Intraocular PMMA lenses modified with surface-immobilized heparin: evaluation of biocompatibility in vitro and in vivo
Intraocular lenses (IOL) were surface modified with covalently linked heparin. The surface-bound heparin could not be removed by incubation in solutions known to be effective in breaking non-covalent bonds, nor by incubation in a solution of proteinase K and only to a limited extent by incubation wi...
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| Veröffentlicht in: | Biomaterials 1989-10, Vol.10 (8), p.511-516 |
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| creator | Larsson, R. Selén, G. Björklund, H. Fagerholm, P. |
| description | Intraocular lenses (IOL) were surface modified with covalently linked heparin. The surface-bound heparin could not be removed by incubation in solutions known to be effective in breaking non-covalent bonds, nor by incubation in a solution of proteinase K and only to a limited extent by incubation with heparinase.
In vitro studies demonstrated improved biocompatibility by the heparin surface-modified lens with respect to outgrowth of fibroblasts and macrophages, activation of granulocytes and adhesion of platelets. These results were subsequently verified
in vivo in terms of less inflammatory cells on the lens surface and fewer incidences of synechiae after 3 and 6 wk IOL implantation in the rabbit eye. |
| doi_str_mv | 10.1016/0142-9612(89)90055-0 |
| format | Article |
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In vitro studies demonstrated improved biocompatibility by the heparin surface-modified lens with respect to outgrowth of fibroblasts and macrophages, activation of granulocytes and adhesion of platelets. These results were subsequently verified
in vivo in terms of less inflammatory cells on the lens surface and fewer incidences of synechiae after 3 and 6 wk IOL implantation in the rabbit eye.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/0142-9612(89)90055-0</identifier><identifier>PMID: 2605284</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Biocompatibility ; Biocompatible Materials ; Cell Adhesion - drug effects ; Fibroblasts - drug effects ; Granulocytes - drug effects ; heparin ; Heparin - pharmacology ; Humans ; In Vitro Techniques ; intraocular lenses ; Lenses, Intraocular ; Macrophages - drug effects ; Methylmethacrylates ; Platelet Adhesiveness - drug effects ; PMMA ; Prosthesis Design ; Rabbits ; Surface Properties</subject><ispartof>Biomaterials, 1989-10, Vol.10 (8), p.511-516</ispartof><rights>1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-79bff76cbb362768388a43638baeb72f8189a16b432a6d81acc4a4b9ba7333723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0142-9612(89)90055-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2605284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Larsson, R.</creatorcontrib><creatorcontrib>Selén, G.</creatorcontrib><creatorcontrib>Björklund, H.</creatorcontrib><creatorcontrib>Fagerholm, P.</creatorcontrib><title>Intraocular PMMA lenses modified with surface-immobilized heparin: evaluation of biocompatibility in vitro and in vivo</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Intraocular lenses (IOL) were surface modified with covalently linked heparin. The surface-bound heparin could not be removed by incubation in solutions known to be effective in breaking non-covalent bonds, nor by incubation in a solution of proteinase K and only to a limited extent by incubation with heparinase.
