Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y
G Jakab, E Droz, G Brigneti, D Baulcombe and P Malnoe Station Federale de Recherches en Production Vegetale de Changins, Nyon, Switzerland. A full-length cDNA clone of the potato virus Y (PVY) genome was obtained after cloning difficulties in Escherichia coli were overcome. These difficulties were m...
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| Veröffentlicht in: | Journal of general virology 1997-12, Vol.78 (12), p.3141-3145 |
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| container_title | Journal of general virology |
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| creator | Jakab, G Droz, E Brigneti, G Baulcombe, D Malnoe, P |
| description | G Jakab, E Droz, G Brigneti, D Baulcombe and P Malnoe
Station Federale de Recherches en Production Vegetale de Changins, Nyon, Switzerland.
A full-length cDNA clone of the potato virus Y (PVY) genome was obtained
after cloning difficulties in Escherichia coli were overcome. These
difficulties were mainly due to the expression of the CI gene from upstream
prokaryotic promoter-like elements within the PVY genome. To overcome this
problem, PVY cDNA was maintained in two subclones which were ligated before
infection. A plasmid in which these two fragments were contained could be
propagated in some E. coli strains but was unstable and yielded only low
levels of plasmid DNA. Replacement of the 35S promoter by the SP6 promoter
slightly increased the stability of the plasmid and its RNA transcripts
were infectious when capped with m7G(5')ppp(5')G. Using two inoculation
methods (mechanical or biolistic) the cDNA and its RNA transcript proved
infectious on three Nicotiana species and on Solanum tuberosum. |
| doi_str_mv | 10.1099/0022-1317-78-12-3141 |
| format | Article |
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Station Federale de Recherches en Production Vegetale de Changins, Nyon, Switzerland.
A full-length cDNA clone of the potato virus Y (PVY) genome was obtained
after cloning difficulties in Escherichia coli were overcome. These
difficulties were mainly due to the expression of the CI gene from upstream
prokaryotic promoter-like elements within the PVY genome. To overcome this
problem, PVY cDNA was maintained in two subclones which were ligated before
infection. A plasmid in which these two fragments were contained could be
propagated in some E. coli strains but was unstable and yielded only low
levels of plasmid DNA. Replacement of the 35S promoter by the SP6 promoter
slightly increased the stability of the plasmid and its RNA transcripts
were infectious when capped with m7G(5')ppp(5')G. Using two inoculation
methods (mechanical or biolistic) the cDNA and its RNA transcript proved
infectious on three Nicotiana species and on Solanum tuberosum.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-78-12-3141</identifier><identifier>PMID: 9400962</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>DNA, Complementary - genetics ; Genome, Viral ; Potyvirus - physiology ; Solanum tuberosum - virology ; Transcription, Genetic ; Virus Replication - genetics</subject><ispartof>Journal of general virology, 1997-12, Vol.78 (12), p.3141-3145</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-9ee0473cb5a1ff377ffb6ba31299e637e322db31ea43d543945e24372ee5cbff3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9400962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jakab, G</creatorcontrib><creatorcontrib>Droz, E</creatorcontrib><creatorcontrib>Brigneti, G</creatorcontrib><creatorcontrib>Baulcombe, D</creatorcontrib><creatorcontrib>Malnoe, P</creatorcontrib><title>Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>G Jakab, E Droz, G Brigneti, D Baulcombe and P Malnoe
Station Federale de Recherches en Production Vegetale de Changins, Nyon, Switzerland.
