Cloning of Novel Trinucleotide-Repeat (CAG) Containing Genes in Mouse Brain
CAG trinucleotide repeat (CTR) sequence often appears in mammalian genome including transcription-regulatory protein and homeobox genes. Its expansion is associated with six genetic disorders in human. To identify novel CTR-containing genes expressed in mouse brain, a brain cDNA library was screened...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1997-11, Vol.240 (1), p.239-243 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 243 |
|---|---|
| container_issue | 1 |
| container_start_page | 239 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 240 |
| creator | Kim, Sun Jung Shon, Bo Hwa Kang, Joo Hyun Hahm, Kyung-Soo Yoo, Ook Joon Park, Young Sik Lee, Kyung-Kwang |
| description | CAG trinucleotide repeat (CTR) sequence often appears in mammalian genome including transcription-regulatory protein and homeobox genes. Its expansion is associated with six genetic disorders in human. To identify novel CTR-containing genes expressed in mouse brain, a brain cDNA library was screened using an oligonucleotide, (CTG)10. Eight clones were novel mouse genes and they were sequenced on both strands. The size of the cloned DNA ranged from 0.5 to 2.1 kb. The number of the CAG repeats in the clones ranged from 6 to 25. The inserts of the clones were analyzed for open reading frames and the peptide sequences were used for a GenBank homology search. Of the clones, one (CAG-6) shared 13 consecutive identical amino acid residues with the OB-cadherin gene, a member of cadherin family. CAG-14 showed high homology (657 nucleotides identity in 1022 nucleotides; 64%) with the 3′-untranslated region of rat leukocyte common antigen-related (LAR) tyrosine phosphatase receptor. All the 8 clones were originated from mouse DNA as judged by Southern blot analysis of mouse genomic DNA. The expression of the clones in mouse brain was addressed by RT-PCR and 4 clones showed specific expression. |
| doi_str_mv | 10.1006/bbrc.1997.7643 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79426903</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X97976439</els_id><sourcerecordid>79426903</sourcerecordid><originalsourceid>FETCH-LOGICAL-c339t-ed6bbd0eefc0f9345b702fe8e0097e63f4f6a14423635e3690f19f0fffe015ab3</originalsourceid><addsrcrecordid>eNp1kNFLwzAQh4Moc05ffRP6JPrQemmydHnUolOcCjLBt9CmF4l0zUzagf-9rRu--XRw992Pu4-QUwoJBRBXZel1QqXMkkxwtkfGFCTEKQW-T8bQE3Eq6fshOQrhE4BSLuSIjCQTmaTZmDzmtWts8xE5Ez27DdbR0tum0zW61lYYv-Iaiza6yK_nl1Humrawv_gcGwyRbaIn1wWMbnzfPyYHpqgDnuzqhLzd3S7z-3jxMn_IrxexZky2MVaiLCtANBqMZHxaZpAanCGAzFAww40oKOcpE2yKTEgwVBowxiDQaVGyCTnf5q69--owtGplg8a6Lhrsr1GZ5Gm_xXow2YLauxA8GrX2dlX4b0VBDfbUYE8N9tRgr1842yV35QqrP3ynq5_PtnPs39tY9Cpoi43GynrUraqc_S_6B9ohfZI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79426903</pqid></control><display><type>article</type><title>Cloning of Novel Trinucleotide-Repeat (CAG) Containing Genes in Mouse Brain</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Kim, Sun Jung ; Shon, Bo Hwa ; Kang, Joo Hyun ; Hahm, Kyung-Soo ; Yoo, Ook Joon ; Park, Young Sik ; Lee, Kyung-Kwang</creator><creatorcontrib>Kim, Sun Jung ; Shon, Bo Hwa ; Kang, Joo Hyun ; Hahm, Kyung-Soo ; Yoo, Ook Joon ; Park, Young Sik ; Lee, Kyung-Kwang</creatorcontrib><description>CAG trinucleotide repeat (CTR) sequence often appears in mammalian genome including transcription-regulatory protein and homeobox genes. Its expansion is associated with six genetic disorders in human. To identify novel CTR-containing genes expressed in mouse brain, a brain cDNA library was screened using an oligonucleotide, (CTG)10. Eight clones were novel mouse genes and they were sequenced on both strands. The size of the cloned DNA ranged from 0.5 to 2.1 kb. The number of the CAG repeats in the clones ranged from 6 to 25. The inserts of the clones were analyzed for open reading frames and the peptide sequences were used for a GenBank homology search. Of the clones, one (CAG-6) shared 13 consecutive identical amino acid residues with the OB-cadherin gene, a member of cadherin family. CAG-14 showed high homology (657 nucleotides identity in 1022 nucleotides; 64%) with the 3′-untranslated region of rat leukocyte common antigen-related (LAR) tyrosine phosphatase receptor. All the 8 clones were originated from mouse DNA as judged by Southern blot analysis of mouse genomic DNA. The expression of the clones in mouse brain was addressed by RT-PCR and 4 clones showed specific expression.