Identification of a surrogate TSL‐1 antigen of Trichinella spiralis from a phage library of random peptides
We sought to find a peptide analogue of an important antigen (TSL‐1) of Trichinella spiralis which is recognized by the 7C2C5 antibody. A phage library which displays a short (15‐mer) randomly‐generated peptide at the filament of the minor coat protein of the virion was used for selection by the 7C2...
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| Veröffentlicht in: | Parasite immunology 1997-06, Vol.19 (6), p.281-286 |
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| container_end_page | 286 |
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| container_issue | 6 |
| container_start_page | 281 |
| container_title | Parasite immunology |
| container_volume | 19 |
| creator | LIM, PAK LEONG TAM, FRANKIE C.H. CHUI, YIU LOON NG, LAWRENCE W.K. |
| description | We sought to find a peptide analogue of an important antigen (TSL‐1) of Trichinella spiralis which is recognized by the 7C2C5 antibody. A phage library which displays a short (15‐mer) randomly‐generated peptide at the filament of the minor coat protein of the virion was used for selection by the 7C2C5 antibody. A peptide thus identified, ICDASGLGCWCWSLSP, was found to be a true surrogate since its binding to the antibody could be blocked by the native antigen and, conversely, an antiserum made to the peptide could recognize the native antigen. In addition, the peptide appeared to detect T. spiralis‐infected pigs although it was less discriminatory than the native antigen. |
| doi_str_mv | 10.1046/j.1365-3024.1997.d01-203.x |
| format | Article |
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A phage library which displays a short (15‐mer) randomly‐generated peptide at the filament of the minor coat protein of the virion was used for selection by the 7C2C5 antibody. A peptide thus identified, ICDASGLGCWCWSLSP, was found to be a true surrogate since its binding to the antibody could be blocked by the native antigen and, conversely, an antiserum made to the peptide could recognize the native antigen. In addition, the peptide appeared to detect T. spiralis‐infected pigs although it was less discriminatory than the native antigen.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9364558</pmid><doi>10.1046/j.1365-3024.1997.d01-203.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-9838 |
| ispartof | Parasite immunology, 1997-06, Vol.19 (6), p.281-286 |
| issn | 0141-9838 1365-3024 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79405572 |
| source | MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antigens, Helminth - analysis Antigens, Helminth - immunology Bacteriophages immunodiagnosis Mice Mice, Inbred BALB C Molecular Sequence Data peptide Peptide Library phage library Swine Trichinella spiralis Trichinella spiralis - immunology |
| title | Identification of a surrogate TSL‐1 antigen of Trichinella spiralis from a phage library of random peptides |
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