Assessment of renal function by 51Cr-EDTA and endogenous creatinine clearances in the pig
In anaesthetized pigs, clearances of 51Cr‐EDTA (EDTA) and endogenous creatinine were compared with renal clearance of inulin measured during constant infusion after bolus injection. Creatinine was determined by enzymatic (Kodak Ektachem) as well as conventional (Jaffé) methods. In saline‐loaded pigs...
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| Veröffentlicht in: | Acta physiologica Scandinavica 1997-10, Vol.161 (2), p.253-260 |
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| creator | PALNAES HANSEN, C. BIE, P. STADIL, F. |
| description | In anaesthetized pigs, clearances of 51Cr‐EDTA (EDTA) and endogenous creatinine were compared with renal clearance of inulin measured during constant infusion after bolus injection. Creatinine was determined by enzymatic (Kodak Ektachem) as well as conventional (Jaffé) methods. In saline‐loaded pigs, renal clearance of constantly infused EDTA was 97.0 ± 6.7 mL min−1 and identical to the clearance of inulin (94.1 ± 9.1 mL min−1). There was good agreement between individual clearances. The extraction fractions of the two markers were indistinguishable (0.26 ± 0.02 and 0.28 ± 0.03, respectively). In other experiments the clearance of EDTA calculated from the first 4 h of the time course of the plasma concentration after single injection was 64.4 ± 3.7 mL min−1, correlating well with inulin clearance (63.0 ± 1.2 mL min−1). When calculated only from the monoexponential phase of the disappearance curve (`slope clearance'), significantly higher results were obtained (+33%, P |
| doi_str_mv | 10.1046/j.1365-201X.1997.00205.x |
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Creatinine was determined by enzymatic (Kodak Ektachem) as well as conventional (Jaffé) methods. In saline‐loaded pigs, renal clearance of constantly infused EDTA was 97.0 ± 6.7 mL min−1 and identical to the clearance of inulin (94.1 ± 9.1 mL min−1). There was good agreement between individual clearances. The extraction fractions of the two markers were indistinguishable (0.26 ± 0.02 and 0.28 ± 0.03, respectively). In other experiments the clearance of EDTA calculated from the first 4 h of the time course of the plasma concentration after single injection was 64.4 ± 3.7 mL min−1, correlating well with inulin clearance (63.0 ± 1.2 mL min−1). When calculated only from the monoexponential phase of the disappearance curve (`slope clearance'), significantly higher results were obtained (+33%, P < 0.001). Renal clearance of EDTA after single injection was 7.5 ± 1.5 mL min−1 (∼12%) lower than inulin clearance (P < 0.001). Values of creatinine clearances determined by the two analytical methods showed a poor agreement with inulin clearance. It is concluded that, in pigs, glomerular filtration rate may be estimated by the clearance of EDTA using constant infusion or single injection of EDTA and that the renal clearance of endogenous creatinine is a less useful a measure of GFR.</description><identifier>ISSN: 0001-6772</identifier><identifier>EISSN: 1365-201X</identifier><identifier>DOI: 10.1046/j.1365-201X.1997.00205.x</identifier><identifier>PMID: 9366968</identifier><identifier>CODEN: APSCAX</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Biological and medical sciences ; Chelating Agents - pharmacokinetics ; chrome edetic acid ; Chromium Radioisotopes ; Creatinine - blood ; Creatinine - metabolism ; Creatinine - urine ; Edetic Acid - pharmacokinetics ; Female ; Fundamental and applied biological sciences. Psychology ; glomerular filtration rate ; Glomerular Filtration Rate - physiology ; inulin clearance ; Jaffé ; Kidney Function Tests - methods ; Swine ; Vertebrates: urinary system</subject><ispartof>Acta physiologica Scandinavica, 1997-10, Vol.161 (2), p.253-260</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-201X.1997.00205.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-201X.1997.00205.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2838129$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9366968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PALNAES HANSEN, C.</creatorcontrib><creatorcontrib>BIE, P.</creatorcontrib><creatorcontrib>STADIL, F.</creatorcontrib><title>Assessment of renal function by 51Cr-EDTA and endogenous creatinine clearances in the pig</title><title>Acta physiologica Scandinavica</title><addtitle>Acta Physiol Scand</addtitle><description>In anaesthetized pigs, clearances of 51Cr‐EDTA (EDTA) and endogenous creatinine were compared with renal clearance of inulin measured during constant infusion after bolus injection. Creatinine was determined by enzymatic (Kodak Ektachem) as well as conventional (Jaffé) methods. In saline‐loaded pigs, renal clearance of constantly infused EDTA was 97.0 ± 6.7 mL min−1 and identical to the clearance of inulin (94.1 ± 9.1 mL min−1). There was good agreement between individual clearances. The extraction fractions of the two markers were indistinguishable (0.26 ± 0.02 and 0.28 ± 0.03, respectively). In other experiments the clearance of EDTA calculated from the first 4 h of the time course of the plasma concentration after single injection was 64.4 ± 3.7 mL min−1, correlating well with inulin clearance (63.0 ± 1.2 mL min−1). When calculated only from the monoexponential phase of the disappearance curve (`slope clearance'), significantly higher results were obtained (+33%, P < 0.001). Renal clearance of EDTA after single injection was 7.5 ± 1.5 mL min−1 (∼12%) lower than inulin clearance (P < 0.001). Values of creatinine clearances determined by the two analytical methods showed a poor agreement with inulin clearance. It is concluded that, in pigs, glomerular filtration rate may be estimated by the clearance of EDTA using constant infusion or single injection of EDTA and that the renal clearance of endogenous creatinine is a less useful a measure of GFR.