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1997.7643</identifier><identifier>PMID: 9367917</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blotting, Southern ; Brain Chemistry - genetics ; Cloning, Molecular ; DNA, Complementary - isolation & purification ; Genes ; Genome ; Mice ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Trinucleotide Repeats</subject><ispartof>Biochemical and biophysical research communications, 1997-11, Vol.240 (1), p.239-243</ispartof><rights>1997 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-ed6bbd0eefc0f9345b702fe8e0097e63f4f6a14423635e3690f19f0fffe015ab3</citedby><cites>FETCH-LOGICAL-c339t-ed6bbd0eefc0f9345b702fe8e0097e63f4f6a14423635e3690f19f0fffe015ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X97976439$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9367917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Sun Jung</creatorcontrib><creatorcontrib>Shon, Bo Hwa</creatorcontrib><creatorcontrib>Kang, Joo Hyun</creatorcontrib><creatorcontrib>Hahm, Kyung-Soo</creatorcontrib><creatorcontrib>Yoo, Ook Joon</creatorcontrib><creatorcontrib>Park, Young Sik</creatorcontrib><creatorcontrib>Lee, Kyung-Kwang</creatorcontrib><title>Cloning of Novel Trinucleotide-Repeat (CAG) Containing Genes in Mouse Brain</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>CAG trinucleotide repeat (CTR) sequence often appears in mammalian genome including transcription-regulatory protein and homeobox genes. Its expansion is associated with six genetic disorders in human. To identify novel CTR-containing genes expressed in mouse brain, a brain cDNA library was screened using an oligonucleotide, (CTG)10. Eight clones were novel mouse genes and they were sequenced on both strands. The size of the cloned DNA ranged from 0.5 to 2.1 kb. The number of the CAG repeats in the clones ranged from 6 to 25. The inserts of the clones were analyzed for open reading frames and the peptide sequences were used for a GenBank homology search. Of the clones, one (CAG-6) shared 13 consecutive identical amino acid residues with the OB-cadherin gene, a member of cadherin family. CAG-14 showed high homology (657 nucleotides identity in 1022 nucleotides; 64%) with the 3′-untranslated region of rat leukocyte common antigen-related (LAR) tyrosine phosphatase receptor. All the 8 clones were originated from mouse DNA as judged by Southern blot analysis of mouse genomic DNA. The expression of the clones in mouse brain was addressed by RT-PCR and 4 clones showed specific expression.</description><subject>Animals</subject><subject>Blotting, Southern</subject><subject>Brain Chemistry - genetics</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Genes</subject><subject>Genome</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Trinucleotide Repeats</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kNFLwzAQh4Moc05ffRP6JPrQemmydHnUolOcCjLBt9CmF4l0zUzagf-9rRu--XRw992Pu4-QUwoJBRBXZel1QqXMkkxwtkfGFCTEKQW-T8bQE3Eq6fshOQrhE4BSLuSIjCQTmaTZmDzmtWts8xE5Ez27DdbR0tum0zW61lYYv-Iaiza6yK_nl1Humrawv_gcGwyRbaIn1wWMbnzfPyYHpqgDnuzqhLzd3S7z-3jxMn_IrxexZky2MVaiLCtANBqMZHxaZpAanCGAzFAww40oKOcpE2yKTEgwVBowxiDQaVGyCTnf5q69--owtGplg8a6Lhrsr1GZ5Gm_xXow2YLauxA8GrX2dlX4b0VBDfbUYE8N9tRgr1842yV35QqrP3ynq5_PtnPs39tY9Cpoi43GynrUraqc_S_6B9ohfZI</recordid><startdate>19971107</startdate><enddate>19971107</enddate><creator>Kim, Sun Jung</creator><creator>Shon, Bo Hwa</creator><creator>Kang, Joo Hyun</creator><creator>Hahm, Kyung-Soo</creator><creator>Yoo, Ook Joon</creator><creator>Park, Young