Tyrosine Hydroxylase Phosphorylation in Digitonin‐Permeabilized Bovine Adrenal Chromaffin Cells: The Effect of Protein Kinase and Phosphatase Inhibitors on Ser19 and Ser40 Phosphorylation

: The protein kinases and protein phosphatases that act on tyrosine hydroxylase in vivo have not been established. Bovine adrenal chromaffin cells were permeabilized with digitonin and incubated with [γ‐32P]ATP, in the presence or absence of 10 µM Ca2+, 1 µM cyclic AMP, 1 µM phorbol dibutyrate, or v...

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