be detected.</description><subject>Biological and medical sciences</subject><subject>BK virus</subject><subject>BK Virus - isolation & purification</subject><subject>BK virus detection</subject><subject>Fibroblasts</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Indirect immunofluorescence method</subject><subject>Isolate typing</subject><subject>Microbiology</subject><subject>Polyomavirus - isolation & purification</subject><subject>Time Factors</subject><subject>Urine - microbiology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFTEUhoNY6rX6DxSyEKmL0WTyvRHai7alBRfqOsxNTjAyk9Rk5oL_3twP7tIuQnJynvfw8h6E3lDykRIqP7UjO2IYv9TmgyGU6o49QyuqlWnfmj9HqxPyAr2s9TchRCjGztF5L4QxvVih71cJx-RjATfjOE1LymFccoHqIDnAE8y_sschF-xhblDMCefQNGFfLBVf3-NtLO0RE15KTPAKnYVhrPD6eF-gn1-__Fjfdg_fbu7WVw-d41TNnfPUC2B8o5jUzDEFA4gegiaBSs-N6pnnqnecy556pUAGTYVSKmjZhzCwC_T-MPex5D8L1NlOsdkexyFBM2aVacpeiidBKjjVnKsG8gPoSq61QLCPJU5D-WspsbvQ7S5Ru0vUamP3oVvWZG-P85fNBP4kOqbc-u-O_aG6YQxlSC7WEyabRaZowz4fMGihbSMUW13cbeGwHutz_L-PfzASnR8</recordid><startdate>19891201</startdate><enddate>19891201</enddate><creator>Knowles, W.A.</creator><creator>Woodroof, M.</creator><creator>Porter, A.A.</creator><creator>Gardner, S.D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19891201</creationdate><title>An indirect immunofluorescence method for detection of infectious BK virus in urine</title><author>Knowles, W.A. ; Woodroof, M. ; Porter, A.A. ; Gardner, S.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-cd1d5e34b73683c37eae52ef80f16d49723d472c44621d77e6f815777f862ffa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Biological and medical sciences</topic><topic>BK virus</topic><topic>BK Virus - isolation & purification</topic><topic>BK virus detection</topic><topic>Fibroblasts</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Indirect immunofluorescence method</topic><topic>Isolate typing</topic><topic>Microbiology</topic><topic>Polyomavirus - isolation & purification</topic><topic>Time Factors</topic><topic>Urine - microbiology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knowles, W.A.</creatorcontrib><creatorcontrib>Woodroof, M.</creatorcontrib><creatorcontrib>Porter, A.A.</creatorcontrib><creatorcontrib>Gardner, S.D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knowles, W.A.</au><au>Woodroof, M.</au><au>Porter, A.A.</au><au>Gardner, S.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An indirect immunofluorescence method for detection of infectious BK virus in urine</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1989-12-01</date><risdate>1989</risdate><volume>26</volume><issue>3</issue><spage>351</spage><epage>354</epage><pages>351-354</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>An indirect immunofluorescence (IF) method is described for the detection of infectious BK virus in urine within seven days in contrast to up to three months or longer using routine tissue culture. Virus is pelleted from the urine, inoculated onto pre-formed monolayers of human embryo lung (HEL) fibroblasts, and infected cells are detected in an indirect fluorescent antibody test using a human serum. The sensitivity of the IF method is 91%. Positive isolates may be confirmed as BK virus using specific rabbit antisera on the original inoculated cultures. Furthermore, a virus stock may be grown up by passage from the original culture fluids for further studies such as DNA analysis. As a broadly-reactive human serum is used for screening the cultures, other viruses which grow in HEL cells, such as CMV, may also be detected.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2559925</pmid><doi>10.1016/0166-0934(89)90118-3</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1989-12, Vol.26 (3), p.351-354 |
| issn | 0166-0934 1879-0984 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences BK virus BK Virus - isolation & purification BK virus detection Fibroblasts Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Humans Indirect immunofluorescence method Isolate typing Microbiology Polyomavirus - isolation & purification Time Factors Urine - microbiology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Virology |
| title | An indirect immunofluorescence method for detection of infectious BK virus in urine |
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