Convertase PC2 and the neuroendocrine polypeptide 7B2 are co-induced and processed during neuronal differentiation of P19 embryonal carcinoma cells
Convertases of the subtilisin/kexin family are responsible for the biological activation of a variety of pro-proteins, pro-hormones, and pro-trophic factors, and thus can modulate various aspects of embryonic development. We investigated the expression of each convertase by Northern hybridization du...
Gespeichert in:
| Veröffentlicht in: | DNA and cell biology 1997-10, Vol.16 (10), p.1175-1187 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1187 |
|---|---|
| container_issue | 10 |
| container_start_page | 1175 |
| container_title | DNA and cell biology |
| container_volume | 16 |
| creator | Jeannotte, R Paquin, J Petit-Turcotte, C Day, R |
| description | Convertases of the subtilisin/kexin family are responsible for the biological activation of a variety of pro-proteins, pro-hormones, and pro-trophic factors, and thus can modulate various aspects of embryonic development. We investigated the expression of each convertase by Northern hybridization during cell differentiation in vitro, using the mouse embryonal carcinoma cell line P19 as a model. The neuroendocrine convertase PC2 and 7B2, its specific binding protein, are co-induced during neuronal differentiation of P19 cells with retinoic acid, whereas the other convertases are not or follow different patterns of temporal expression. The mature forms of PC2 and 7B2 proteins are detected together by immunoblotting following induction of mRNA expression, indicating that these proteins are processed early during brain development. These results demonstrate that PC2 and 7B2 gene expression and protein processing are in a close temporal association during neuronal differentiation and point to the value of the P19 cell model to study the significance and the regulation of this relationship in mammalian brain development. |
| doi_str_mv | 10.1089/dna.1997.16.1175 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79410667</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79410667</sourcerecordid><originalsourceid>FETCH-LOGICAL-c325t-9803dd63c8ab0110fdb0abc5c65e93f7d475440aa50804f9b1fd1296ad14e25c3</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhq0KVErpnQuST9yyHcdf8RFWfFSqRA9wjhx70holdrATpP0d_GG83RVXTjMjPc-rkV5C3jLYMejMrY92x4zRO6Z2jGl5Qa6YlLrRgsOLuoMQjRSme0Vel_ITAGTL4JJcGq6Eabsr8mef4m_Mqy1IH_YttdHT9QlpxC0njD65HCLSJU2HBZc1eKT6Y8UyUpeaEP3m0D9bS04OS6mX36rzeIqIdqI-jCNmjGuwa0iRppE-MENxHvLhGXA2uxDTbKnDaSpvyMvRTgVvzvOa_Pj86fv-a3P_7cvd_sN943gr18Z0wL1X3HV2AMZg9APYwUmnJBo-ai-0FAKsldCBGM3ARs9ao6xnAlvp-DV5f8qtr__asKz9HMrxAxsxbaXXRjBQSv8XZEoI1fKugnACXU6lZBz7JYfZ5kPPoD8W1tfC-mNh1emPhVXl3Tl7G2b0_4RzQ_wv9LOTuw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16446238</pqid></control><display><type>article</type><title>Convertase PC2 and the neuroendocrine polypeptide 7B2 are co-induced and processed during neuronal differentiation of P19 embryonal carcinoma cells</title><source>Mary Ann Liebert Online Subscription</source><source>MEDLINE</source><creator>Jeannotte, R ; Paquin, J ; Petit-Turcotte, C ; Day, R</creator><creatorcontrib>Jeannotte, R ; Paquin, J ; Petit-Turcotte, C ; Day, R</creatorcontrib><description>Convertases of the subtilisin/kexin family are responsible for the biological activation of a variety of pro-proteins, pro-hormones, and pro-trophic factors, and thus can modulate various aspects of embryonic development. We investigated the expression of each convertase by Northern hybridization during cell differentiation in vitro, using the mouse embryonal carcinoma cell line P19 as a model. The neuroendocrine convertase PC2 and 7B2, its specific binding protein, are co-induced during neuronal differentiation of P19 cells with retinoic acid, whereas the other convertases are not or follow different patterns of temporal expression. The mature forms of PC2 and 7B2 proteins are detected together by immunoblotting following induction of mRNA expression, indicating that these proteins are processed early during brain development. These results demonstrate that PC2 and 7B2 gene expression and protein processing are in a close temporal association during neuronal differentiation and point to the value of the P19 cell model to study the significance and the regulation of this relationship in mammalian brain development.