Use of silica gel polymer for DNA extraction with organic solvents
Phenol and chloroform are the standard solvents used for DNA extraction. These solvents aid in the removal of protein and lipid from crude or partially purified cell extracts. Although the procedure is well established, the solvents are noxious, caustic, and unpleasant. We describe in this paper the...
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| Veröffentlicht in: | Analytical biochemistry 1989-11, Vol.183 (1), p.13-15 |
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| container_title | Analytical biochemistry |
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| creator | Tilzer, Lowell Thomas, Stanley Moreno, Ruben F. |
| description | Phenol and chloroform are the standard solvents used for DNA extraction. These solvents aid in the removal of protein and lipid from crude or partially purified cell extracts. Although the procedure is well established, the solvents are noxious, caustic, and unpleasant. We describe in this paper the use of a special blood collection tube to isolate the offensive organic solvents. With the use of silica gel polymer containing tubes, phenol, phenol:chloroform, or chloroform can be separated from the DNA containing aqueous phase in a rapid and safe manner. The method permits higher yields of DNA since the DNA is poured from the tube rather than aspirated with pipet. |
| doi_str_mv | 10.1016/0003-2697(89)90163-2 |
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These solvents aid in the removal of protein and lipid from crude or partially purified cell extracts. Although the procedure is well established, the solvents are noxious, caustic, and unpleasant. We describe in this paper the use of a special blood collection tube to isolate the offensive organic solvents. With the use of silica gel polymer containing tubes, phenol, phenol:chloroform, or chloroform can be separated from the DNA containing aqueous phase in a rapid and safe manner. The method permits higher yields of DNA since the DNA is poured from the tube rather than aspirated with pipet.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(89)90163-2</identifier><identifier>PMID: 2559624</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chemistry Techniques, Analytical - methods ; DNA - blood ; DNA - isolation & purification ; Dna, deoxyribonucleoproteins ; Fundamental and applied biological sciences. 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These solvents aid in the removal of protein and lipid from crude or partially purified cell extracts. Although the procedure is well established, the solvents are noxious, caustic, and unpleasant. We describe in this paper the use of a special blood collection tube to isolate the offensive organic solvents. With the use of silica gel polymer containing tubes, phenol, phenol:chloroform, or chloroform can be separated from the DNA containing aqueous phase in a rapid and safe manner. The method permits higher yields of DNA since the DNA is poured from the tube rather than aspirated with pipet.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chemistry Techniques, Analytical - methods</subject><subject>DNA - blood</subject><subject>DNA - isolation & purification</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>Fundamental and applied biological sciences. 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| ispartof | Analytical biochemistry, 1989-11, Vol.183 (1), p.13-15 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Techniques, Analytical - methods DNA - blood DNA - isolation & purification Dna, deoxyribonucleoproteins Fundamental and applied biological sciences. Psychology Nucleic acids Silica Gel Silicon Dioxide Solvents |
| title | Use of silica gel polymer for DNA extraction with organic solvents |
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