Granulocyte-macrophage colony-stimulating factor promotes development of in vitro produced bovine embryos
The objective was to determine whether granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates development of bovine embryos. In each experiment, oocytes were fertilized in vitro, GM-CSF was added to embryo culture medium at 8-10 h or 5 days after insemination, and development was monit...
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| Veröffentlicht in: | Biology of reproduction 1997-11, Vol.57 (5), p.1060-1065 |
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| container_title | Biology of reproduction |
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| creator | De Moraes, A.A.S. (University of Florida, Gainesville, FL.) Hansen, P.J |
| description | The objective was to determine whether granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates development of
bovine embryos. In each experiment, oocytes were fertilized in vitro, GM-CSF was added to embryo culture medium at 8-10 h
or 5 days after insemination, and development was monitored as the proportion of oocytes that formed blastocysts. Addition
of recombinant bovine GM-CSF to serum-free medium at 8-10 h after insemination increased the percentage of oocytes that formed
blastocysts (7.2% and 15.2% for control and GM-CSF, respectively). GM-CSF did not affect cleavage rate. Rather, the effect
of GM-CSF seems to be exerted after Day 5 after insemination, as indicated by the following findings: 1) GM-CSF did not alter
embryo cell number at Day 5 after insemination; 2) administration of GM-CSF at Day 5 increased the proportion of oocytes that
developed to the blastocyst stage (6.7%, 13.0%, and 22.4% for control and 1 and 10 ng/ml GM-CSF, respectively); and 3) addition
of serum at Day 5 increased development but prevented a further increase due to addition of GM-CSF at 10 h after insemination.
Blastocysts from GM-CSF-treated cultures tended to be at earlier stages of morphological development (i.e., fewer blastocysts
expanded at Day 7 and fewer were hatching or hatched at Day 9 after insemination). GM-CSF may play a role in the early development
of bovine embryos and might be a useful molecule for increasing blastocyst production rates in serum-free culture systems. |
| doi_str_mv | 10.1095/biolreprod57.5.1060 |
| format | Article |
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bovine embryos. In each experiment, oocytes were fertilized in vitro, GM-CSF was added to embryo culture medium at 8-10 h
or 5 days after insemination, and development was monitored as the proportion of oocytes that formed blastocysts. Addition
of recombinant bovine GM-CSF to serum-free medium at 8-10 h after insemination increased the percentage of oocytes that formed
blastocysts (7.2% and 15.2% for control and GM-CSF, respectively). GM-CSF did not affect cleavage rate. Rather, the effect
of GM-CSF seems to be exerted after Day 5 after insemination, as indicated by the following findings: 1) GM-CSF did not alter
embryo cell number at Day 5 after insemination; 2) administration of GM-CSF at Day 5 increased the proportion of oocytes that
developed to the blastocyst stage (6.7%, 13.0%, and 22.4% for control and 1 and 10 ng/ml GM-CSF, respectively); and 3) addition
of serum at Day 5 increased development but prevented a further increase due to addition of GM-CSF at 10 h after insemination.
Blastocysts from GM-CSF-treated cultures tended to be at earlier stages of morphological development (i.e., fewer blastocysts
expanded at Day 7 and fewer were hatching or hatched at Day 9 after insemination). GM-CSF may play a role in the early development
of bovine embryos and might be a useful molecule for increasing blastocyst production rates in serum-free culture systems.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod57.5.1060</identifier><identifier>PMID: 9369171</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; BLASTOCYST ; Blastocyst - drug effects ; Blastocyst - ultrastructure ; CATTLE ; Cell Count ; Cleavage Stage, Ovum - drug effects ; Culture Media, Serum-Free ; CYTOKINES ; Early stages. Segmentation. Gastrulation. Neurulation ; EMBRYO CULTURE ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development - drug effects ; EMBRYONIC DEVELOPMENT ; Female ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology ; Oocytes - drug effects ; Oocytes - growth & development ; Recombinant Proteins ; STIMULI</subject><ispartof>Biology of reproduction, 1997-11, Vol.57 (5), p.1060-1065</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2047733$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9369171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Moraes, A.A.S. (University of Florida, Gainesville, FL.)</creatorcontrib><creatorcontrib>Hansen, P.J</creatorcontrib><title>Granulocyte-macrophage colony-stimulating factor promotes development of in vitro produced bovine embryos</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The objective was to determine whether granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates development of
bovine embryos. In each experiment, oocytes were fertilized in vitro, GM-CSF was added to embryo culture medium at 8-10 h
or 5 days after insemination, and development was monitored as the proportion of oocytes that formed blastocysts. Addition
of recombinant bovine GM-CSF to serum-free medium at 8-10 h after insemination increased the percentage of oocytes that formed
blastocysts (7.2% and 15.2% for control and GM-CSF, respectively). GM-CSF did not affect cleavage rate. Rather, the effect
of GM-CSF seems to be exerted after Day 5 after insemination, as indicated by the following findings: 1) GM-CSF did not alter
embryo cell number at Day 5 after insemination; 2) administration of GM-CSF at Day 5 increased the proportion of oocytes that
developed to the blastocyst stage (6.7%, 13.0%, and 22.4% for control and 1 and 10 ng/ml GM-CSF, respectively); and 3) addition
of serum at Day 5 increased development but prevented a further increase due to addition of GM-CSF at 10 h after insemination.
