Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes
2-Mercaptoethanol (2-ME) is used as a medium supplement to enhance the proliferation of lymphocytes cultured in vitro. In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocyt...
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| Veröffentlicht in: | Cell biology and toxicology 1989-11, Vol.5 (3), p.237-248 |
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| container_title | Cell biology and toxicology |
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| creator | Aidoo, A Morris, S M Domon, O E McGarrity, L J Kodell, R L Casciano, D A |
| description | 2-Mercaptoethanol (2-ME) is used as a medium supplement to enhance the proliferation of lymphocytes cultured in vitro. In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocytes. There were virtually no metaphases detected in cells cultured without PHA. In PHA-stimulated cultures, 2-ME decreased SCE frequency but it enhanced SCE frequency in the presence of 5 to 12.5 microM bromodeoxyuridine (BRdU). Both mitotic and replication indices were increased in the PHA/2-ME system. The levels of incorporated exogenous thymidine, in the presence of 2-ME, were relatively low in unstimulated cells, suggesting that 2-ME is not mitogenic for T-cells. However, 2-ME enhanced PHA-induced response of T-cells as evidenced by increased levels of thymidine incorporation into cellular DNA. The growth-promoting effects and the decrease in SCE frequency caused by 2-ME upon PHA stimulation indicate that 2-ME may alter the nature of interaction between PHA and cellular activating properties or the replicative processes. |
| doi_str_mv | 10.1007/BF01795353 |
| format | Article |
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In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocytes. There were virtually no metaphases detected in cells cultured without PHA. In PHA-stimulated cultures, 2-ME decreased SCE frequency but it enhanced SCE frequency in the presence of 5 to 12.5 microM bromodeoxyuridine (BRdU). Both mitotic and replication indices were increased in the PHA/2-ME system. The levels of incorporated exogenous thymidine, in the presence of 2-ME, were relatively low in unstimulated cells, suggesting that 2-ME is not mitogenic for T-cells. However, 2-ME enhanced PHA-induced response of T-cells as evidenced by increased levels of thymidine incorporation into cellular DNA. The growth-promoting effects and the decrease in SCE frequency caused by 2-ME upon PHA stimulation indicate that 2-ME may alter the nature of interaction between PHA and cellular activating properties or the replicative processes.</description><identifier>ISSN: 0742-2091</identifier><identifier>EISSN: 1573-6822</identifier><identifier>DOI: 10.1007/BF01795353</identifier><identifier>PMID: 2598083</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Bromodeoxyuridine - pharmacology ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cells, Cultured ; Lymphocyte Activation - drug effects ; Lymphocytes - drug effects ; Lymphocytes - ultrastructure ; Male ; Mercaptoethanol - pharmacology ; Mitosis - drug effects ; Phytohemagglutinins - pharmacology ; Rats ; Rats, Inbred F344 ; Sister Chromatid Exchange - drug effects ; Thymidine - metabolism</subject><ispartof>Cell biology and toxicology, 1989-11, Vol.5 (3), p.237-248</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-b6e40fdcf99cec81675aa05e2115cad41bceb125a669826c649db97dfd4ffff93</citedby><cites>FETCH-LOGICAL-c282t-b6e40fdcf99cec81675aa05e2115cad41bceb125a669826c649db97dfd4ffff93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2598083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aidoo, A</creatorcontrib><creatorcontrib>Morris, S M</creatorcontrib><creatorcontrib>Domon, O E</creatorcontrib><creatorcontrib>McGarrity, L J</creatorcontrib><creatorcontrib>Kodell, R L</creatorcontrib><creatorcontrib>Casciano, D A</creatorcontrib><title>Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes</title><title>Cell biology and toxicology</title><addtitle>Cell Biol Toxicol</addtitle><description>2-Mercaptoethanol (2-ME) is used as a medium supplement to enhance the proliferation of lymphocytes cultured in vitro. In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocytes. There were virtually no metaphases detected in cells cultured without PHA. In PHA-stimulated cultures, 2-ME decreased SCE frequency but it enhanced SCE frequency in the presence of 5 to 12.5 microM bromodeoxyuridine (BRdU). Both mitotic and replication indices were increased in the PHA/2-ME system. The levels of incorporated exogenous thymidine, in the presence of 2-ME, were relatively low in unstimulated cells, suggesting that 2-ME is not mitogenic for T-cells. However, 2-ME enhanced PHA-induced response of T-cells as evidenced by increased levels of thymidine incorporation into cellular DNA. The growth-promoting effects and the decrease in SCE frequency caused by 2-ME upon PHA stimulation indicate that 2-ME may alter the nature of interaction between PHA and cellular activating properties or the replicative processes.