Sequence and transcript analysis of the C. albicans URA3 gene encoding orotidine-5'-phosphate decarboxylase
The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to...
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| Veröffentlicht in: | Current genetics 1989-09, Vol.16 (3), p.153-157 |
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| container_title | Current genetics |
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| creator | LOSBERGER, C ERNST, J. F |
| description | The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to a protein of 270 amino acids that shows homology to orotidine-5'-phosphate decarboxylases (ODCases) of other fungal species. The C. albicans ODCase is most closely related to the ODCases of the budding yeasts Kluyveromyces lactis and S. cerevisiae (74% and 71% homology, respectively). Most 5' ends of URA3 transcripts in the authentic host and in the heterologous host S. cerevisiae were found to be identical. These results demonstrate a close taxonomic relationship between non-pathogenic budding yeasts and C. albicans. |
| doi_str_mv | 10.1007/bf00391471 |
| format | Article |
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F</creatorcontrib><title>Sequence and transcript analysis of the C. albicans URA3 gene encoding orotidine-5'-phosphate decarboxylase</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>The human pathogen Candida albicans grows either as a yeast or in filamentous form. We have determined the sequence of a 1.365 kb genomic C. albicans fragment that complements Saccharomyces cerevisiae ura3 and Escherichia coli pyrF mutations. An open reading frame within this fragment corresponds to a protein of 270 amino acids that shows homology to orotidine-5'-phosphate decarboxylases (ODCases) of other fungal species. The C. albicans ODCase is most closely related to the ODCases of the budding yeasts Kluyveromyces lactis and S. cerevisiae (74% and 71% homology, respectively). Most 5' ends of URA3 transcripts in the authentic host and in the heterologous host S. cerevisiae were found to be identical. These results demonstrate a close taxonomic relationship between non-pathogenic budding yeasts and C. albicans.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Candida albicans - enzymology</subject><subject>Candida albicans - genetics</subject><subject>Carboxy-Lyases - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. 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F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-dceaa5f66ac343be81498d21032886509b0402faa98168b00ec20d30e39508233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Candida albicans - enzymology</topic><topic>Candida albicans - genetics</topic><topic>Carboxy-Lyases - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Kluyveromyces - enzymology</topic><topic>Kluyveromyces - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Orotidine-5'-Phosphate Decarboxylase - genetics</topic><topic>Poly A - genetics</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>RNA, Fungal - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LOSBERGER, C</creatorcontrib><creatorcontrib>ERNST, J. 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The C. albicans ODCase is most closely related to the ODCases of the budding yeasts Kluyveromyces lactis and S. cerevisiae (74% and 71% homology, respectively). Most 5' ends of URA3 transcripts in the authentic host and in the heterologous host S. cerevisiae were found to be identical. These results demonstrate a close taxonomic relationship between non-pathogenic budding yeasts and C. albicans.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>2574635</pmid><doi>10.1007/bf00391471</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0172-8083 |
| ispartof | Current genetics, 1989-09, Vol.16 (3), p.153-157 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Candida albicans - enzymology Candida albicans - genetics Carboxy-Lyases - genetics Fundamental and applied biological sciences. Psychology Genes. Genome Kluyveromyces - enzymology Kluyveromyces - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Orotidine-5'-Phosphate Decarboxylase - genetics Poly A - genetics Regulatory Sequences, Nucleic Acid RNA, Fungal - genetics RNA, Messenger - genetics Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Sequence Homology, Nucleic Acid |
| title | Sequence and transcript analysis of the C. albicans URA3 gene encoding orotidine-5'-phosphate decarboxylase |
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