Structure of the three β-tubulin-encoding genes of the unicellular alga, Polytomella agilis
The quadriflagellate, unicellular, colorless alga, Polytomella agilis, contains several distinct microtubule arrays. To study the genetic basis of microtubule heterogeneity in P. agilis, we characterized its tubulin(Tub)-encoding genes ( tub). The three β tub genes detected in blots of P. agilis DNA...
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| Veröffentlicht in: | Gene 1989-12, Vol.84 (2), p.345-358 |
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| creator | Conner, Timothy W. Thompson, Michael D. Silflow, Carolyn D. |
| description | The quadriflagellate, unicellular, colorless alga,
Polytomella agilis, contains several distinct microtubule arrays. To study the genetic basis of microtubule heterogeneity in
P. agilis, we characterized its tubulin(Tub)-encoding genes (
tub). The three β
tub genes detected in blots of
P. agilis DNA were isolated from a genomic library. The structure and organization of the genes were examined by restriction mapping and nucleotide (nt) sequencing. S1 nuclease protection studies showed that all three genes are expressed. The predicted amino acid (aa) sequences are more than 98% conserved with the
Chlamydomonas reinhardtii and
Volvox carteri β-Tubs, underscoring the close phylogenetic relationship of these species. Evolutionary divergence among the
P. agilis genes is demonstrated by differences in intron number, nt sequences in noncoding regions, and silent nt substitutions in the coding regions. However, the proteins encoded by the β1 and β3
tub genes are identical; the β2 gene product differs by one conservative aa substitution. These results are in striking contrast to the C-terminal aa diversity reported within β
tub gene families in animal, higher plant and fungal systems. The data support the hypothesis that those
tub genes whose products assemble into axonemal microtubules are subject to strong selection against nt substitutions causing aa replacements. |
| doi_str_mv | 10.1016/0378-1119(89)90509-X |
| format | Article |
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Polytomella agilis, contains several distinct microtubule arrays. To study the genetic basis of microtubule heterogeneity in
P. agilis, we characterized its tubulin(Tub)-encoding genes (
tub). The three β
tub genes detected in blots of
P. agilis DNA were isolated from a genomic library. The structure and organization of the genes were examined by restriction mapping and nucleotide (nt) sequencing. S1 nuclease protection studies showed that all three genes are expressed. The predicted amino acid (aa) sequences are more than 98% conserved with the
Chlamydomonas reinhardtii and
Volvox carteri β-Tubs, underscoring the close phylogenetic relationship of these species. Evolutionary divergence among the
P. agilis genes is demonstrated by differences in intron number, nt sequences in noncoding regions, and silent nt substitutions in the coding regions. However, the proteins encoded by the β1 and β3
tub genes are identical; the β2 gene product differs by one conservative aa substitution. These results are in striking contrast to the C-terminal aa diversity reported within β
tub gene families in animal, higher plant and fungal systems. The data support the hypothesis that those
tub genes whose products assemble into axonemal microtubules are subject to strong selection against nt substitutions causing aa replacements.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(89)90509-X</identifier><identifier>PMID: 2533130</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Bacteriophage lambda - genetics ; Base Sequence ; Biological and medical sciences ; Biological Evolution ; Chlorophyta - genetics ; Codon ; DNA - genetics ; DNA - isolation & purification ; Eukaryota - genetics ; evolution ; Fundamental and applied biological sciences. Psychology ; Fungi - genetics ; gene families ; Gene Library ; Genes ; Genes. Genome ; genomes ; intron excision ; Introns ; microtubules ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Multigene Family ; Phylogeny ; Plants - genetics ; polyadenylation signal ; Polytomella agilis ; quadriflagellate ; Recombinant DNA ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Single-Strand Specific DNA and RNA Endonucleases ; structural analysis ; tubulin ; Tubulin - genetics</subject><ispartof>Gene, 1989-12, Vol.84 (2), p.345-358</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-774027fde17d89bb5eb8f911a82c8e7cc1a2d73fe04f6e982b472b2c49affadb3</citedby><cites>FETCH-LOGICAL-c332t-774027fde17d89bb5eb8f911a82c8e7cc1a2d73fe04f6e982b472b2c49affadb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(89)90509-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6714445$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2533130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Conner, Timothy W.</creatorcontrib><creatorcontrib>Thompson, Michael D.</creatorcontrib><creatorcontrib>Silflow, Carolyn D.</creatorcontrib><title>Structure of the three β-tubulin-encoding genes of the unicellular alga, Polytomella agilis</title><title>Gene</title><addtitle>Gene</addtitle><description>The quadriflagellate, unicellular, colorless alga,
Polytomella agilis, contains several distinct microtubule arrays. To study the genetic basis of microtubule heterogeneity in
P. agilis, we characterized its tubulin(Tub)-encoding genes (
tub). The three β
tub genes detected in blots of
P. agilis DNA were isolated from a genomic library. The structure and organization of the genes were examined by restriction mapping and nucleotide (nt) sequencing. S1 nuclease protection studies showed that all three genes are expressed. The predicted amino acid (aa) sequences are more than 98% conserved with the
Chlamydomonas reinhardtii and
Volvox carteri β-Tubs, underscoring the close phylogenetic relationship of these species. Evolutionary divergence among the
P. agilis genes is demonstrated by differences in intron number, nt sequences in noncoding regions, and silent nt substitutions in the coding regions. However, the proteins encoded by the β1 and β3
tub genes are identical; the β2 gene product differs by one conservative aa substitution. These results are in striking contrast to the C-terminal aa diversity reported within β
tub gene families in animal, higher plant and fungal systems. The data support the hypothesis that those