A full-length cDNA clone of the potato virus Y (PVY) genome was obtained
after cloning difficulties in Escherichia coli were overcome. These
difficulties were mainly due to the expression of the CI gene from upstream
prokaryotic promoter-like elements within the PVY genome. To overcome this
problem, PVY cDNA was maintained in two subclones which were ligated before
infection. A plasmid in which these two fragments were contained could be
propagated in some E. coli strains but was unstable and yielded only low
levels of plasmid DNA. Replacement of the 35S promoter by the SP6 promoter
slightly increased the stability of the plasmid and its RNA transcripts
were infectious when capped with m7G(5')ppp(5')G. Using two inoculation
methods (mechanical or biolistic) the cDNA and its RNA transcript proved
infectious on three Nicotiana species and on Solanum tuberosum.</description><subject>DNA, Complementary - genetics</subject><subject>Genome, Viral</subject><subject>Potyvirus - physiology</subject><subject>Solanum tuberosum - virology</subject><subject>Transcription, Genetic</subject><subject>Virus Replication - genetics</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtLXDEUhUOx2NH2H7SQJ0EwNbdzYh5FexHECr2ATyEnszMTOScZk4zSx_7zZjqD-NanBPa31mavhdB7Rj8yqvUppZwTJpgi6owwTgST7BWaMdl3hDdgD82ekTfooJR7SpmUndpH-1pSqns-Q3-uogdXQ1oXHCJ-DI8J2zjf_mtOuGYbi8thVQv2OU3YYr8eRzJCXNQldpc359iNKQJOHt_-uiM3Pe1OGvX9KZSCI7icanA4lDTa-o9apWprav65Lb17i157OxZ4t3sP0c_Pn35cfCXX375cXZxfEyeVrkQDUKmEGzrLvBdKeT_0gxWMaw29UCA4nw-CgZVi3kmhZQdcCsUBOjc0xSE62vqucnpYQ6lmCsXBONoI7XqjmkJzyf4Lsl6wTlDZQLkF24mlZPBmlcNk82_DqNlUZDb5m03-Rp0Zxs2moib7sPNfDxPMn0W7Ttr8eDtfhsXyKWQwC4hTaEuGkExL7YXXXzLjmlU</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Jakab, G</creator><creator>Droz, E</creator><creator>Brigneti, G</creator><creator>Baulcombe, D</creator><creator>Malnoe, P</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y</title><author>Jakab, G ; Droz, E ; Brigneti, G ; Baulcombe, D ; Malnoe, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-9ee0473cb5a1ff377ffb6ba31299e637e322db31ea43d543945e24372ee5cbff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>DNA, Complementary - genetics</topic><topic>Genome, Viral</topic><topic>Potyvirus - physiology</topic><topic>Solanum tuberosum - virology</topic><topic>Transcription, Genetic</topic><topic>Virus Replication - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jakab, G</creatorcontrib><creatorcontrib>Droz, E</creatorcontrib><creatorcontrib>Brigneti, G</creatorcontrib><creatorcontrib>Baulcombe, D</creatorcontrib><creatorcontrib>Malnoe, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jakab, G</au><au>Droz, E</au><au>Brigneti, G</au><au>Baulcombe, D</au><au>Malnoe, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>78</volume><issue>12</issue><spage>3141</spage><epage>3145</epage><pages>3141-3145</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>G Jakab, E Droz, G Brigneti, D Baulcombe and P Malnoe
Station Federale de Recherches en Production Vegetale de Changins, Nyon, Switzerland.
A full-length cDNA clone of the potato virus Y (PVY) genome was obtained
after cloning difficulties in Escherichia coli were overcome. These
difficulties were mainly due to the expression of the CI gene from upstream
prokaryotic promoter-like elements within the PVY genome. To overcome this
problem, PVY cDNA was maintained in two subclones which were ligated before
infection. A plasmid in which these two fragments were contained could be
propagated in some E. coli strains but was unstable and yielded only low
levels of plasmid DNA. Replacement of the 35S promoter by the SP6 promoter
slightly increased the stability of the plasmid and its RNA transcripts
were infectious when capped with m7G(5')ppp(5')G. Using two inoculation
methods (mechanical or biolistic) the cDNA and its RNA transcript proved
infectious on three Nicotiana species and on Solanum tuberosum.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>9400962</pmid><doi>10.1099/0022-1317-78-12-3141</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of general virology, 1997-12, Vol.78 (12), p.3141-3145 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79459241 |
| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | DNA, Complementary - genetics Genome, Viral Potyvirus - physiology Solanum tuberosum - virology Transcription, Genetic Virus Replication - genetics |
| title | Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y |
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