Two distinct immunogenic epitopes on the α chain of human T cell antigen receptor
Monoclonal antibodies (MAb) to human T Cell Antigen Receptor (TCR) have been used to study the structure and function of TCR. Using purified alpha/beta heterodimeric protein, we have generated two MAb against human TCR alpha protein. The two MAb, alpha F1 and alpha F2, recognized amino acid residues...
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| Veröffentlicht in: | Hybridoma 1989-12, Vol.8 (6), p.577-588 |
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| creator | HENRY, L WEI-TAO TIAN RITTERSHAUS, C JONE-LONG KO MARSH, H. C. JR IP, S. H |
| description | Monoclonal antibodies (MAb) to human T Cell Antigen Receptor (TCR) have been used to study the structure and function of TCR. Using purified alpha/beta heterodimeric protein, we have generated two MAb against human TCR alpha protein. The two MAb, alpha F1 and alpha F2, recognized amino acid residues 141-159 and 212-231 of the constant region of the alpha chain TCR. Although neither MAb reacted with viable T cells, both antibodies immunoprecipitated TCR alpha/beta heterodimer from HPB-ALL, Jurkat, PBL and a 32 kDa in vitro translation product of alpha chain cDNA. These antibodies have been shown to be useful in the immunohistochemical staining of human tissues. These two MAb, together with other anti-framwork MAb to TCR, should provide valuable reagents in the study of TCR and clinical classification of T cell lineage neoplasms. |
| doi_str_mv | 10.1089/hyb.1989.8.577 |
| format | Article |
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C. JR</creatorcontrib><creatorcontrib>IP, S. H</creatorcontrib><title>Two distinct immunogenic epitopes on the α chain of human T cell antigen receptor</title><title>Hybridoma</title><addtitle>Hybridoma</addtitle><description>Monoclonal antibodies (MAb) to human T Cell Antigen Receptor (TCR) have been used to study the structure and function of TCR. Using purified alpha/beta heterodimeric protein, we have generated two MAb against human TCR alpha protein. The two MAb, alpha F1 and alpha F2, recognized amino acid residues 141-159 and 212-231 of the constant region of the alpha chain TCR. Although neither MAb reacted with viable T cells, both antibodies immunoprecipitated TCR alpha/beta heterodimer from HPB-ALL, Jurkat, PBL and a 32 kDa in vitro translation product of alpha chain cDNA. These antibodies have been shown to be useful in the immunohistochemical staining of human tissues. These two MAb, together with other anti-framwork MAb to TCR, should provide valuable reagents in the study of TCR and clinical classification of T cell lineage neoplasms.</description><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cross Reactions</subject><subject>Epitopes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Molecular immunology</subject><subject>Monoclonal antibodies</subject><subject>Receptors, Antigen, T-Cell - immunology</subject><subject>Receptors, Antigen, T-Cell, alpha-beta</subject><subject>T-Lymphocytes - immunology</subject><issn>0272-457X</issn><issn>2168-7897</issn><issn>0272-457X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9r3DAQxUVpSLZpr70FdCi52ZVk_fMxhDYtBAJlC7kJaTzuqqwlx7IJ-Vj5Iv1M8ZIl1x6GOczvPd48Qj5zVnNm26-7p1Dz1ra1rZUx78hGcG0rY1vznmyYMKKSytyfkQ-l_GWMKSHNKTkV0op1NuTX9jHTLpY5JphpHIYl5T-YIlAc45xHLDQnOu-Q_numsPMx0dzT3TL4RLcUcL-nPs1xldAJAcc5Tx_JSe_3BT8d9zn5_f3b9vpHdXt38_P66rYCodVcIWoE1auAgdmgodOy6zrdBNCytbYNfv0HeuZbEMoCB-Co-j4wEZi2jWzOyeWr7zjlhwXL7IZYDol8wrwUZ1rZaM7Nf0GuJDtYrmD9CsKUS5mwd-MUBz89Oc7coW23tu0ObTvr1nSr4OLovIQBuzf8WO96_3K8-wJ-308-QSxvmNZaSCGaFxlviYU</recordid><startdate>198912</startdate><enddate>198912</enddate><creator>HENRY, L</creator><creator>WEI-TAO TIAN</creator><creator>RITTERSHAUS, C</creator><creator>JONE-LONG KO</creator><creator>MARSH, H. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Molecular immunology</topic><topic>Monoclonal antibodies</topic><topic>Receptors, Antigen, T-Cell - immunology</topic><topic>Receptors, Antigen, T-Cell, alpha-beta</topic><topic>T-Lymphocytes - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>HENRY, L</creatorcontrib><creatorcontrib>WEI-TAO TIAN</creatorcontrib><creatorcontrib>RITTERSHAUS, C</creatorcontrib><creatorcontrib>JONE-LONG KO</creatorcontrib><creatorcontrib>MARSH, H. C. JR</creatorcontrib><creatorcontrib>IP, S. 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H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two distinct immunogenic epitopes on the α chain of human T cell antigen receptor</atitle><jtitle>Hybridoma</jtitle><addtitle>Hybridoma</addtitle><date>1989-12</date><risdate>1989</risdate><volume>8</volume><issue>6</issue><spage>577</spage><epage>588</epage><pages>577-588</pages><issn>0272-457X</issn><eissn>2168-7897</eissn><eissn>0272-457X</eissn><coden>HYBRDY</coden><abstract>Monoclonal antibodies (MAb) to human T Cell Antigen Receptor (TCR) have been used to study the structure and function of TCR. Using purified alpha/beta heterodimeric protein, we have generated two MAb against human TCR alpha protein. The two MAb, alpha F1 and alpha F2, recognized amino acid residues 141-159 and 212-231 of the constant region of the alpha chain TCR. Although neither MAb reacted with viable T cells, both antibodies immunoprecipitated TCR alpha/beta heterodimer from HPB-ALL, Jurkat, PBL and a 32 kDa in vitro translation product of alpha chain cDNA. These antibodies have been shown to be useful in the immunohistochemical staining of human tissues. These two MAb, together with other anti-framwork MAb to TCR, should provide valuable reagents in the study of TCR and clinical classification of T cell lineage neoplasms.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>2482248</pmid><doi>10.1089/hyb.1989.8.577</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0272-457X |
| ispartof | Hybridoma, 1989-12, Vol.8 (6), p.577-588 |
| issn | 0272-457X 2168-7897 0272-457X |
| language | eng |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Antibodies, immunoglobulins Antibodies, Monoclonal Antibody Specificity Biological and medical sciences Cell Line Cross Reactions Epitopes Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunohistochemistry Molecular immunology Monoclonal antibodies Receptors, Antigen, T-Cell - immunology Receptors, Antigen, T-Cell, alpha-beta T-Lymphocytes - immunology |
| title | Two distinct immunogenic epitopes on the α chain of human T cell antigen receptor |
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