Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum
Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. The...
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| Veröffentlicht in: | Cell and tissue research 1989-12, Vol.258 (3), p.555-561 |
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| container_end_page | 561 |
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| container_title | Cell and tissue research |
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| creator | RUTTEN, M. J GARRISON, R. G MOORE, C. D FISKIN, A. M CHEUNG, L. Y |
| description | Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific localization of alkaline phosphatase activity to the plasma membrane. Treatment of the tissues with levamisole (an alkaline phosphatase inhibitor) did not markedly reduce the specific alkaline phosphatase activity. Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis. |
| doi_str_mv | 10.1007/BF00218867 |
| format | Article |
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The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/BF00218867</identifier><identifier>PMID: 2611860</identifier><identifier>CODEN: CTSRCS</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Alkaline Phosphatase - analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Endoplasmic Reticulum - enzymology ; Enzymes and enzyme inhibitors ; Female ; Fundamental and applied biological sciences. Psychology ; Gastric Mucosa - cytology ; Gastric Mucosa - enzymology ; Gastric Mucosa - metabolism ; Gastrins - metabolism ; Golgi Apparatus - enzymology ; Histocytochemistry ; Hydrolases ; Intracellular Membranes - enzymology ; Male ; Microscopy, Electron ; Necturus - metabolism ; Necturus maculosus - anatomy & histology ; Necturus maculosus - metabolism ; Pyloric Antrum</subject><ispartof>Cell and tissue research, 1989-12, Vol.258 (3), p.555-561</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6706075$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2611860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RUTTEN, M. 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Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis.</description><subject>Alkaline Phosphatase - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Endoplasmic Reticulum - enzymology</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastric Mucosa - cytology</subject><subject>Gastric Mucosa - enzymology</subject><subject>Gastric Mucosa - metabolism</subject><subject>Gastrins - metabolism</subject><subject>Golgi Apparatus - enzymology</subject><subject>Histocytochemistry</subject><subject>Hydrolases</subject><subject>Intracellular Membranes - enzymology</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Necturus - metabolism</subject><subject>Necturus maculosus - anatomy & histology</subject><subject>Necturus maculosus - metabolism</subject><subject>Pyloric Antrum</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LwzAUx4Moc04v3oUcxINQfUnaJD3q2KYw9KLgraRpyqptM5P0MP96Myzz8n7wPnx5fBC6JHBHAMT94xKAEim5OEJTkjKagBTyGE2BAU0E5x-n6Mz7TwCScp5P0IRyQiSHKSoXrdHB2T7Ru2D1xnSNVi1ubazNjwqN7bGtsWq_4t4bvN1Yv92ooLzBweIV1qZt_R55iTmDGzzulB5a6-Ok-uCG7hyd1Kr15mLsM_S-XLzNn5L16-p5_rBONBUQkorJKqOpIkBFRjUvhTCplkZmOWOVMlKIugROK2ZIDkxyBqlkpagN0TVjNZuhm7_crbPfg_Gh6Bq_f0_1xg6-EHlKc05YBG__QO2s987UxdY1nXK7gkCxF1r8C43w1Zg6lJ2pDuhoMN6vx7vy0VntVK8bf8C4AA4iY79ngX1y</recordid><startdate>19891201</startdate><enddate>19891201</enddate><creator>RUTTEN, M. 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Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-d38d524a102752c6b77e4c8e85933dae877fb062d3e19038630483b7fe1cf33f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Alkaline Phosphatase - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Endoplasmic Reticulum - enzymology</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastric Mucosa - cytology</topic><topic>Gastric Mucosa - enzymology</topic><topic>Gastric Mucosa - metabolism</topic><topic>Gastrins - metabolism</topic><topic>Golgi Apparatus - enzymology</topic><topic>Histocytochemistry</topic><topic>Hydrolases</topic><topic>Intracellular Membranes - enzymology</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Necturus - metabolism</topic><topic>Necturus maculosus - anatomy & histology</topic><topic>Necturus maculosus - metabolism</topic><topic>Pyloric Antrum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RUTTEN, M. J</creatorcontrib><creatorcontrib>GARRISON, R. G</creatorcontrib><creatorcontrib>MOORE, C. D</creatorcontrib><creatorcontrib>FISKIN, A. M</creatorcontrib><creatorcontrib>CHEUNG, L. 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Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>1989-12-01</date><risdate>1989</risdate><volume>258</volume><issue>3</issue><spage>555</spage><epage>561</epage><pages>555-561</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><coden>CTSRCS</coden><abstract>Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific localization of alkaline phosphatase activity to the plasma membrane. Treatment of the tissues with levamisole (an alkaline phosphatase inhibitor) did not markedly reduce the specific alkaline phosphatase activity. Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2611860</pmid><doi>10.1007/BF00218867</doi><tpages>7</tpages></addata></record> |
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| ispartof | Cell and tissue research, 1989-12, Vol.258 (3), p.555-561 |
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| language | eng |
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| subjects | Alkaline Phosphatase - analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Endoplasmic Reticulum - enzymology Enzymes and enzyme inhibitors Female Fundamental and applied biological sciences. Psychology Gastric Mucosa - cytology Gastric Mucosa - enzymology Gastric Mucosa - metabolism Gastrins - metabolism Golgi Apparatus - enzymology Histocytochemistry Hydrolases Intracellular Membranes - enzymology Male Microscopy, Electron Necturus - metabolism Necturus maculosus - anatomy & histology Necturus maculosus - metabolism Pyloric Antrum |
| title | Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum |
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