The mouse Plk gene: structural characterization, chromosomal localization and identification of a processed Plk pseudogene
The Plk gene encodes a serine/threonine protein kinase believed to be important for the normal progression of mammalian cells through the cell cycle. In this paper, we report the genomic organization of the mouse Plk gene. The mouse Plk gene encompasses 16 kb of the mouse genome and is organised int...
Gespeichert in:
| Veröffentlicht in: | Gene 1997-10, Vol.198 (1), p.329-339 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 339 |
|---|---|
| container_issue | 1 |
| container_start_page | 329 |
| container_title | Gene |
| container_volume | 198 |
| creator | Clay, Fiona J Ernst, Matthias R Trueman, John W.H Flegg, Robert Dunn, Ashley R |
| description | The
Plk gene encodes a serine/threonine protein kinase believed to be important for the normal progression of mammalian cells through the cell cycle. In this paper, we report the genomic organization of the mouse
Plk gene. The mouse
Plk gene encompasses 16
kb of the mouse genome and is organised into 10 exons. Based on homology with the human
PLK1 promoter region, the putative mouse promoter region includes a CCAAT motif but lacks the conventional TATA motif. The proposed promoter region contains consensus binding sites for several transcriptional regulators, including Sp1 and AP2. In addition to the active copy of
Plk, Plk exists as a processed pseudogene. Using RFLP analysis, we have localized the active
Plk gene to mouse Chromosome 7 and the processed pseudogene to mouse Chromosome 5. Southern blot analysis of DNA from a limited number of other mammalian species suggests that the duplication is confined to the mouse. Parsimony analysis suggests that the gene duplication leading to the mouse
Plk pseudogene occurred after the rat–mouse split. |
| doi_str_mv | 10.1016/S0378-1119(97)00335-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79418626</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378111997003351</els_id><sourcerecordid>16216195</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-fee7e99095621623f209154a46b5c8b107d4cb64523513b1a97f21a51fe063323</originalsourceid><addsrcrecordid>eNqFUU1P3DAQtRDVdgv8BCSfKpCa4rHjOOZSVagfSEhUYjlbjjMBt0m82AkS_Pp6P8SVuVgz78288TxCToF9BQbVxR0Tqi4AQJ9pdc6YELKAA7KEWukip_UhWb5RPpJPKf1lOaTkC7LQQjGu9ZK8rh6RDmFOSP_0_-gDjnhJ0xRnN83R9tQ92mjdhNG_2smH8UuuxDCEFIaM9sHZfo9QO7bUtzhOvvNuVwodtXQdg8OUsN0qrBPObdjoHJMPne0TnuzfI3L_88fq6ndxc_vr-ur7TeGEhqnoEBVqzbSsOFRcdJxpkKUtq0a6ugGm2tI1VSm5kCAasFp1HKyEDlklBBdH5PNubl7kacY0mcEnh31vR8wfN0qXUFe8epcImwVAy0yUO6KLIaWInVlHP9j4YoCZjTlma47ZXN5oZbbmGMh9p3uBuRmwfevau5Hxbzsc8zmePUaTnMfRYesjusm0wb-j8B_VEJ-Z</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16216195</pqid></control><display><type>article</type><title>The mouse Plk gene: structural characterization, chromosomal localization and identification of a processed Plk pseudogene</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Clay, Fiona J ; Ernst, Matthias R ; Trueman, John W.H ; Flegg, Robert ; Dunn, Ashley R</creator><creatorcontrib>Clay, Fiona J ; Ernst, Matthias R ; Trueman, John W.H ; Flegg, Robert ; Dunn, Ashley R</creatorcontrib><description>The
Plk gene encodes a serine/threonine protein kinase believed to be important for the normal progression of mammalian cells through the cell cycle. In this paper, we report the genomic organization of the mouse
Plk gene. The mouse
Plk gene encompasses 16
kb of the mouse genome and is organised into 10 exons. Based on homology with the human
PLK1 promoter region, the putative mouse promoter region includes a CCAAT motif but lacks the conventional TATA motif. The proposed promoter region contains consensus binding sites for several transcriptional regulators, including Sp1 and AP2. In addition to the active copy of
Plk, Plk exists as a processed pseudogene. Using RFLP analysis, we have localized the active
Plk gene to mouse Chromosome 7 and the processed pseudogene to mouse Chromosome 5. Southern blot analysis of DNA from a limited number of other mammalian species suggests that the duplication is confined to the mouse. Parsimony analysis suggests that the gene duplication leading to the mouse
