Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis
Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, a...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 1997, Vol.59(10), pp.935-937 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Shimizu, A. (Kobe Univ. (Japan). Faculty of Agriculture) Kawano, J Yamamoto, C Kakutani, O Anzai, T Kamada, M |
| description | Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates. |
| doi_str_mv | 10.1292/jvms.59.935 |
| format | Article |
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(Kobe Univ. (Japan). Faculty of Agriculture) ; Kawano, J ; Yamamoto, C ; Kakutani, O ; Anzai, T ; Kamada, M</creator><creatorcontrib>Shimizu, A. (Kobe Univ. (Japan). Faculty of Agriculture) ; Kawano, J ; Yamamoto, C ; Kakutani, O ; Anzai, T ; Kamada, M</creatorcontrib><description>Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.59.935</identifier><identifier>PMID: 9362045</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; CABALLOS ; CHEVAL ; Dermatitis - microbiology ; Dermatitis - pathology ; Dermatitis - veterinary ; DNA FINGERPRINTING ; DNA, Bacterial - analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; ELECTROFORESIS ; ELECTROPHORESE ; ELECTROPHORESIS ; Electrophoresis, Gel, Pulsed-Field - methods ; Electrophoresis, Gel, Pulsed-Field - veterinary ; EMPREINTE ADN ; ENDOMETRITE ; ENDOMETRITIS ; Endometritis - microbiology ; Endometritis - pathology ; Endometritis - veterinary ; equine ; Female ; FENOTIPOS ; GENE ; GENERO HUMANO ; GENES ; GENOMAS ; GENOME ; GENOMES ; GENRE HUMAIN ; Horse Diseases - microbiology ; Horse Diseases - pathology ; HORSES ; HUELLAS GENETICAS ADN ; Humans ; Male ; MANKIND ; Methicillin Resistance - genetics ; methicillin-resistant ; PHENOTYPE ; PHENOTYPES ; pulsed-field gel electrophoresis ; Skin - microbiology ; Skin - pathology ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - pathology ; Staphylococcal Infections - veterinary ; STAPHYLOCOCCUS AUREUS ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Uterus - microbiology ; Uterus - pathology</subject><ispartof>Journal of Veterinary Medical Science, 1997, Vol.59(10), pp.935-937</ispartof><rights>1997 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c653t-77edcbb8421ddb99fcf1f7caa3407fab9a966722628411bb82649b65f21435693</citedby><cites>FETCH-LOGICAL-c653t-77edcbb8421ddb99fcf1f7caa3407fab9a966722628411bb82649b65f21435693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9362045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimizu, A. (Kobe Univ. (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Kawano, J</creatorcontrib><creatorcontrib>Yamamoto, C</creatorcontrib><creatorcontrib>Kakutani, O</creatorcontrib><creatorcontrib>Anzai, T</creatorcontrib><creatorcontrib>Kamada, M</creatorcontrib><title>Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates.</description><subject>Animals</subject><subject>CABALLOS</subject><subject>CHEVAL</subject><subject>Dermatitis - microbiology</subject><subject>Dermatitis - pathology</subject><subject>Dermatitis - veterinary</subject><subject>DNA FINGERPRINTING</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>ELECTROFORESIS</subject><subject>ELECTROPHORESE</subject><subject>ELECTROPHORESIS</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Electrophoresis, Gel, Pulsed-Field - veterinary</subject><subject>EMPREINTE ADN</subject><subject>ENDOMETRITE</subject><subject>ENDOMETRITIS</subject><subject>Endometritis - microbiology</subject><subject>Endometritis - pathology</subject><subject>Endometritis - veterinary</subject><subject>equine</subject><subject>Female</subject><subject>FENOTIPOS</subject><subject>GENE</subject><subject>GENERO HUMANO</subject><subject>GENES</subject><subject>GENOMAS</subject><subject>GENOME</subject><subject>GENOMES</subject><subject>GENRE HUMAIN</subject><subject>Horse Diseases - microbiology</subject><subject>Horse Diseases - pathology</subject><subject>HORSES</subject><subject>HUELLAS GENETICAS ADN</subject><subject>Humans</subject><subject>Male</subject><subject>MANKIND</subject><subject>Methicillin Resistance - genetics</subject><subject>methicillin-resistant</subject><subject>PHENOTYPE</subject><subject>PHENOTYPES</subject><subject>pulsed-field gel electrophoresis</subject><subject>Skin - microbiology</subject><subject>Skin - pathology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Infections - pathology</subject><subject>Staphylococcal Infections - veterinary</subject><subject>STAPHYLOCOCCUS AUREUS</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Uterus - microbiology</subject><subject>Uterus - pathology</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-L1DAYhoMo67h68iwUBC_SMb-aNEd30VVZUFDPIU2_zKSkTTdphfnvzTjDCF68JIf3yRO-70XoJcFbQhV9N_wa87ZRW8WaR2hDGJe15Ew9RhusiKglbfBT9CznAWNKuFBX6EoxQTFvNmi4gwkWbyszmXDIPlfRVfCw-gmqEZa9tz4EP9UJSraYaam-L2beH0K00do1V2ZNUK7uUM1ryNDXzkPoqx2ECgLYJcV5H_-8fo6eOFOQF-f7Gv38-OHH7af6_uvd59v397UVDVtqKaG3XddySvq-U8pZR5y0xjCOpTOdMkoISamgLSekgFRw1YnGldlYIxS7Rm9O3jnFhxXyokefLYRgJohr1lJxjFUr_gsSpZRk8mh8_Q84xDWVhRWGi1ZITvBR9_ZE2RRzTuD0nPxo0kETrI9F6WNRulG6FFXoV2fn2o3QX9hzMyW_OeVDWfsOLrlJpa0AF1dxn84ivYR2b5KG6e8nzkRtdsln_eVbGavFmIlGsd86WrD6</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Shimizu, A. (Kobe Univ. (Japan). Faculty of Agriculture)</creator><creator>Kawano, J</creator><creator>Yamamoto, C</creator><creator>Kakutani, O</creator><creator>Anzai, T</creator><creator>Kamada, M</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7QL</scope><scope>C1K</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1997</creationdate><title>Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis</title><author>Shimizu, A. (Kobe Univ. (Japan). Faculty of Agriculture) ; Kawano, J ; Yamamoto, C ; Kakutani, O ; Anzai, T ; Kamada, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c653t-77edcbb8421ddb99fcf1f7caa3407fab9a966722628411bb82649b65f21435693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>CABALLOS</topic><topic>CHEVAL</topic><topic>Dermatitis - microbiology</topic><topic>Dermatitis - pathology</topic><topic>Dermatitis - veterinary</topic><topic>DNA FINGERPRINTING</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>ELECTROFORESIS</topic><topic>ELECTROPHORESE</topic><topic>ELECTROPHORESIS</topic><topic>Electrophoresis, Gel, Pulsed-Field - methods</topic><topic>Electrophoresis, Gel, Pulsed-Field - veterinary</topic><topic>EMPREINTE ADN</topic><topic>ENDOMETRITE</topic><topic>ENDOMETRITIS</topic><topic>Endometritis - microbiology</topic><topic>Endometritis - pathology</topic><topic>Endometritis - veterinary</topic><topic>equine</topic><topic>Female</topic><topic>FENOTIPOS</topic><topic>GENE</topic><topic>GENERO HUMANO</topic><topic>GENES</topic><topic>GENOMAS</topic><topic>GENOME</topic><topic>GENOMES</topic><topic>GENRE HUMAIN</topic><topic>Horse Diseases - microbiology</topic><topic>Horse Diseases - pathology</topic><topic>HORSES</topic><topic>HUELLAS GENETICAS ADN</topic><topic>Humans</topic><topic>Male</topic><topic>MANKIND</topic><topic>Methicillin Resistance - genetics</topic><topic>methicillin-resistant</topic><topic>PHENOTYPE</topic><topic>PHENOTYPES</topic><topic>pulsed-field gel electrophoresis</topic><topic>Skin - microbiology</topic><topic>Skin - pathology</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Infections - pathology</topic><topic>Staphylococcal Infections - veterinary</topic><topic>STAPHYLOCOCCUS AUREUS</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Uterus - microbiology</topic><topic>Uterus - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimizu, A. 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Faculty of Agriculture)</creatorcontrib><creatorcontrib>Kawano, J</creatorcontrib><creatorcontrib>Yamamoto, C</creatorcontrib><creatorcontrib>Kakutani, O</creatorcontrib><creatorcontrib>Anzai, T</creatorcontrib><creatorcontrib>Kamada, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimizu, A. (Kobe Univ. (Japan). Faculty of Agriculture)</au><au>Kawano, J</au><au>Yamamoto, C</au><au>Kakutani, O</au><au>Anzai, T</au><au>Kamada, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>1997</date><risdate>1997</risdate><volume>59</volume><issue>10</issue><spage>935</spage><epage>937</epage><pages>935-937</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>9362045</pmid><doi>10.1292/jvms.59.935</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Veterinary Medical Science, 1997, Vol.59(10), pp.935-937 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_79400986 |
| source | J-STAGE (Free - Japanese); MEDLINE; Free E-Journal (出版社公開部分のみ）; Alma/SFX Local Collection |
| subjects | Animals CABALLOS CHEVAL Dermatitis - microbiology Dermatitis - pathology Dermatitis - veterinary DNA FINGERPRINTING DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics ELECTROFORESIS ELECTROPHORESE ELECTROPHORESIS Electrophoresis, Gel, Pulsed-Field - methods Electrophoresis, Gel, Pulsed-Field - veterinary EMPREINTE ADN ENDOMETRITE ENDOMETRITIS Endometritis - microbiology Endometritis - pathology Endometritis - veterinary equine Female FENOTIPOS GENE GENERO HUMANO GENES GENOMAS GENOME GENOMES GENRE HUMAIN Horse Diseases - microbiology Horse Diseases - pathology HORSES HUELLAS GENETICAS ADN Humans Male MANKIND Methicillin Resistance - genetics methicillin-resistant PHENOTYPE PHENOTYPES pulsed-field gel electrophoresis Skin - microbiology Skin - pathology Staphylococcal Infections - microbiology Staphylococcal Infections - pathology Staphylococcal Infections - veterinary STAPHYLOCOCCUS AUREUS Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Uterus - microbiology Uterus - pathology |
| title | Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis |
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