Viruses with bisegmented double-stranded rna in pig faeces
Viruses similar to the bisegmented double-stranded (ds) rna picobirnaviruses described in human faeces and the intestinal contents of Oryzomys nigripes rats and guinea pigs were isolated from the faeces of pigs taken from several areas in the state of Sao Paulo, Brazil. Samples were collected from 9...
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| Veröffentlicht in: | Research in veterinary science 1989-11, Vol.47 (3), p.397-398 |
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| container_title | Research in veterinary science |
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| creator | GATTI, M.S.V. CASTRO, A.F. PESTANA DE FERRAZ, M.M.G. FIALFIO, A.M. PEREIRA, H.G. |
| description | Viruses similar to the bisegmented double-stranded (ds) rna picobirnaviruses described in human faeces and the intestinal contents of Oryzomys nigripes rats and guinea pigs were isolated from the faeces of pigs taken from several areas in the state of Sao Paulo, Brazil. Samples were collected from 912 pigs of several breeds, aged nine to 61 days, and assayed by polyacrylamide gel electrophoresis with silver staining and a combined enzyme immunoassay for rotavirus and adenovirus, using the simian rotavirus SA11 as control. Electrophoretic profiles resembling the bisegmented dsrna viruses were detected in 106 pigs with 15·3 per cent occurring in animals with diarrhoea compared to 9·6 per cent in animals without diarrhoea. |
| doi_str_mv | 10.1016/S0034-5288(18)31269-4 |
| format | Article |
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PESTANA DE ; FERRAZ, M.M.G. ; FIALFIO, A.M. ; PEREIRA, H.G.</creator><creatorcontrib>GATTI, M.S.V. ; CASTRO, A.F. PESTANA DE ; FERRAZ, M.M.G. ; FIALFIO, A.M. ; PEREIRA, H.G.</creatorcontrib><description>Viruses similar to the bisegmented double-stranded (ds) rna picobirnaviruses described in human faeces and the intestinal contents of Oryzomys nigripes rats and guinea pigs were isolated from the faeces of pigs taken from several areas in the state of Sao Paulo, Brazil. Samples were collected from 912 pigs of several breeds, aged nine to 61 days, and assayed by polyacrylamide gel electrophoresis with silver staining and a combined enzyme immunoassay for rotavirus and adenovirus, using the simian rotavirus SA11 as control. 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PESTANA DE</creatorcontrib><creatorcontrib>FERRAZ, M.M.G.</creatorcontrib><creatorcontrib>FIALFIO, A.M.</creatorcontrib><creatorcontrib>PEREIRA, H.G.</creatorcontrib><title>Viruses with bisegmented double-stranded rna in pig faeces</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Viruses similar to the bisegmented double-stranded (ds) rna picobirnaviruses described in human faeces and the intestinal contents of Oryzomys nigripes rats and guinea pigs were isolated from the faeces of pigs taken from several areas in the state of Sao Paulo, Brazil. Samples were collected from 912 pigs of several breeds, aged nine to 61 days, and assayed by polyacrylamide gel electrophoresis with silver staining and a combined enzyme immunoassay for rotavirus and adenovirus, using the simian rotavirus SA11 as control. Electrophoretic profiles resembling the bisegmented dsrna viruses were detected in 106 pigs with 15·3 per cent occurring in animals with diarrhoea compared to 9·6 per cent in animals without diarrhoea.</description><subject>Animals</subject><subject>Brazil</subject><subject>Diarrhea</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhea - veterinary</subject><subject>Double-stranded RNA</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme immunoassay</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>Gel electrophoresis</subject><subject>Guinea pigs</subject><subject>Immunoassay</subject><subject>Immunoenzyme Techniques</subject><subject>Intestine</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA viruses</subject><subject>RNA Viruses - genetics</subject><subject>RNA Viruses - isolation & purification</subject><subject>RNA, Double-Stranded - analysis</subject><subject>Rotavirus</subject><subject>Swine</subject><subject>Swine Diseases - microbiology</subject><subject>Veterinary medicine</subject><subject>Viruses</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LHEEQhhtRzGb1JwgDAUkOY_r7w4vIkqggeIjJtemPmk0vszOb7plI_r2z7rIHLzkVRT1vVfEgdEHwFcFEfv2BMeO1oFp_JvoLI1Samh-hGRGM1lRKcoxmB-QD-ljKCmPMCVGn6JRKrYwhM3T9K-WxQKle0vC78qnAcg3dALGK_ehbqMuQXRenPneuSl21ScuqcRCgnKGTxrUFzvd1jn5-__a8uK8fn-4eFrePdWBaD7VrGqewbII2hjNqnKJcGS9cxEEK570XEij2MjLiKOeKRy8lxcTLxhlG2Rxd7vZucv9nhDLYdSoB2tZ10I_FKsOU0mQLfnoHrvpxerstlmIhzCRDiYkSOyrkvpQMjd3ktHb5nyXYbs3aN7N2q80Sbd_MWj7lLvbbR7-GeEjtVU7zm90cJhd_E2RbQoIuQEwZwmBjn_5z4RUgR4aH</recordid><startdate>19891101</startdate><enddate>19891101</enddate><creator>GATTI, M.S.V.</creator><creator>CASTRO, A.F. 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PESTANA DE ; FERRAZ, M.M.G. ; FIALFIO, A.M. ; PEREIRA, H.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-affa706fc8994329a72479b5ad0c65abbb56e20b6d31a24474db66201b6fa9323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Brazil</topic><topic>Diarrhea</topic><topic>Diarrhea - microbiology</topic><topic>Diarrhea - veterinary</topic><topic>Double-stranded RNA</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme immunoassay</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>Gel electrophoresis</topic><topic>Guinea pigs</topic><topic>Immunoassay</topic><topic>Immunoenzyme Techniques</topic><topic>Intestine</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA viruses</topic><topic>RNA Viruses - genetics</topic><topic>RNA Viruses - isolation & purification</topic><topic>RNA, Double-Stranded - analysis</topic><topic>Rotavirus</topic><topic>Swine</topic><topic>Swine Diseases - microbiology</topic><topic>Veterinary medicine</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GATTI, M.S.V.</creatorcontrib><creatorcontrib>CASTRO, A.F. 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PESTANA DE</au><au>FERRAZ, M.M.G.</au><au>FIALFIO, A.M.</au><au>PEREIRA, H.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viruses with bisegmented double-stranded rna in pig faeces</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>1989-11-01</date><risdate>1989</risdate><volume>47</volume><issue>3</issue><spage>397</spage><epage>398</epage><pages>397-398</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>Viruses similar to the bisegmented double-stranded (ds) rna picobirnaviruses described in human faeces and the intestinal contents of Oryzomys nigripes rats and guinea pigs were isolated from the faeces of pigs taken from several areas in the state of Sao Paulo, Brazil. Samples were collected from 912 pigs of several breeds, aged nine to 61 days, and assayed by polyacrylamide gel electrophoresis with silver staining and a combined enzyme immunoassay for rotavirus and adenovirus, using the simian rotavirus SA11 as control. Electrophoretic profiles resembling the bisegmented dsrna viruses were detected in 106 pigs with 15·3 per cent occurring in animals with diarrhoea compared to 9·6 per cent in animals without diarrhoea.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>2687991</pmid><doi>10.1016/S0034-5288(18)31269-4</doi><tpages>2</tpages></addata></record> |
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| ispartof | Research in veterinary science, 1989-11, Vol.47 (3), p.397-398 |
| issn | 0034-5288 1532-2661 |
| language | eng |
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| subjects | Animals Brazil Diarrhea Diarrhea - microbiology Diarrhea - veterinary Double-stranded RNA Electrophoresis Electrophoresis, Polyacrylamide Gel Enzyme immunoassay Feces Feces - microbiology Gel electrophoresis Guinea pigs Immunoassay Immunoenzyme Techniques Intestine Ribonucleic acid RNA RNA viruses RNA Viruses - genetics RNA Viruses - isolation & purification RNA, Double-Stranded - analysis Rotavirus Swine Swine Diseases - microbiology Veterinary medicine Viruses |
| title | Viruses with bisegmented double-stranded rna in pig faeces |
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