Blastocysts from GM-CSF-treated cultures tended to be at earlier stages of morphological development (i.e., fewer blastocysts
expanded at Day 7 and fewer were hatching or hatched at Day 9 after insemination). GM-CSF may play a role in the early development
of bovine embryos and might be a useful molecule for increasing blastocyst production rates in serum-free culture systems.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BLASTOCYST</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - ultrastructure</subject><subject>CATTLE</subject><subject>Cell Count</subject><subject>Cleavage Stage, Ovum - drug effects</subject><subject>Culture Media, Serum-Free</subject><subject>CYTOKINES</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>EMBRYO CULTURE</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic and Fetal Development - drug effects</subject><subject>EMBRYONIC DEVELOPMENT</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - growth & development</subject><subject>Recombinant Proteins</subject><subject>STIMULI</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU9rGzEQxUVJSZ20n6AUdAi9raM_K2l1LCFNAoEc0pyFVjtrq0grV9La-Nt3TU1zmYF5P94bZhD6SsmaEi1ue59Chl1Og1Brscwk-YBWVDDdKCa7C7QihMiGc8k_oatSfhNCW874JbrUXGqq6Ar5h2ynOSR3rNBE63Labe0GsEshTcemVB_nYKufNni0rqaMl7yYKhQ8wB5C2kWYKk4j9hPe-5rTCRhmBwPu095PgCH2-ZjKZ_RxtKHAl3O_Rm8_73_dPTbPLw9Pdz-em5F1XT3VFiwIcJyI3oFwjlDR6c5y2QrBZWfbVqq-1wNnRLh-YJaOlDLHGJNE8mv0_Z_vssefGUo10RcHIdgJ0lyM0i1VgogF_HYG5z7CYHbZR5uP5nybRb8567Y4G8blUM6X_xgjrVKcv-dt_WZ78BlMiTaExZSbw-EglBHm9Jr3vNEmYzd58Xp7pVor0gmuGf8LfVqNfA</recordid><startdate>19971101</startdate><enddate>19971101</enddate><creator>De Moraes, A.A.S. (University of Florida, Gainesville, FL.)</creator><creator>Hansen, P.J</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19971101</creationdate><title>Granulocyte-macrophage colony-stimulating factor promotes development of in vitro produced bovine embryos</title><author>De Moraes, A.A.S. (University of Florida, Gainesville, FL.) ; Hansen, P.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f288t-f284eae5ec305bce5cc015898a36455368a4467bb9d3205cbd2a1f112c2226063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>BLASTOCYST</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - ultrastructure</topic><topic>CATTLE</topic><topic>Cell Count</topic><topic>Cleavage Stage, Ovum - drug effects</topic><topic>Culture Media, Serum-Free</topic><topic>CYTOKINES</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>EMBRYO CULTURE</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development - drug effects</topic><topic>EMBRYONIC DEVELOPMENT</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - growth & development</topic><topic>Recombinant Proteins</topic><topic>STIMULI</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Moraes, A.A.S. (University of Florida, Gainesville, FL.)</creatorcontrib><creatorcontrib>Hansen, P.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Moraes, A.A.S. (University of Florida, Gainesville, FL.)</au><au>Hansen, P.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Granulocyte-macrophage colony-stimulating factor promotes development of in vitro produced bovine embryos</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1997-11-01</date><risdate>1997</risdate><volume>57</volume><issue>5</issue><spage>1060</spage><epage>1065</epage><pages>1060-1065</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The objective was to determine whether granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates development of
bovine embryos. In each experiment, oocytes were fertilized in vitro, GM-CSF was added to embryo culture medium at 8-10 h
or 5 days after insemination, and development was monitored as the proportion of oocytes that formed blastocysts. Addition
of recombinant bovine GM-CSF to serum-free medium at 8-10 h after insemination increased the percentage of oocytes that formed
blastocysts (7.2% and 15.2% for control and GM-CSF, respectively). GM-CSF did not affect cleavage rate. Rather, the effect
of GM-CSF seems to be exerted after Day 5 after insemination, as indicated by the following findings: 1) GM-CSF did not alter
embryo cell number at Day 5 after insemination; 2) administration of GM-CSF at Day 5 increased the proportion of oocytes that
developed to the blastocyst stage (6.7%, 13.0%, and 22.4% for control and 1 and 10 ng/ml GM-CSF, respectively); and 3) addition
of serum at Day 5 increased development but prevented a further increase due to addition of GM-CSF at 10 h after insemination.
Blastocysts from GM-CSF-treated cultures tended to be at earlier stages of morphological development (i.e., fewer blastocysts
expanded at Day 7 and fewer were hatching or hatched at Day 9 after insemination). GM-CSF may play a role in the early development
of bovine embryos and might be a useful molecule for increasing blastocyst production rates in serum-free culture systems.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9369171</pmid><doi>10.1095/biolreprod57.5.1060</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1997-11, Vol.57 (5), p.1060-1065 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Biological and medical sciences BLASTOCYST Blastocyst - drug effects Blastocyst - ultrastructure CATTLE Cell Count Cleavage Stage, Ovum - drug effects Culture Media, Serum-Free CYTOKINES Early stages. Segmentation. Gastrulation. Neurulation EMBRYO CULTURE Embryology: invertebrates and vertebrates. Teratology Embryonic and Fetal Development - drug effects EMBRYONIC DEVELOPMENT Female Fertilization in Vitro Fundamental and applied biological sciences. Psychology Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Oocytes - drug effects Oocytes - growth & development Recombinant Proteins STIMULI |
| title | Granulocyte-macrophage colony-stimulating factor promotes development of in vitro produced bovine embryos |
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