Plk pseudogene occurred after the rat–mouse split.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(97)00335-1</identifier><identifier>PMID: 9370299</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Binding Sites ; Cell Cycle Proteins ; Chromosome Mapping ; Exons ; Gene structure, Parsimony analysis ; Genes ; Humans ; Introns ; Mice ; Molecular Sequence Data ; Multigene Family ; Phylogeny ; polo subfamily ; Polo-Like Kinase 1 ; Promoter Regions, Genetic ; Protein Kinases - genetics ; Protein Serine-Threonine Kinases ; Protein serine/threonine kinase ; Proto-Oncogene Proteins ; Pseudogenes ; Rats ; Sequence Homology, Nucleic Acid</subject><ispartof>Gene, 1997-10, Vol.198 (1), p.329-339</ispartof><rights>1997 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-fee7e99095621623f209154a46b5c8b107d4cb64523513b1a97f21a51fe063323</citedby><cites>FETCH-LOGICAL-c391t-fee7e99095621623f209154a46b5c8b107d4cb64523513b1a97f21a51fe063323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111997003351$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9370299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clay, Fiona J</creatorcontrib><creatorcontrib>Ernst, Matthias R</creatorcontrib><creatorcontrib>Trueman, John W.H</creatorcontrib><creatorcontrib>Flegg, Robert</creatorcontrib><creatorcontrib>Dunn, Ashley R</creatorcontrib><title>The mouse Plk gene: structural characterization, chromosomal localization and identification of a processed Plk pseudogene</title><title>Gene</title><addtitle>Gene</addtitle><description>The
Plk gene encodes a serine/threonine protein kinase believed to be important for the normal progression of mammalian cells through the cell cycle. In this paper, we report the genomic organization of the mouse
Plk gene. The mouse
Plk gene encompasses 16
kb of the mouse genome and is organised into 10 exons. Based on homology with the human
PLK1 promoter region, the putative mouse promoter region includes a CCAAT motif but lacks the conventional TATA motif. The proposed promoter region contains consensus binding sites for several transcriptional regulators, including Sp1 and AP2. In addition to the active copy of
Plk, Plk exists as a processed pseudogene. Using RFLP analysis, we have localized the active
Plk gene to mouse Chromosome 7 and the processed pseudogene to mouse Chromosome 5. Southern blot analysis of DNA from a limited number of other mammalian species suggests that the duplication is confined to the mouse. Parsimony analysis suggests that the gene duplication leading to the mouse
Plk pseudogene occurred after the rat–mouse split.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Cell Cycle Proteins</subject><subject>Chromosome Mapping</subject><subject>Exons</subject><subject>Gene structure, Parsimony analysis</subject><subject>Genes</subject><subject>Humans</subject><subject>Introns</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Phylogeny</subject><subject>polo subfamily</subject><subject>Polo-Like Kinase 1</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Kinases - genetics</subject><subject>Protein Serine-Threonine Kinases</subject><subject>Protein serine/threonine kinase</subject><subject>Proto-Oncogene Proteins</subject><subject>Pseudogenes</subject><subject>Rats</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1P3DAQtRDVdgv8BCSfKpCa4rHjOOZSVagfSEhUYjlbjjMBt0m82AkS_Pp6P8SVuVgz78288TxCToF9BQbVxR0Tqi4AQJ9pdc6YELKAA7KEWukip_UhWb5RPpJPKf1lOaTkC7LQQjGu9ZK8rh6RDmFOSP_0_-gDjnhJ0xRnN83R9tQ92mjdhNG_2smH8UuuxDCEFIaM9sHZfo9QO7bUtzhOvvNuVwodtXQdg8OUsN0qrBPObdjoHJMPne0TnuzfI3L_88fq6ndxc_vr-ur7TeGEhqnoEBVqzbSsOFRcdJxpkKUtq0a6ugGm2tI1VSm5kCAasFp1HKyEDlklBBdH5PNubl7kacY0mcEnh31vR8wfN0qXUFe8epcImwVAy0yUO6KLIaWInVlHP9j4YoCZjTlma47ZXN5oZbbmGMh9p3uBuRmwfevau5Hxbzsc8zmePUaTnMfRYesjusm0wb-j8B_VEJ-Z</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Clay, Fiona J</creator><creator>Ernst, Matthias R</creator><creator>Trueman, John W.H</creator><creator>Flegg, Robert</creator><creator>Dunn, Ashley R</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19971001</creationdate><title>The mouse Plk gene: structural characterization, chromosomal localization and identification of a processed Plk