Autoactivation of human recombinant coagulation factor VII

Single-chain human recombinant factor VII produced by transfected baby hamster kidney cells was purified to homogeneity in the presence of benzamidine. The amidolytic activity of single-chain recombinant factor VII with a peptidylnitroanilide substrate, methoxycarbonyl-D-cyclohexanylglycyl-L-arginin...

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Veröffentlicht in:	Biochemistry (Easton) 1989-11, Vol.28 (24), p.9331-9336
Hauptverfasser:	Pedersen, Anders H, Lund-Hansen, Torben, Bisgaard-Frantzen, Henrik, Olsen, Frank, Petersen, Lars C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Benzamidines - pharmacology Biological and medical sciences Blood coagulation. Blood cells Blotting, Western Calcium Chloride - pharmacology Cell Line Chromatography, High Pressure Liquid Coagulation factors Factor VII - isolation & purification Factor VII - metabolism Factor VIIa - metabolism Fundamental and applied biological sciences. Psychology Humans Kinetics man Molecular and cellular biology Polylysine - pharmacology Recombinant Proteins - metabolism Transfection Trypsin Inhibitors - pharmacology
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