A marked diurnal rhythm of melatonin ML1A receptor mRNA expression in the suprachiasmatic nucleus
Melatonin (ML1A) receptor binding in the suprachiasmatic nucleus (SCN) exhibits a diurnal rhythm, with significant increases during the daytime. In order to determine whether the diurnal variation in binding is reflected by a cycling of melatonin ML1A receptor mRNA levels, we utilized a semiquantita...
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| Veröffentlicht in: | Brain research. Molecular brain research. 1997-10, Vol.49 (1-2), p.303-306 |
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| container_title | Brain research. Molecular brain research. |
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| creator | NEU, J. M NILES, L. P |
| description | Melatonin (ML1A) receptor binding in the suprachiasmatic nucleus (SCN) exhibits a diurnal rhythm, with significant increases during the daytime. In order to determine whether the diurnal variation in binding is reflected by a cycling of melatonin ML1A receptor mRNA levels, we utilized a semiquantitative reverse transcriptase-polymerase chain reaction procedure to examine receptor expression throughout the light-dark cycle. Here we report the first evidence of a significant diurnal variation in ML1A receptor mRNA levels within the SCN. Furthermore, this mRNA expression occurs approximately 3 h prior to, but is tightly correlated to the diurnal rhythm in high-affinity melatonin binding in the SCN. |
| doi_str_mv | 10.1016/S0169-328X(97)00218-0 |
| format | Article |
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P</creatorcontrib><title>A marked diurnal rhythm of melatonin ML1A receptor mRNA expression in the suprachiasmatic nucleus</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>Melatonin (ML1A) receptor binding in the suprachiasmatic nucleus (SCN) exhibits a diurnal rhythm, with significant increases during the daytime. In order to determine whether the diurnal variation in binding is reflected by a cycling of melatonin ML1A receptor mRNA levels, we utilized a semiquantitative reverse transcriptase-polymerase chain reaction procedure to examine receptor expression throughout the light-dark cycle. Here we report the first evidence of a significant diurnal variation in ML1A receptor mRNA levels within the SCN. Furthermore, this mRNA expression occurs approximately 3 h prior to, but is tightly correlated to the diurnal rhythm in high-affinity melatonin binding in the SCN.</description><subject>Actins - biosynthesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Circadian Rhythm</subject><subject>Darkness</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Light</subject><subject>Male</subject><subject>Melatonin - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Cell Surface - biosynthesis</subject><subject>Receptors, Cytoplasmic and Nuclear - biosynthesis</subject><subject>Receptors, Melatonin</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Suprachiasmatic Nucleus - metabolism</subject><subject>Transcription, Genetic</subject><subject>Vertebrates: endocrinology</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtKAzEUhoMotVYfQchCRBejydySLEvxBlXBC7gLp7kw0bmZzIB9e6ft0M05i__7z4EPoXNKbiih-e37MESUxPzrSrBrQmLKI3KAppSzOMpFSg_RdI8co5MQvgkhlFM6QRORcMZFMkUwxxX4H6Oxdr2vocS-WHdFhRuLK1NC19Suxs9LOsfeKNN2jcfV28scm7_WmxBcU-MB6AqDQ996UIWDUEHnFK57VZo-nKIjC2UwZ-Oeoc_7u4_FY7R8fXhazJeRivOsi1gqDFiRkJVSQjNgyhLgwIjKLAebEp1pq3WumCZC2IQOTMoylZqMQMpXyQxd7u62vvntTehk5YIyZQm1afogmUgTzvNsALMdqHwTgjdWtt4NEtaSErlRK7dq5cabFExu1Uoy9M7HB_2qMnrfGl0O-cWYQ1BQWg-1cmGPxTxjMY-Tfx5UgzQ</recordid><startdate>19971003</startdate><enddate>19971003</enddate><creator>NEU, J. 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Psychology</topic><topic>Gene Expression Regulation</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Light</topic><topic>Male</topic><topic>Melatonin - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Cell Surface - biosynthesis</topic><topic>Receptors, Cytoplasmic and Nuclear - biosynthesis</topic><topic>Receptors, Melatonin</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Suprachiasmatic Nucleus - metabolism</topic><topic>Transcription, Genetic</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NEU, J. M</creatorcontrib><creatorcontrib>NILES, L. 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Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>1997-10-03</date><risdate>1997</risdate><volume>49</volume><issue>1-2</issue><spage>303</spage><epage>306</epage><pages>303-306</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>Melatonin (ML1A) receptor binding in the suprachiasmatic nucleus (SCN) exhibits a diurnal rhythm, with significant increases during the daytime. In order to determine whether the diurnal variation in binding is reflected by a cycling of melatonin ML1A receptor mRNA levels, we utilized a semiquantitative reverse transcriptase-polymerase chain reaction procedure to examine receptor expression throughout the light-dark cycle. Here we report the first evidence of a significant diurnal variation in ML1A receptor mRNA levels within the SCN. 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| ispartof | Brain research. Molecular brain research., 1997-10, Vol.49 (1-2), p.303-306 |
| issn | 0169-328X 1872-6941 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Actins - biosynthesis Animals Biological and medical sciences Circadian Rhythm Darkness Fundamental and applied biological sciences. Psychology Gene Expression Regulation Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Light Male Melatonin - metabolism Polymerase Chain Reaction Rats Rats, Sprague-Dawley Receptors, Cell Surface - biosynthesis Receptors, Cytoplasmic and Nuclear - biosynthesis Receptors, Melatonin RNA, Messenger - biosynthesis Suprachiasmatic Nucleus - metabolism Transcription, Genetic Vertebrates: endocrinology |
| title | A marked diurnal rhythm of melatonin ML1A receptor mRNA expression in the suprachiasmatic nucleus |
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