</description><subject>Animals</subject><subject>Bromodeoxyuridine - pharmacology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cells, Cultured</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - ultrastructure</subject><subject>Male</subject><subject>Mercaptoethanol - pharmacology</subject><subject>Mitosis - drug effects</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Sister Chromatid Exchange - drug effects</subject><subject>Thymidine - metabolism</subject><issn>0742-2091</issn><issn>1573-6822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LwzAcxYMoc04v3oWcPAjVJG2S5qhjU2HiQT2XND-2SNvUJgX739u6od_Lgy-f9-A9AC4xusUI8buHNcJc0JSmR2COKU8TlhNyDOaIZyQhSOBTcBbCJ0KIYU5nYEaoyFGezsH3i9d9JaPzDfQWvi1X0DW6V78P2WioTFXBtvOVs6bbc-UASVKbTsk2ehN3svHV6ILtboh-Z2q53VZ9dI1rkhBdPcUbDUczrIa63Xk1RBPOwYmVVTAXB12Aj_XqffmUbF4fn5f3m0SRnMSkZCZDVisrhDIqx4xTKRE1BGOqpM5wqUyJCZWMiZwwxTKhS8G11ZkdT6QLcL3PHTt89SbEonZhKiUb4_tQcJHmXOAJvNmDqvMhdMYWbedq2Q0FRsU0c_E_8whfHVL7sjb6Dz3smv4A8k17Kw</recordid><startdate>198911</startdate><enddate>198911</enddate><creator>Aidoo, A</creator><creator>Morris, S M</creator><creator>Domon, O E</creator><creator>McGarrity, L J</creator><creator>Kodell, R L</creator><creator>Casciano, D A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198911</creationdate><title>Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes</title><author>Aidoo, A ; Morris, S M ; Domon, O E ; McGarrity, L J ; Kodell, R L ; Casciano, D A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-b6e40fdcf99cec81675aa05e2115cad41bceb125a669826c649db97dfd4ffff93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Bromodeoxyuridine - pharmacology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cells, Cultured</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - ultrastructure</topic><topic>Male</topic><topic>Mercaptoethanol - pharmacology</topic><topic>Mitosis - drug effects</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Sister Chromatid Exchange - drug effects</topic><topic>Thymidine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aidoo, A</creatorcontrib><creatorcontrib>Morris, S M</creatorcontrib><creatorcontrib>Domon, O E</creatorcontrib><creatorcontrib>McGarrity, L J</creatorcontrib><creatorcontrib>Kodell, R L</creatorcontrib><creatorcontrib>Casciano, D A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology and toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aidoo, A</au><au>Morris, S M</au><au>Domon, O E</au><au>McGarrity, L J</au><au>Kodell, R L</au><au>Casciano, D A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes</atitle><jtitle>Cell biology and toxicology</jtitle><addtitle>Cell Biol Toxicol</addtitle><date>1989-11</date><risdate>1989</risdate><volume>5</volume><issue>3</issue><spage>237</spage><epage>248</epage><pages>237-248</pages><issn>0742-2091</issn><eissn>1573-6822</eissn><abstract>2-Mercaptoethanol (2-ME) is used as a medium supplement to enhance the proliferation of lymphocytes cultured in vitro. In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocytes. There were virtually no metaphases detected in cells cultured without PHA. In PHA-stimulated cultures, 2-ME decreased SCE frequency but it enhanced SCE frequency in the presence of 5 to 12.5 microM bromodeoxyuridine (BRdU). Both mitotic and replication indices were increased in the PHA/2-ME system. The levels of incorporated exogenous thymidine, in the presence of 2-ME, were relatively low in unstimulated cells, suggesting that 2-ME is not mitogenic for T-cells. However, 2-ME enhanced PHA-induced response of T-cells as evidenced by increased levels of thymidine incorporation into cellular DNA. The growth-promoting effects and the decrease in SCE frequency caused by 2-ME upon PHA stimulation indicate that 2-ME may alter the nature of interaction between PHA and cellular activating properties or the replicative processes.</abstract><cop>Netherlands</cop><pmid>2598083</pmid><doi>10.1007/BF01795353</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0742-2091 |
| ispartof | Cell biology and toxicology, 1989-11, Vol.5 (3), p.237-248 |
| issn | 0742-2091 1573-6822 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Bromodeoxyuridine - pharmacology Cell Differentiation - drug effects Cell Division - drug effects Cells, Cultured Lymphocyte Activation - drug effects Lymphocytes - drug effects Lymphocytes - ultrastructure Male Mercaptoethanol - pharmacology Mitosis - drug effects Phytohemagglutinins - pharmacology Rats Rats, Inbred F344 Sister Chromatid Exchange - drug effects Thymidine - metabolism |
| title | Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes |
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