Kinetics of the inhibition of mitochondrial respiration by NO
The kinetics of the inhibition of mitochondrial respiration by NO was examined in isolated mitochondria (here obtained from rat brown adipose tissue). The K i of NO for the inhibition was ∼27 nM; the IC 50 of NO increased in proportion to the square of an increase in O 2 tension. The K m of O 2 for...
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| Veröffentlicht in: | FEBS letters 1997-11, Vol.417 (1), p.75-80 |
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| creator | Koivisto, Ari Matthias, Anita Bronnikov, Gennady Nedergaard, Jan |
| description | The kinetics of the inhibition of mitochondrial respiration by NO was examined in isolated mitochondria (here obtained from rat brown adipose tissue). The
K
i of NO for the inhibition was ∼27 nM; the IC
50 of NO increased in proportion to the square of an increase in O
2 tension. The
K
m of O
2 for respiration was ∼16 μM; in the presence of NO, the dependence of respiration on O
2 tension had a Hill coefficient of ∼2. The unusual kinetics is probably related to the ability of cytochrome
c oxidase to use 2 NO or 1 O
2 as electron acceptor. The interaction between NO and O
2 in the control of respiration could be described by the formula
V
O
2
(O
2, NO)=
V
O
2
max·([O
2]
2/((16 μM·(1+[NO]/27 nM))
2+[O
2]
2)). Thus, the kinetics is such that respiration in the presence of physiological levels of NO is very sensitive to decreasing O
2 tension. |
| doi_str_mv | 10.1016/S0014-5793(97)01258-1 |
| format | Article |
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K
i of NO for the inhibition was ∼27 nM; the IC
50 of NO increased in proportion to the square of an increase in O
2 tension. The
K
m of O
2 for respiration was ∼16 μM; in the presence of NO, the dependence of respiration on O
2 tension had a Hill coefficient of ∼2. The unusual kinetics is probably related to the ability of cytochrome
c oxidase to use 2 NO or 1 O
2 as electron acceptor. The interaction between NO and O
2 in the control of respiration could be described by the formula
V
O
2
(O
2, NO)=
V
O
2
max·([O
2]
2/((16 μM·(1+[NO]/27 nM))
2+[O
2]
2)). Thus, the kinetics is such that respiration in the presence of physiological levels of NO is very sensitive to decreasing O
2 tension.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(97)01258-1</identifier><identifier>PMID: 9395078</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adipose Tissue, Brown - metabolism ; Animals ; Brown fat mitochondria ; Cytochrome c oxidase ; Female ; Hypoxia ; Kinetics ; Mitochondria - drug effects ; Mitochondria - metabolism ; Nitric oxide ; Nitric Oxide - pharmacology ; Non-shivering thermogenesis ; Oxygen ; Oxygen - metabolism ; Oxygen Consumption - drug effects ; Rats ; Rats, Sprague-Dawley</subject><ispartof>FEBS letters, 1997-11, Vol.417 (1), p.75-80</ispartof><rights>1997 Federation of European Biochemical Societies</rights><rights>FEBS Letters 417 (1997) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4721-dcd6d3e1529c26b5a598c03e45e5ac51052238a9128cdb570798abb081ee22523</citedby><cites>FETCH-LOGICAL-c4721-dcd6d3e1529c26b5a598c03e45e5ac51052238a9128cdb570798abb081ee22523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2897%2901258-1$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(97)01258-1$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9395078$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koivisto, Ari</creatorcontrib><creatorcontrib>Matthias, Anita</creatorcontrib><creatorcontrib>Bronnikov, Gennady</creatorcontrib><creatorcontrib>Nedergaard, Jan</creatorcontrib><title>Kinetics of the inhibition of mitochondrial respiration by NO</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The kinetics of the inhibition of mitochondrial respiration by NO was examined in isolated mitochondria (here obtained from rat brown adipose tissue). The
K
i of NO for the inhibition was ∼27 nM; the IC
50 of NO increased in proportion to the square of an increase in O
2 tension. The
K
m of O
2 for respiration was ∼16 μM; in the presence of NO, the dependence of respiration on O
2 tension had a Hill coefficient of ∼2. The unusual kinetics is probably related to the ability of cytochrome
c oxidase to use 2 NO or 1 O
2 as electron acceptor. The interaction between NO and O
2 in the control of respiration could be described by the formula