</description><identifier>ISSN: 1044-5498</identifier><identifier>EISSN: 1557-7430</identifier><identifier>DOI: 10.1089/dna.1997.16.1175</identifier><identifier>PMID: 9364928</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Carcinoma, Embryonal - enzymology ; Carcinoma, Embryonal - genetics ; Carcinoma, Embryonal - metabolism ; Cell Differentiation - drug effects ; Enzyme Induction ; Furin ; Gene Expression Regulation, Developmental - physiology ; Lewis X Antigen - analysis ; Mesoderm ; Mice ; Nerve Tissue Proteins - biosynthesis ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Neuroendocrine Secretory Protein 7B2 ; Neurofilament Proteins - analysis ; Neurons - cytology ; Neurons - enzymology ; Pituitary Hormones - biosynthesis ; Pituitary Hormones - genetics ; Pituitary Hormones - metabolism ; Proprotein Convertase 2 ; Proprotein Convertase 5 ; Proprotein Convertases ; Protein Processing, Post-Translational ; RNA, Messenger - analysis ; Serine Endopeptidases - genetics ; Subtilisins - biosynthesis ; Subtilisins - genetics ; Subtilisins - metabolism ; Tretinoin - pharmacology ; Tumor Cells, Cultured</subject><ispartof>DNA and cell biology, 1997-10, Vol.16 (10), p.1175-1187</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c325t-9803dd63c8ab0110fdb0abc5c65e93f7d475440aa50804f9b1fd1296ad14e25c3</citedby><cites>FETCH-LOGICAL-c325t-9803dd63c8ab0110fdb0abc5c65e93f7d475440aa50804f9b1fd1296ad14e25c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9364928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeannotte, R</creatorcontrib><creatorcontrib>Paquin, J</creatorcontrib><creatorcontrib>Petit-Turcotte, C</creatorcontrib><creatorcontrib>Day, R</creatorcontrib><title>Convertase PC2 and the neuroendocrine polypeptide 7B2 are co-induced and processed during neuronal differentiation of P19 embryonal carcinoma cells</title><title>DNA and cell biology</title><addtitle>DNA Cell Biol</addtitle><description>Convertases of the subtilisin/kexin family are responsible for the biological activation of a variety of pro-proteins, pro-hormones, and pro-trophic factors, and thus can modulate various aspects of embryonic development. We investigated the expression of each convertase by Northern hybridization during cell differentiation in vitro, using the mouse embryonal carcinoma cell line P19 as a model. The neuroendocrine convertase PC2 and 7B2, its specific binding protein, are co-induced during neuronal differentiation of P19 cells with retinoic acid, whereas the other convertases are not or follow different patterns of temporal expression. The mature forms of PC2 and 7B2 proteins are detected together by immunoblotting following induction of mRNA expression, indicating that these proteins are processed early during brain development. These results demonstrate that PC2 and 7B2 gene expression and protein processing are in a close temporal association during neuronal differentiation and point to the value of the P19 cell model to study the significance and the regulation of this relationship in mammalian brain development.</description><subject>Animals</subject><subject>Carcinoma, Embryonal - enzymology</subject><subject>Carcinoma, Embryonal - genetics</subject><subject>Carcinoma, Embryonal - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Enzyme Induction</subject><subject>Furin</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Lewis X Antigen - analysis</subject><subject>Mesoderm</subject><subject>Mice</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neuroendocrine Secretory Protein 7B2</subject><subject>Neurofilament Proteins - analysis</subject><subject>Neurons - cytology</subject><subject>Neurons - enzymology</subject><subject>Pituitary Hormones - biosynthesis</subject><subject>Pituitary Hormones - genetics</subject><subject>Pituitary Hormones - metabolism</subject><subject>Proprotein Convertase 2</subject><subject>Proprotein Convertase 5</subject><subject>Proprotein Convertases</subject><subject>Protein Processing, Post-Translational</subject><subject>RNA, Messenger - analysis</subject><subject>Serine Endopeptidases - genetics</subject><subject>Subtilisins - biosynthesis</subject><subject>Subtilisins - genetics</subject><subject>Subtilisins - metabolism</subject><subject>Tretinoin - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>1044-5498</issn><issn>1557-7430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhq0KVErpnQuST9yyHcdf8RFWfFSqRA9wjhx70holdrATpP0d_GG83RVXTjMjPc-rkV5C3jLYMejMrY92x4zRO6Z2jGl5Qa6YlLrRgsOLuoMQjRSme0Vel_ITAGTL4JJcGq6Eabsr8mef4m_Mqy1IH_YttdHT9QlpxC0njD65HCLSJU2HBZc1eKT6Y8UyUpeaEP3m0D9bS04OS6mX36rzeIqIdqI-jCNmjGuwa0iRppE-MENxHvLhGXA2uxDTbKnDaSpvyMvRTgVvzvOa_Pj86fv-a3P_7cvd_sN943gr18Z0wL1X3HV2AMZg9APYwUmnJBo-ai-0FAKsldCBGM3ARs9ao6xnAlvp-DV5f8qtr__asKz9HMrxAxsxbaXXRjBQSv8XZEoI1fKugnACXU6lZBz7JYfZ5kPPoD8W1tfC-mNh1emPhVXl3Tl7G2b0_4RzQ_wv9LOTuw</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Jeannotte, R</creator><creator>Paquin, J</creator><creator>Petit-Turcotte, C</creator><creator>Day, R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19971001</creationdate><title>Convertase PC2 and the neuroendocrine polypeptide 7B2 are co-induced and processed during neuronal differentiation of P19 embryonal carcinoma cells</title><author>Jeannotte, R ; Paquin, J ; Petit-Turcotte, C ; Day, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-9803dd63c8ab0110fdb0abc5c65e93f7d475440aa50804f9b1fd1296ad14e25c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Carcinoma, Embryonal - enzymology</topic><topic>Carcinoma, Embryonal - genetics</topic><topic>Carcinoma, Embryonal - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Enzyme Induction</topic><topic>Furin</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Lewis X Antigen - analysis</topic><topic>Mesoderm</topic><topic>Mice</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neuroendocrine Secretory Protein 7B2</topic><topic>Neurofilament Proteins - analysis</topic><topic>Neurons - cytology</topic><topic>Neurons - enzymology</topic><topic>Pituitary Hormones - biosynthesis</topic><topic>Pituitary Hormones - genetics</topic><topic>Pituitary Hormones - metabolism</topic><topic>Proprotein Convertase 2</topic><topic>Proprotein Convertase 5</topic><topic>Proprotein Convertases</topic><topic>Protein Processing, Post-Translational</topic><topic>RNA, Messenger - analysis</topic><topic>Serine Endopeptidases - genetics</topic><topic>Subtilisins - biosynthesis</topic><topic>Subtilisins - genetics</topic><topic>Subtilisins - metabolism</topic><topic>Tretinoin - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeannotte, R</creatorcontrib><creatorcontrib>Paquin, J</creatorcontrib><creatorcontrib>Petit-Turcotte, C</creatorcontrib><creatorcontrib>Day, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>DNA and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeannotte, R</au><au>Paquin, J</au><au>Petit-Turcotte, C</au><au>Day, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Convertase PC2 and the neuroendocrine polypeptide 7B2 are co-induced and processed during neuronal differentiation of P19 embryonal carcinoma cells</atitle><jtitle>DNA and cell biology</jtitle><addtitle>DNA Cell Biol</addtitle><date>1997-10-01</date><risdate>1997</risdate><volume>16</volume><issue>10</issue><spage>1175</spage><epage>1187</epage><pages>1175-1187</pages><issn>1044-5498</issn><eissn>1557-7430</eissn><abstract>Convertases of the subtilisin/kexin family are responsible for the biological activation of a variety of pro-proteins, pro-hormones, and pro-trophic factors, and thus can modulate various aspects of embryonic development. We investigated the expression of each convertase by Northern hybridization during cell differentiation in vitro, using the mouse embryonal carcinoma cell line P19 as a model. The neuroendocrine convertase PC2 and 7B2, its specific binding protein, are co-induced during neuronal differentiation of P19 cells with retinoic acid, whereas the other convertases are not or follow different patterns of temporal expression. The mature forms of PC2 and 7B2 proteins are detected together by immunoblotting following induction of mRNA expression, indicating that these proteins are processed early during brain development. These results demonstrate that PC2 and 7B2 gene expression and protein processing are in a close temporal association during neuronal differentiation and point to the value of the P19 cell model to study the significance and the regulation of this relationship in mammalian brain development.</abstract><cop>United States</cop><pmid>9364928</pmid><doi>10.1089/dna.1997.16.1175</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1044-5498 |
| ispartof | DNA and cell biology, 1997-10, Vol.16 (10), p.1175-1187 |
| issn | 1044-5498 1557-7430 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79410667 |
| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Animals Carcinoma, Embryonal - enzymology Carcinoma, Embryonal - genetics Carcinoma, Embryonal - metabolism Cell Differentiation - drug effects Enzyme Induction Furin Gene Expression Regulation, Developmental - physiology Lewis X Antigen - analysis Mesoderm Mice Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neuroendocrine Secretory Protein 7B2 Neurofilament Proteins - analysis Neurons - cytology Neurons - enzymology Pituitary Hormones - biosynthesis Pituitary Hormones - genetics Pituitary Hormones - metabolism Proprotein Convertase 2 Proprotein Convertase 5 Proprotein Convertases Protein Processing, Post-Translational RNA, Messenger - analysis Serine Endopeptidases - genetics Subtilisins - biosynthesis Subtilisins - genetics Subtilisins - metabolism Tretinoin - pharmacology Tumor Cells, Cultured |
| title | Convertase PC2 and the neuroendocrine polypeptide 7B2 are co-induced and processed during neuronal differentiation of P19 embryonal carcinoma cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T13%3A47%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Convertase%20PC2%20and%20the%20neuroendocrine%20polypeptide%207B2%20are%20co-induced%20and%20processed%20during%20neuronal%20differentiation%20of%20P19%20embryonal%20carcinoma%20cells&rft.jtitle=DNA%20and%20cell%20biology&rft.au=Jeannotte,%20R&rft.date=1997-10-01&rft.volume=16&rft.issue=10&rft.spage=1175&rft.epage=1187&rft.pages=1175-1187&rft.issn=1044-5498&rft.eissn=1557-7430&rft_id=info:doi/10.1089/dna.1997.16.1175&rft_dat=%3Cproquest_cross%3E79410667%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16446238&rft_id=info:pmid/9364928&rfr_iscdi=true |