pseudogene</title><author>Clay, Fiona J ; Ernst, Matthias R ; Trueman, John W.H ; Flegg, Robert ; Dunn, Ashley R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-fee7e99095621623f209154a46b5c8b107d4cb64523513b1a97f21a51fe063323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Cell Cycle Proteins</topic><topic>Chromosome Mapping</topic><topic>Exons</topic><topic>Gene structure, Parsimony analysis</topic><topic>Genes</topic><topic>Humans</topic><topic>Introns</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Phylogeny</topic><topic>polo subfamily</topic><topic>Polo-Like Kinase 1</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Kinases - genetics</topic><topic>Protein Serine-Threonine Kinases</topic><topic>Protein serine/threonine kinase</topic><topic>Proto-Oncogene Proteins</topic><topic>Pseudogenes</topic><topic>Rats</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clay, Fiona J</creatorcontrib><creatorcontrib>Ernst, Matthias R</creatorcontrib><creatorcontrib>Trueman, John W.H</creatorcontrib><creatorcontrib>Flegg, Robert</creatorcontrib><creatorcontrib>Dunn, Ashley R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clay, Fiona J</au><au>Ernst, Matthias R</au><au>Trueman, John W.H</au><au>Flegg, Robert</au><au>Dunn, Ashley R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The mouse Plk gene: structural characterization, chromosomal localization and identification of a processed Plk pseudogene</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1997-10-01</date><risdate>1997</risdate><volume>198</volume><issue>1</issue><spage>329</spage><epage>339</epage><pages>329-339</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The
Plk gene encodes a serine/threonine protein kinase believed to be important for the normal progression of mammalian cells through the cell cycle. In this paper, we report the genomic organization of the mouse
Plk gene. The mouse
Plk gene encompasses 16
kb of the mouse genome and is organised into 10 exons. Based on homology with the human
PLK1 promoter region, the putative mouse promoter region includes a CCAAT motif but lacks the conventional TATA motif. The proposed promoter region contains consensus binding sites for several transcriptional regulators, including Sp1 and AP2. In addition to the active copy of
Plk, Plk exists as a processed pseudogene. Using RFLP analysis, we have localized the active
Plk gene to mouse Chromosome 7 and the processed pseudogene to mouse Chromosome 5. Southern blot analysis of DNA from a limited number of other mammalian species suggests that the duplication is confined to the mouse. Parsimony analysis suggests that the gene duplication leading to the mouse
Plk pseudogene occurred after the rat–mouse split.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9370299</pmid><doi>10.1016/S0378-1119(97)00335-1</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1997-10, Vol.198 (1), p.329-339 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79418626 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Base Sequence Binding Sites Cell Cycle Proteins Chromosome Mapping Exons Gene structure, Parsimony analysis Genes Humans Introns Mice Molecular Sequence Data Multigene Family Phylogeny polo subfamily Polo-Like Kinase 1 Promoter Regions, Genetic Protein Kinases - genetics Protein Serine-Threonine Kinases Protein serine/threonine kinase Proto-Oncogene Proteins Pseudogenes Rats Sequence Homology, Nucleic Acid |
| title | The mouse Plk gene: structural characterization, chromosomal localization and identification of a processed Plk pseudogene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T12%3A34%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20mouse%20Plk%20gene:%20structural%20characterization,%20chromosomal%20localization%20and%20identification%20of%20a%20processed%20Plk%20pseudogene&rft.jtitle=Gene&rft.au=Clay,%20Fiona%20J&rft.date=1997-10-01&rft.volume=198&rft.issue=1&rft.spage=329&rft.epage=339&rft.pages=329-339&rft.issn=0378-1119&rft.eissn=1879-0038&rft_id=info:doi/10.1016/S0378-1119(97)00335-1&rft_dat=%3Cproquest_cross%3E16216195%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16216195&rft_id=info:pmid/9370299&rft_els_id=S0378111997003351&rfr_iscdi=true |