In vitro studies demonstrated improved biocompatibility by the heparin surface-modified lens with respect to outgrowth of fibroblasts and macrophages, activation of granulocytes and adhesion of platelets. These results were subsequently verified
in vivo in terms of less inflammatory cells on the lens surface and fewer incidences of synechiae after 3 and 6 wk IOL implantation in the rabbit eye.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials</subject><subject>Cell Adhesion - drug effects</subject><subject>Fibroblasts - drug effects</subject><subject>Granulocytes - drug effects</subject><subject>heparin</subject><subject>Heparin - pharmacology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>intraocular lenses</subject><subject>Lenses, Intraocular</subject><subject>Macrophages - drug effects</subject><subject>Methylmethacrylates</subject><subject>Platelet Adhesiveness - drug effects</subject><subject>PMMA</subject><subject>Prosthesis Design</subject><subject>Rabbits</subject><subject>Surface Properties</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtP3DAUha2qiA4D_6CVvKpgEfArfnSBhBBtkUB0AWvLdm6EqySe2kkQ_PpmOiOWXV0dnXPP1f0Q-kzJOSVUXhAqWGUkZafanBlC6roiH9CKaqWr2pD6I1q9Rz6ho1J-k0UTwQ7RIZOkZlqs0Hw7jNmlMHUu41_391e4g6FAwX1qYhuhwS9xfMZlyq0LUMW-Tz528W0xnmHjchy-YZhdN7kxpgGnFvuYQuo3i94Gx1ccBzzHMSfshmYn5nSMDlrXFTjZzzV6-n7zeP2zunv4cXt9dVcFpthYKePbVsngPZdMSc21doJLrr0Dr1irqTaOSi84c7LR1IUgnPDGO8U5V4yv0ddd7yanPxOU0faxBOg6N0CailVGUKaXxjUSu2DIqZQMrd3k2Lv8aimxW9x2y9JuWVpt7D_clixrX_b9k--heV_a8138y50Py5NzhGxLiDAEaGKGMNomxf8f-At_T5Bh</recordid><startdate>198910</startdate><enddate>198910</enddate><creator>Larsson, R.</creator><creator>Selén, G.</creator><creator>Björklund, H.</creator><creator>Fagerholm, P.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198910</creationdate><title>Intraocular PMMA lenses modified with surface-immobilized heparin: evaluation of biocompatibility in vitro and in vivo</title><author>Larsson, R. ; Selén, G. ; Björklund, H. ; Fagerholm, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-79bff76cbb362768388a43638baeb72f8189a16b432a6d81acc4a4b9ba7333723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials</topic><topic>Cell Adhesion - drug effects</topic><topic>Fibroblasts - drug effects</topic><topic>Granulocytes - drug effects</topic><topic>heparin</topic><topic>Heparin - pharmacology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>intraocular lenses</topic><topic>Lenses, Intraocular</topic><topic>Macrophages - drug effects</topic><topic>Methylmethacrylates</topic><topic>Platelet Adhesiveness - drug effects</topic><topic>PMMA</topic><topic>Prosthesis Design</topic><topic>Rabbits</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Larsson, R.</creatorcontrib><creatorcontrib>Selén, G.</creatorcontrib><creatorcontrib>Björklund, H.</creatorcontrib><creatorcontrib>Fagerholm, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Larsson, R.</au><au>Selén, G.</au><au>Björklund, H.</au><au>Fagerholm, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intraocular PMMA lenses modified with surface-immobilized heparin: evaluation of biocompatibility in vitro and in vivo</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>1989-10</date><risdate>1989</risdate><volume>10</volume><issue>8</issue><spage>511</spage><epage>516</epage><pages>511-516</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Intraocular lenses (IOL) were surface modified with covalently linked heparin. The surface-bound heparin could not be removed by incubation in solutions known to be effective in breaking non-covalent bonds, nor by incubation in a solution of proteinase K and only to a limited extent by incubation with heparinase.
In vitro studies demonstrated improved biocompatibility by the heparin surface-modified lens with respect to outgrowth of fibroblasts and macrophages, activation of granulocytes and adhesion of platelets. These results were subsequently verified
in vivo in terms of less inflammatory cells on the lens surface and fewer incidences of synechiae after 3 and 6 wk IOL implantation in the rabbit eye.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>2605284</pmid><doi>10.1016/0142-9612(89)90055-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 1989-10, Vol.10 (8), p.511-516 |
| issn | 0142-9612 1878-5905 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biocompatibility Biocompatible Materials Cell Adhesion - drug effects Fibroblasts - drug effects Granulocytes - drug effects heparin Heparin - pharmacology Humans In Vitro Techniques intraocular lenses Lenses, Intraocular Macrophages - drug effects Methylmethacrylates Platelet Adhesiveness - drug effects PMMA Prosthesis Design Rabbits Surface Properties |
| title | Intraocular PMMA lenses modified with surface-immobilized heparin: evaluation of biocompatibility in vitro and in vivo |
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