A temperature upshift induces initiation of replication at oriC on the Escherichia coli chromosome
A temperature upshift of 10 or more degrees in the growth temperature of a bacterial culture causes induction of extra rounds of chromosome replication. This heat‐induced replication (HIR) initiates at oriC, is transitory, requires RNase H1 and RecA proteins and requires neither RNA polymerase activ...
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| Veröffentlicht in: | Molecular microbiology 1997-10, Vol.26 (1), p.133-144 |
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| container_title | Molecular microbiology |
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| creator | Botello, Emilia Jiménez‐Sánchez, Alfonso |
| description | A temperature upshift of 10 or more degrees in the growth temperature of a bacterial culture causes induction of extra rounds of chromosome replication. This heat‐induced replication (HIR) initiates at oriC, is transitory, requires RNase H1 and RecA proteins and requires neither RNA polymerase activity nor de novo protein synthesis. The number of origins activated by heat is growth rate and temperature differential dependent. An origin activation higher than 20% increases the DNA:mass ratio around twofold, and this value is kept constant for the subsequent generations of growth at 41°C. We have also shown that HIR is neither related to SDR nor induced by the heat shock response. We suggest that a thermodynamic alteration of oriC structure or of membrane fluidity could explain the observed HIR. |
| doi_str_mv | 10.1046/j.1365-2958.1997.5621924.x |
| format | Article |
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This heat‐induced replication (HIR) initiates at oriC, is transitory, requires RNase H1 and RecA proteins and requires neither RNA polymerase activity nor de novo protein synthesis. The number of origins activated by heat is growth rate and temperature differential dependent. An origin activation higher than 20% increases the DNA:mass ratio around twofold, and this value is kept constant for the subsequent generations of growth at 41°C. We have also shown that HIR is neither related to SDR nor induced by the heat shock response. 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| ispartof | Molecular microbiology, 1997-10, Vol.26 (1), p.133-144 |
| issn | 0950-382X 1365-2958 |
| language | eng |
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| source | MEDLINE; Wiley Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Free Full-Text Journals in Chemistry |
| subjects | Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacterial Proteins - metabolism DNA Replication DNA, Bacterial - biosynthesis Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli Proteins Heat-Shock Proteins - genetics Heat-Shock Proteins - metabolism Heat-Shock Response Hot Temperature Isopropyl Thiogalactoside - pharmacology Lac Repressors Plasmids Rec A Recombinases - metabolism Replication Origin Repressor Proteins - genetics Ribonuclease H - metabolism Rifampin - pharmacology Sigma Factor - genetics Sigma Factor - metabolism Transcription Factors |
| title | A temperature upshift induces initiation of replication at oriC on the Escherichia coli chromosome |
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