V
O
2
(O
2, NO)=
V
O
2
max·([O
2]
2/((16 μM·(1+[NO]/27 nM))
2+[O
2]
2)). Thus, the kinetics is such that respiration in the presence of physiological levels of NO is very sensitive to decreasing O
2 tension.</description><subject>Adipose Tissue, Brown - metabolism</subject><subject>Animals</subject><subject>Brown fat mitochondria</subject><subject>Cytochrome c oxidase</subject><subject>Female</subject><subject>Hypoxia</subject><subject>Kinetics</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - pharmacology</subject><subject>Non-shivering thermogenesis</subject><subject>Oxygen</subject><subject>Oxygen - metabolism</subject><subject>Oxygen Consumption - drug effects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAQRS0EKqXwCZWyQrAI2E4c2wuEoGopoqILYG05zlQ1yqPYKah_Tx5Vt7Aazdw7d0YHoTHBNwST5PYNYxKHjMvoSvJrTCgTITlCQyJ4FEZxIo7R8GA5RWfef-KmF0QO0EBGkmEuhujuxZZQW-ODahXUawhsubaprW1VtpPC1pVZV2XmrM4DB35jne7EdBe8Ls_RyUrnHi72dYQ-ZtP3yTxcLJ-eJw-L0MSckjAzWZJFQBiVhiYp00wKgyOIGTBtGMGM0khoSagwWco45lLoNMWCAFDKaDRCl33uxlVfW_C1Kqw3kOe6hGrrFZdxk41bI-uNxlXeO1ipjbOFdjtFsGqxqQ6bapkoyVWHTZFmb7w_sE0LyA5be06NPu_1H5vD7n-hajZ9pJ3SCpJ34_bUfR8FDbBvC055Y6E0kFkHplZZZf949hd5xY85</recordid><startdate>19971103</startdate><enddate>19971103</enddate><creator>Koivisto, Ari</creator><creator>Matthias, Anita</creator><creator>Bronnikov, Gennady</creator><creator>Nedergaard, Jan</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971103</creationdate><title>Kinetics of the inhibition of mitochondrial respiration by NO</title><author>Koivisto, Ari ; Matthias, Anita ; Bronnikov, Gennady ; Nedergaard, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4721-dcd6d3e1529c26b5a598c03e45e5ac51052238a9128cdb570798abb081ee22523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adipose Tissue, Brown - metabolism</topic><topic>Animals</topic><topic>Brown fat mitochondria</topic><topic>Cytochrome c oxidase</topic><topic>Female</topic><topic>Hypoxia</topic><topic>Kinetics</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - pharmacology</topic><topic>Non-shivering thermogenesis</topic><topic>Oxygen</topic><topic>Oxygen - metabolism</topic><topic>Oxygen Consumption - drug effects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koivisto, Ari</creatorcontrib><creatorcontrib>Matthias, Anita</creatorcontrib><creatorcontrib>Bronnikov, Gennady</creatorcontrib><creatorcontrib>Nedergaard, Jan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koivisto, Ari</au><au>Matthias, Anita</au><au>Bronnikov, Gennady</au><au>Nedergaard, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of the inhibition of mitochondrial respiration by NO</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1997-11-03</date><risdate>1997</risdate><volume>417</volume><issue>1</issue><spage>75</spage><epage>80</epage><pages>75-80</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The kinetics of the inhibition of mitochondrial respiration by NO was examined in isolated mitochondria (here obtained from rat brown adipose tissue). The
K
i of NO for the inhibition was ∼27 nM; the IC
50 of NO increased in proportion to the square of an increase in O
2 tension. The
K
m of O
2 for respiration was ∼16 μM; in the presence of NO, the dependence of respiration on O
2 tension had a Hill coefficient of ∼2. The unusual kinetics is probably related to the ability of cytochrome
c oxidase to use 2 NO or 1 O
2 as electron acceptor. The interaction between NO and O
2 in the control of respiration could be described by the formula
V
O
2
(O
2, NO)=
V
O
2
max·([O
2]
2/((16 μM·(1+[NO]/27 nM))
2+[O
2]
2)). Thus, the kinetics is such that respiration in the presence of physiological levels of NO is very sensitive to decreasing O
2 tension.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9395078</pmid><doi>10.1016/S0014-5793(97)01258-1</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Elsevier ScienceDirect Journals Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adipose Tissue, Brown - metabolism Animals Brown fat mitochondria Cytochrome c oxidase Female Hypoxia Kinetics Mitochondria - drug effects Mitochondria - metabolism Nitric oxide Nitric Oxide - pharmacology Non-shivering thermogenesis Oxygen Oxygen - metabolism Oxygen Consumption - drug effects Rats Rats, Sprague-Dawley |
| title | Kinetics of the inhibition of mitochondrial respiration by NO |
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