Sik</creator><creator>Lee, Kyung-Kwang</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971107</creationdate><title>Cloning of Novel Trinucleotide-Repeat (CAG) Containing Genes in Mouse Brain</title><author>Kim, Sun Jung ; Shon, Bo Hwa ; Kang, Joo Hyun ; Hahm, Kyung-Soo ; Yoo, Ook Joon ; Park, Young Sik ; Lee, Kyung-Kwang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-ed6bbd0eefc0f9345b702fe8e0097e63f4f6a14423635e3690f19f0fffe015ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Blotting, Southern</topic><topic>Brain Chemistry - genetics</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Genes</topic><topic>Genome</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Polymerase Chain Reaction</topic><topic>Sequence Analysis, DNA</topic><topic>Trinucleotide Repeats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Sun Jung</creatorcontrib><creatorcontrib>Shon, Bo Hwa</creatorcontrib><creatorcontrib>Kang, Joo Hyun</creatorcontrib><creatorcontrib>Hahm, Kyung-Soo</creatorcontrib><creatorcontrib>Yoo, Ook Joon</creatorcontrib><creatorcontrib>Park, Young Sik</creatorcontrib><creatorcontrib>Lee, Kyung-Kwang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Sun Jung</au><au>Shon, Bo Hwa</au><au>Kang, Joo Hyun</au><au>Hahm, Kyung-Soo</au><au>Yoo, Ook Joon</au><au>Park, Young Sik</au><au>Lee, Kyung-Kwang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of Novel Trinucleotide-Repeat (CAG) Containing Genes in Mouse Brain</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1997-11-07</date><risdate>1997</risdate><volume>240</volume><issue>1</issue><spage>239</spage><epage>243</epage><pages>239-243</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>CAG trinucleotide repeat (CTR) sequence often appears in mammalian genome including transcription-regulatory protein and homeobox genes. Its expansion is associated with six genetic disorders in human. To identify novel CTR-containing genes expressed in mouse brain, a brain cDNA library was screened using an oligonucleotide, (CTG)10. Eight clones were novel mouse genes and they were sequenced on both strands. The size of the cloned DNA ranged from 0.5 to 2.1 kb. The number of the CAG repeats in the clones ranged from 6 to 25. The inserts of the clones were analyzed for open reading frames and the peptide sequences were used for a GenBank homology search. Of the clones, one (CAG-6) shared 13 consecutive identical amino acid residues with the OB-cadherin gene, a member of cadherin family. CAG-14 showed high homology (657 nucleotides identity in 1022 nucleotides; 64%) with the 3′-untranslated region of rat leukocyte common antigen-related (LAR) tyrosine phosphatase receptor. All the 8 clones were originated from mouse DNA as judged by Southern blot analysis of mouse genomic DNA. The expression of the clones in mouse brain was addressed by RT-PCR and 4 clones showed specific expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9367917</pmid><doi>10.1006/bbrc.1997.7643</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1997-11, Vol.240 (1), p.239-243 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79426903 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Blotting, Southern Brain Chemistry - genetics Cloning, Molecular DNA, Complementary - isolation & purification Genes Genome Mice Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction Sequence Analysis, DNA Trinucleotide Repeats |
| title | Cloning of Novel Trinucleotide-Repeat (CAG) Containing Genes in Mouse Brain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T03%3A33%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20of%20Novel%20Trinucleotide-Repeat%20(CAG)%20Containing%20Genes%20in%20Mouse%20Brain&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Kim,%20Sun%20Jung&rft.date=1997-11-07&rft.volume=240&rft.issue=1&rft.spage=239&rft.epage=243&rft.pages=239-243&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1006/bbrc.1997.7643&rft_dat=%3Cproquest_cross%3E79426903%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79426903&rft_id=info:pmid/9367917&rft_els_id=S0006291X97976439&rfr_iscdi=true |