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chelating Agents - pharmacokinetics</subject><subject>chrome edetic acid</subject><subject>Chromium Radioisotopes</subject><subject>Creatinine - blood</subject><subject>Creatinine - metabolism</subject><subject>Creatinine - urine</subject><subject>Edetic Acid - pharmacokinetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glomerular filtration rate</subject><subject>Glomerular Filtration Rate - physiology</subject><subject>inulin clearance</subject><subject>Jaffé</subject><subject>Kidney Function Tests - methods</subject><subject>Swine</subject><subject>Vertebrates: urinary system</subject><issn>0001-6772</issn><issn>1365-201X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kV1v0zAUhi0EGmXwE5B8gbhL8Ef8dYMUdWuHmGDShhjcWG58PFxSp8SpaP89Ca165WO9j14d-0EIU1JSUskP65JyKQpG6GNJjVElIYyIcv8Mzc7BczQjhNBCKsVeolc5r8cr14xdoAvDpTRSz9CPOmfIeQNpwF3APSTX4rBLzRC7hFcHLOi8L66vHmrskseQfPcEqdtl3PTghphiAty04HqXGsg4Jjz8AryNT6_Ri-DaDG9O5yX6trh-mN8Ut1-Xn-b1bRGpEosihCpIqiUxHqih0ngtpAgBBDdeqsCUd1qwFQW6Ys4z6ioaHCehYjIE7fklen_s3fbdnx3kwW5ibqBtXYJxT6tMRaXQ1Qi-PYG71Qa83fZx4_qDPf3FmL875S43rg3Ti2I-Y0xzTZkZsY9H7G9s4XCOKbGTGbu2kwA7CbCTGfvfjN3b-u6mHqfFWFAcC2IeYH8ucP1vKxVXwn7_srQ_7xef1ePy3t7xf7uKkNc</recordid><startdate>199710</startdate><enddate>199710</enddate><creator>PALNAES HANSEN, C.</creator><creator>BIE, P.</creator><creator>STADIL, F.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199710</creationdate><title>Assessment of renal function by 51Cr-EDTA and endogenous creatinine clearances in the pig</title><author>PALNAES HANSEN, C. ; BIE, P. ; STADIL, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i175F-ff4f618609de19169d8565ffe539d67f27da852b1e1b2ad21a41fa30f426ff8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chelating Agents - pharmacokinetics</topic><topic>chrome edetic acid</topic><topic>Chromium Radioisotopes</topic><topic>Creatinine - blood</topic><topic>Creatinine - metabolism</topic><topic>Creatinine - urine</topic><topic>Edetic Acid - pharmacokinetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glomerular filtration rate</topic><topic>Glomerular Filtration Rate - physiology</topic><topic>inulin clearance</topic><topic>Jaffé</topic><topic>Kidney Function Tests - methods</topic><topic>Swine</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PALNAES HANSEN, C.</creatorcontrib><creatorcontrib>BIE, P.</creatorcontrib><creatorcontrib>STADIL, F.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Acta physiologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PALNAES HANSEN, C.</au><au>BIE, P.</au><au>STADIL, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of renal function by 51Cr-EDTA and endogenous creatinine clearances in the pig</atitle><jtitle>Acta physiologica Scandinavica</jtitle><addtitle>Acta Physiol Scand</addtitle><date>1997-10</date><risdate>1997</risdate><volume>161</volume><issue>2</issue><spage>253</spage><epage>260</epage><pages>253-260</pages><issn>0001-6772</issn><eissn>1365-201X</eissn><coden>APSCAX</coden><abstract>In anaesthetized pigs, clearances of 51Cr‐EDTA (EDTA) and endogenous creatinine were compared with renal clearance of inulin measured during constant infusion after bolus injection. Creatinine was determined by enzymatic (Kodak Ektachem) as well as conventional (Jaffé) methods. In saline‐loaded pigs, renal clearance of constantly infused EDTA was 97.0 ± 6.7 mL min−1 and identical to the clearance of inulin (94.1 ± 9.1 mL min−1). There was good agreement between individual clearances. The extraction fractions of the two markers were indistinguishable (0.26 ± 0.02 and 0.28 ± 0.03, respectively). In other experiments the clearance of EDTA calculated from the first 4 h of the time course of the plasma concentration after single injection was 64.4 ± 3.7 mL min−1, correlating well with inulin clearance (63.0 ± 1.2 mL min−1). When calculated only from the monoexponential phase of the disappearance curve (`slope clearance'), significantly higher results were obtained (+33%, P < 0.001). Renal clearance of EDTA after single injection was 7.5 ± 1.5 mL min−1 (∼12%) lower than inulin clearance (P < 0.001). Values of creatinine clearances determined by the two analytical methods showed a poor agreement with inulin clearance. It is concluded that, in pigs, glomerular filtration rate may be estimated by the clearance of EDTA using constant infusion or single injection of EDTA and that the renal clearance of endogenous creatinine is a less useful a measure of GFR.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9366968</pmid><doi>10.1046/j.1365-201X.1997.00205.x</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological and medical sciences Chelating Agents - pharmacokinetics chrome edetic acid Chromium Radioisotopes Creatinine - blood Creatinine - metabolism Creatinine - urine Edetic Acid - pharmacokinetics Female Fundamental and applied biological sciences. Psychology glomerular filtration rate Glomerular Filtration Rate - physiology inulin clearance Jaffé Kidney Function Tests - methods Swine Vertebrates: urinary system |
| title | Assessment of renal function by 51Cr-EDTA and endogenous creatinine clearances in the pig |
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