Cloning and characterization of a relA/spoT homologue from Bacillus subtilis

A PCR‐amplified DNA fragment of the relA gene from genomic Bacillus subtilis DNA was used to isolate the entire relA/spoT homologue and two adjacent open reading frames (ORFs) from a λ ZAP Express library. The relA gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (...

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Veröffentlicht in:	Molecular microbiology 1997-10, Vol.26 (1), p.65-79
Hauptverfasser:	Wendrich, Thomas M., Marahiel, Mohamed A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacillus subtilis - enzymology Bacillus subtilis - genetics Bacillus subtilis - growth & development Bacterial Proteins - chemistry Base Sequence Blotting, Southern Blotting, Western Cloning, Molecular DNA Primers Electrophoresis, Gel, Two-Dimensional Guanosine Pentaphosphate - metabolism Ligases - chemistry Ligases - genetics Ligases - metabolism Molecular Sequence Data Pyrophosphatases - chemistry Pyrophosphatases - genetics Pyrophosphatases - metabolism Sequence Analysis, DNA Sequence Deletion Sequence Homology, Amino Acid Transcription, Genetic - genetics
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