Construction and properties of an Epstein-Barr-virus-derived cDNA expression vector for human cells

A cDNA expression vector containing the element oriP and the sequence encoding the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) as well as the hygromycin B-resistance dominant marker gene has been constructed. Its characteristics have been compared to a similar vector lacking the EBV sequence...

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Veröffentlicht in:	Gene 1989-12, Vol.84 (2), p.407-417
Hauptverfasser:	Belt, Peter B.G.M., Groeneveld, Herman, Teubel, Wilma J., van de Putte, Pieter, Backendorf, Claude
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Viral - genetics Biological and medical sciences Biotechnology Cell Line, Transformed cloning vectors Cloning, Molecular DNA - isolation & purification DNA Replication DNA, Viral - genetics episomal cDNA expression Epstein-Barr Virus Nuclear Antigens Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Genetic Vectors HeLa Cells Herpesvirus 4, Human - genetics Humans hygromycin resistance marker Methods. Procedures. Technologies oriP Plasmids - genetics Recombinant DNA RNA - isolation & purification Simian virus 40 - genetics SV40-transformed xeroderma pigmentosum Transfection Tumor Cells, Cultured
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