Measurement of serum levels of dehydroepiandrosterone sulfate: A comparison of radioimmunoassay and enzymatic analysis
Dehydroepiandrosterone sulfate (DHEAS) and unconjugated dehydroepiandrosterone (DHEA) are secretory products of the adrenal cortex. Measurement of serum levels of these steroids is of increasing epidemiologic interest, since low serum concentrations of DHEAS or DHEA have been associated with an incr...
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| Veröffentlicht in: | Steroids 1989-10, Vol.54 (4), p.355-371 |
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| creator | Holtzclaw, W.David Gordon, Gary B. |
| description | Dehydroepiandrosterone sulfate (DHEAS) and unconjugated dehydroepiandrosterone (DHEA) are secretory products of the adrenal cortex. Measurement of serum levels of these steroids is of increasing epidemiologic interest, since low serum concentrations of DHEAS or DHEA have been associated with an increased risk of dying of cardiovascular disease or of developing cancer. Radioimmunoassays (RIAs) are the most convenient systems for the measurement of serum DHEAS concentrations in multiple samples. However, using sera from four individuals we show that different RIA kits provide quite different estimates of serum DHEAS concentrations. Moreover, these results do not always agree with the serum concentrations determined by an independent Chromatographic and enzymatic reference method. The results highlight the need for an independent method of determining DHEAS levels in sera that can provide guidance in selecting an appropriate RIA, and in interpreting the results. |
| doi_str_mv | 10.1016/0039-128X(89)90050-0 |
| format | Article |
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Measurement of serum levels of these steroids is of increasing epidemiologic interest, since low serum concentrations of DHEAS or DHEA have been associated with an increased risk of dying of cardiovascular disease or of developing cancer. Radioimmunoassays (RIAs) are the most convenient systems for the measurement of serum DHEAS concentrations in multiple samples. However, using sera from four individuals we show that different RIA kits provide quite different estimates of serum DHEAS concentrations. Moreover, these results do not always agree with the serum concentrations determined by an independent Chromatographic and enzymatic reference method. The results highlight the need for an independent method of determining DHEAS levels in sera that can provide guidance in selecting an appropriate RIA, and in interpreting the results.</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/0039-128X(89)90050-0</identifier><identifier>PMID: 2532426</identifier><identifier>CODEN: STEDAM</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Dehydroepiandrosterone - analogs & derivatives ; Dehydroepiandrosterone - biosynthesis ; Dehydroepiandrosterone Sulfate ; Female ; Fluorometry ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoenzyme Techniques ; Male ; Middle Aged ; Radioimmunoassay ; Reagent Kits, Diagnostic ; Steroid hormones. 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Measurement of serum levels of these steroids is of increasing epidemiologic interest, since low serum concentrations of DHEAS or DHEA have been associated with an increased risk of dying of cardiovascular disease or of developing cancer. Radioimmunoassays (RIAs) are the most convenient systems for the measurement of serum DHEAS concentrations in multiple samples. However, using sera from four individuals we show that different RIA kits provide quite different estimates of serum DHEAS concentrations. Moreover, these results do not always agree with the serum concentrations determined by an independent Chromatographic and enzymatic reference method. The results highlight the need for an independent method of determining DHEAS levels in sera that can provide guidance in selecting an appropriate RIA, and in interpreting the results.