tub genes whose products assemble into axonemal microtubules are subject to strong selection against nt substitutions causing aa replacements.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacteriophage lambda - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>Chlorophyta - genetics</subject><subject>Codon</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>Eukaryota - genetics</subject><subject>evolution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi - genetics</subject><subject>gene families</subject><subject>Gene Library</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>genomes</subject><subject>intron excision</subject><subject>Introns</subject><subject>microtubules</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Phylogeny</subject><subject>Plants - genetics</subject><subject>polyadenylation signal</subject><subject>Polytomella agilis</subject><subject>quadriflagellate</subject><subject>Recombinant DNA</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Single-Strand Specific DNA and RNA Endonucleases</subject><subject>structural analysis</subject><subject>tubulin</subject><subject>Tubulin - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9KHDEUh0NR7Nb6BhbmQsSCU_NvNsmNINKqILSggheFkMmcbFOyM2syEXwtH6TP1Ky77qUGQuCc7xx-fEFon-BvBJPpCWZC1oQQdSTVV4UbrOr7D2hCpFA1xkxuockG-Yg-pfQXl9M0dAft0IYxwvAE_b4ZY7ZjjlANrhr_QLkRoPr3XI-5zcH3NfR26Hw_q2bQQ3rFcu8thJCDiZUJM3Nc_RrC0zjMS9FUZuaDT5_RtjMhwd763UV3P77fnl_W1z8vrs7PrmvLGB1rITimwnVARCdV2zbQSqcIMZJaCcJaYmgnmAPM3RSUpC0XtKWWK-Oc6Vq2iw5XexdxeMiQRj33aZnO9DDkpIXinHCu3gVJw6eYElFAvgJtHFKK4PQi-rmJT5pgvbSvl2r1Uq2WSr_Y1_dl7Mt6f27n0G2G1rpL_2DdN8ma4KLprU8bbCpKSt4U7HSFQZH26CHqZH35Buh8BDvqbvBv5_gPFlqicg</recordid><startdate>19891214</startdate><enddate>19891214</enddate><creator>Conner, Timothy W.</creator><creator>Thompson, Michael D.</creator><creator>Silflow, Carolyn D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19891214</creationdate><title>Structure of the three β-tubulin-encoding genes of the unicellular alga, Polytomella agilis</title><author>Conner, Timothy W. ; Thompson, Michael D. ; Silflow, Carolyn D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-774027fde17d89bb5eb8f911a82c8e7cc1a2d73fe04f6e982b472b2c49affadb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacteriophage lambda - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>Chlorophyta - genetics</topic><topic>Codon</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>Eukaryota - genetics</topic><topic>evolution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi - genetics</topic><topic>gene families</topic><topic>Gene Library</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>genomes</topic><topic>intron excision</topic><topic>Introns</topic><topic>microtubules</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Phylogeny</topic><topic>Plants - genetics</topic><topic>polyadenylation signal</topic><topic>Polytomella agilis</topic><topic>quadriflagellate</topic><topic>Recombinant DNA</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Single-Strand Specific DNA and RNA Endonucleases</topic><topic>structural analysis</topic><topic>tubulin</topic><topic>Tubulin - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Conner, Timothy W.</creatorcontrib><creatorcontrib>Thompson, Michael D.</creatorcontrib><creatorcontrib>Silflow, Carolyn D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Conner, Timothy W.</au><au>Thompson, Michael D.</au><au>Silflow, Carolyn D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure of the three β-tubulin-encoding genes of the unicellular alga, Polytomella agilis</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1989-12-14</date><risdate>1989</risdate><volume>84</volume><issue>2</issue><spage>345</spage><epage>358</epage><pages>345-358</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The quadriflagellate, unicellular, colorless alga,
Polytomella agilis, contains several distinct microtubule arrays. To study the genetic basis of microtubule heterogeneity in
P. agilis, we characterized its tubulin(Tub)-encoding genes (
tub). The three β
tub genes detected in blots of
P. agilis DNA were isolated from a genomic library. The structure and organization of the genes were examined by restriction mapping and nucleotide (nt) sequencing. S1 nuclease protection studies showed that all three genes are expressed. The predicted amino acid (aa) sequences are more than 98% conserved with the
Chlamydomonas reinhardtii and
Volvox carteri β-Tubs, underscoring the close phylogenetic relationship of these species. Evolutionary divergence among the
P. agilis genes is demonstrated by differences in intron number, nt sequences in noncoding regions, and silent nt substitutions in the coding regions. However, the proteins encoded by the β1 and β3
tub genes are identical; the β2 gene product differs by one conservative aa substitution. These results are in striking contrast to the C-terminal aa diversity reported within β
tub gene families in animal, higher plant and fungal systems. The data support the hypothesis that those
tub genes whose products assemble into axonemal microtubules are subject to strong selection against nt substitutions causing aa replacements.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2533130</pmid><doi>10.1016/0378-1119(89)90509-X</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1989-12, Vol.84 (2), p.345-358 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79441449 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Animals Bacteriophage lambda - genetics Base Sequence Biological and medical sciences Biological Evolution Chlorophyta - genetics Codon DNA - genetics DNA - isolation & purification Eukaryota - genetics evolution Fundamental and applied biological sciences. Psychology Fungi - genetics gene families Gene Library Genes Genes. Genome genomes intron excision Introns microtubules Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family Phylogeny Plants - genetics polyadenylation signal Polytomella agilis quadriflagellate Recombinant DNA Restriction Mapping Sequence Homology, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases structural analysis tubulin Tubulin - genetics |
| title | Structure of the three β-tubulin-encoding genes of the unicellular alga, Polytomella agilis |
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