Analysis of brain mRNA by reverse transcription-polymerase chain reaction and hybridization with digoxigenin-labeled DNA probe

The interleukin-1 family of polypeptides (IL-1 α, IL-1 β and IL-1 receptor antagonist (RA)) induces various centrally mediated host defense responses to infectious pathogens [3]. Considerable interest has focussed on IL-1 as a mediator in disease and in the production of systemic acute phase respons...

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Veröffentlicht in:	Brain research. Brain research protocols 1997-05, Vol.1 (2), p.145-151
Hauptverfasser:	Gabellec, Marie-Madeleine, Griffais, Rémy, Fillion, Gilles, Haour, France
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain - metabolism Central nervous system Digoxigenin DNA Probes Genome-octamer Interleukin-1 Interleukin-1 - genetics Interleukin-1 receptor Lipopolysaccharide Lipopolysaccharides - pharmacology Male Mice Mice, Inbred C3H Nucleic Acid Hybridization Polymerase Chain Reaction Primer Receptors, Interleukin-1 - antagonists & inhibitors Receptors, Interleukin-1 - genetics Reverse transcription-polymerase chain reaction RNA, Messenger - metabolism Transcription, Genetic
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