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dehydroepiandrosterone - analogs & derivatives</subject><subject>Dehydroepiandrosterone - biosynthesis</subject><subject>Dehydroepiandrosterone Sulfate</subject><subject>Female</subject><subject>Fluorometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Radioimmunoassay</subject><subject>Reagent Kits, Diagnostic</subject><subject>Steroid hormones. Cholecalciferol derivatives</subject><subject>Vertebrates: endocrinology</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhhtR1nH1Hyj0RdFDayXpj2QPwrL4BSteFLyF6nQFI92dMdU90P56M86w3jwVRT1vUfUUxVMBrwWI9g2AMpWQ-vtLbV4ZgAYquFfshO501ei2u1_s7pCHxSPmnwDQKiMvigvZKFnLdlccPhPymmiieSmjL5nSOpUjHWjkYz_Qj21IkfYB51x5oRRnKnkdPS50VV6XLk57TIHjfOQTDiGGaVrniMy4lTlW0vx7m3AJLnc4bhz4cfHA48j05Fwvi2_v3329-Vjdfvnw6eb6tnIK5FLJum2V6wSQQuU9drIXxvd9D-j6ToHB_KfXrWta1Yu2UQpBklSkUXvQTl0WL0579yn-WokXOwV2NI44U1zZdqYGoRqRwfoEuvwkJ_J2n8KEabMC7FG3Pbq0R5dWG_tXt4Uce3bev_YTDXehs988f36eIzscfcLZBf6329QGaqEy9_bEZe10CJQsu0CzoyEkcosdYvj_IX8Ao9qeyw</recordid><startdate>198910</startdate><enddate>198910</enddate><creator>Holtzclaw, W.David</creator><creator>Gordon, Gary B.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198910</creationdate><title>Measurement of serum levels of dehydroepiandrosterone sulfate: A comparison of radioimmunoassay and enzymatic analysis</title><author>Holtzclaw, W.David ; Gordon, Gary B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-24663c710e3a3ffa72b19fbbb0acb7309a867f86c563b16533a02e23e8a8f08c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dehydroepiandrosterone - analogs & derivatives</topic><topic>Dehydroepiandrosterone - biosynthesis</topic><topic>Dehydroepiandrosterone Sulfate</topic><topic>Female</topic><topic>Fluorometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Radioimmunoassay</topic><topic>Reagent Kits, Diagnostic</topic><topic>Steroid hormones. Cholecalciferol derivatives</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holtzclaw, W.David</creatorcontrib><creatorcontrib>Gordon, Gary B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holtzclaw, W.David</au><au>Gordon, Gary B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of serum levels of dehydroepiandrosterone sulfate: A comparison of radioimmunoassay and enzymatic analysis</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>1989-10</date><risdate>1989</risdate><volume>54</volume><issue>4</issue><spage>355</spage><epage>371</epage><pages>355-371</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><coden>STEDAM</coden><abstract>Dehydroepiandrosterone sulfate (DHEAS) and unconjugated dehydroepiandrosterone (DHEA) are secretory products of the adrenal cortex. Measurement of serum levels of these steroids is of increasing epidemiologic interest, since low serum concentrations of DHEAS or DHEA have been associated with an increased risk of dying of cardiovascular disease or of developing cancer. Radioimmunoassays (RIAs) are the most convenient systems for the measurement of serum DHEAS concentrations in multiple samples. However, using sera from four individuals we show that different RIA kits provide quite different estimates of serum DHEAS concentrations. Moreover, these results do not always agree with the serum concentrations determined by an independent Chromatographic and enzymatic reference method. The results highlight the need for an independent method of determining DHEAS levels in sera that can provide guidance in selecting an appropriate RIA, and in interpreting the results.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2532426</pmid><doi>10.1016/0039-128X(89)90050-0</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0039-128X |
| ispartof | Steroids, 1989-10, Vol.54 (4), p.355-371 |
| issn | 0039-128X 1878-5867 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Adult Biological and medical sciences Chromatography, High Pressure Liquid Dehydroepiandrosterone - analogs & derivatives Dehydroepiandrosterone - biosynthesis Dehydroepiandrosterone Sulfate Female Fluorometry Fundamental and applied biological sciences. Psychology Humans Immunoenzyme Techniques Male Middle Aged Radioimmunoassay Reagent Kits, Diagnostic Steroid hormones. Cholecalciferol derivatives Vertebrates: endocrinology |
| title | Measurement of serum levels of dehydroepiandrosterone sulfate: A comparison of radioimmunoassay and enzymatic analysis |
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